product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Ganglioside GT1b
catalog :
GT1b-2a
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GT1b-2a
reactivity :
mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 3
Reference
Vajn K, Viljetić B, Degmecić I, Schnaar R, Heffer M. Differential distribution of major brain gangliosides in the adult mouse central nervous system. PLoS ONE. 2013;8:e75720 pubmed publisher
Gong Y, Tagawa Y, Lunn M, Laroy W, Heffer Lauc M, Li C, et al. Localization of major gangliosides in the PNS: implications for immune neuropathies. Brain. 2002;125:2491-506 pubmed
Schnaar R, Fromholt S, Gong Y, Vyas A, Laroy W, Wayman D, et al. Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides. Anal Biochem. 2002;302:276-84 pubmed
product information
Internal ID :
9459
Name :
GT1b-2a
Depositor Name :
Schnaar, R.L.
Depositor Institution :
Johns Hopkins School of Medicine
Date Deposited :
2/25/14
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Ganglioside GT1b
Antigen Species :
Bovine
Host Species :
mouse
Isotype :
MIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Human,Mouse,Rat,Vertebrates
Species Tested (legacy) :
recongizes all vertebrate species
Initial Publication Pubmed ID :
11878808
Depositor Notes (Special Instructions) :
GT1b-2a recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Collections :
Cell markers,Human,Neurodevelopment
Search Keywords :
Schnaar, R.L., Ganglioside GT1b, Bovine, MIgG2a, vertebrates/Human/Mouse/Rat, , , AB_2619574, monoclonal, Cell markers/Human/neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA
Antigen Molecular Weight :
2.15 kDa
Antibody Registry ID :
AB_2619574
Immunogen :
KLH-conjugated bovine brain GT1b
Clonality :
Monoclonal
Myeloma Strain :
P3x653
Epitope Mapped :
Yes
Epitope Location or Sequence :
GT1b
Epitope Map PubMed ID :
11878808
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
11878808 24098718
Immunofluorescence Pubmed IDs :
12390975 24098718
Western Blot Pubmed IDs :
11878808 12390975
ELISA Pubmed IDs :
11878808
Additional Information :
Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Neurochem. 75, 404-412.; Brain 125, 2491-2506.; Biochim. Biophys. Acta. 1686, 200-208.; J. Histochem. Cytochem. 55(8), 805-812.; PLoS ONE 8(9): e75720. doi:10.1371/journal.pone.0075720.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US