product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Ganglioside GT1b
catalog :
GT1b-1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GT1b-1
reactivity :
cow
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
more info or order :
citations: 7
| Reference |
|---|
Heffer Lauc M, Viljetić B, Vajn K, Schnaar R, Lauc G. Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections. J Histochem Cytochem. 2007;55:805-12 pubmed
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Heffer Lauc M, Lauc G, Nimrichter L, Fromholt S, Schnaar R. Membrane redistribution of gangliosides and glycosylphosphatidylinositol-anchored proteins in brain tissue sections under conditions of lipid raft isolation. Biochim Biophys Acta. 2005;1686:200-8 pubmed
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Gong Y, Tagawa Y, Lunn M, Laroy W, Heffer Lauc M, Li C, et al. Localization of major gangliosides in the PNS: implications for immune neuropathies. Brain. 2002;125:2491-506 pubmed
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Schnaar R, Fromholt S, Gong Y, Vyas A, Laroy W, Wayman D, et al. Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides. Anal Biochem. 2002;302:276-84 pubmed
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product information
Internal ID :
10030
Name :
GT1b-1
Depositor Name :
Schnaar, R.L.
Depositor Institution :
Johns Hopkins School of Medicine
Date Deposited :
6/13/14
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Ganglioside GT1b
Antigen Species :
Bovine
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Human,Mouse,Rat,Vertebrates
Species Tested (legacy) :
vertebrates
Initial Publication Pubmed ID :
11878808
Depositor Notes (Special Instructions) :
GT1b-1 recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Collections :
Cell markers,Human,Neurodevelopment
Search Keywords :
schnaar, Ganglioside GT1b, monoclonal, bovine, AB_2619573, IgG1, Human,Mouse,Rat,Vertebrates, ELISA,Immunofluorescence,Immunohistochemistry,Western Blot
Antigen Molecular Weight :
2.15 kDa
Antibody Registry ID :
AB_2619573
Immunogen :
KLH-conjugated bovine brain GT1b
Clonality :
Monoclonal
Myeloma Strain :
P3x653
Epitope Mapped :
Yes
Epitope Location or Sequence :
GT1b
Epitope Map PubMed ID :
11878808
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
11878808 24098718 22735313 22206893
Immunofluorescence Pubmed IDs :
12390975 24098718
Western Blot Pubmed IDs :
11878808 12390975
ELISA Pubmed IDs :
11878808
Depositor Growth Medium :
per 100 ml: 88 ml DMEM; 1 ml ÒHIPOÓ concentrate (1 M Hepes, 20 U/ml insulin, 0.35 ?l/ml ??mercaptoethanol, 15 mg/ml oxalacetic acid, 5 mg/mlsodium pyruvate); 1 ml 200 mM glutamine; 10 ml fetal bovine serum
Additional Information :
Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
DSHB Growth Medium :
Iscove's
References (legacy) :
. J. Neurochem. 75, 404-412.; Brain 125(11), 2491-2506.; Biochim. Biophys. Acta. 1686, 200-208.; J. Histochem. Cytochem. 55(8), 805-812.; PLoS ONE 8(9): e75720. doi:10.1371/journal.pone.0075720.
more info or order :
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