product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
insulin (proinsulin)
catalog :
GS-9A8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GS-9A8
reactivity :
human, mouse, rat, dog
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
more info or order :
Developmental Studies Hybridoma Bank product webpage
citations: 17
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; human; fig 3
Dorrell C, Schug J, Canaday P, Russ H, Tarlow B, Grompe M, et al. Human islets contain four distinct subtypes of ? cells. Nat Commun. 2016;7:11756 pubmed publisher
Balboa D, Saarimäki Vire J, Borshagovski D, Survila M, Lindholm P, Galli E, et al. Insulin mutations impair beta-cell development in a patient-derived iPSC model of neonatal diabetes. elife. 2018;7: pubmed publisher
Puri S, Roy N, Russ H, Leonhardt L, French E, Roy R, et al. Replication confers β cell immaturity. Nat Commun. 2018;9:485 pubmed publisher
Wasserfall C, Nick H, Campbell Thompson M, Beachy D, Haataja L, Kusmartseva I, et al. Persistence of Pancreatic Insulin mRNA Expression and Proinsulin Protein in Type 1 Diabetes Pancreata. Cell Metab. 2017;26:568-575.e3 pubmed publisher
Rodríguez Calvo T, Zapardiel Gonzalo J, Amirian N, Castillo E, Lajevardi Y, Krogvold L, et al. Increase in Pancreatic Proinsulin and Preservation of β-Cell Mass in Autoantibody-Positive Donors Prior to Type 1 Diabetes Onset. Diabetes. 2017;66:1334-1345 pubmed publisher
Pérez Pevida B, Idoate M, Fernández Landázuri S, Varo N, Escalada J. Hypoglycemic Syndrome without Hyperinsulinemia. A Diagnostic Challenge. Endocr Pathol. 2016;27:50-4 pubmed publisher
Suriben R, Kaihara K, Paolino M, Reichelt M, Kummerfeld S, Modrusan Z, et al. β-Cell Insulin Secretion Requires the Ubiquitin Ligase COP1. Cell. 2015;163:1457-67 pubmed publisher
Valli V, Blandamura S, Pastorelli D, Merigliano S, Sperti C. Nesidioblastosis coexisting with non-functioning islet cell tumour in an adult. Endokrynol Pol. 2015;66:356-60 pubmed publisher
Wernersson R, Frogne T, Rescan C, Hansson L, Bruun C, Grønborg M, et al. Analysis artefacts of the INS-IGF2 fusion transcript. BMC Mol Biol. 2015;16:13 pubmed publisher
Asadi A, Bruin J, Kieffer T. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem. 2015;63:646-62 pubmed publisher
Alshahrani S, Di Fulvio M. Enhanced insulin secretion and improved glucose tolerance in mice with homozygous inactivation of the Na(+)K(+)2Cl(-) co-transporter 1. J Endocrinol. 2012;215:59-70 pubmed publisher
Kluth O, Mirhashemi F, Scherneck S, Kaiser D, Kluge R, Neschen S, et al. Dissociation of lipotoxicity and glucotoxicity in a mouse model of obesity associated diabetes: role of forkhead box O1 (FOXO1) in glucose-induced beta cell failure. Diabetologia. 2011;54:605-16 pubmed publisher
Madsen O, Frank B, Steiner D. Human proinsulin-specific antigenic determinants identified by monoclonal antibodies. Diabetes. 1984;33:1012-6 pubmed
Madsen O, Cohen R, Fitch F, Rubenstein A, Steiner D. The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure. Endocrinology. 1983;113:2135-44 pubmed
Orci L, Ravazzola M, Amherdt M, Madsen O, Vassalli J, Perrelet A. Direct identification of prohormone conversion site in insulin-secreting cells. Cell. 1985;42:671-81 pubmed
Orci L, Ravazzola M, Storch M, Anderson R, Vassalli J, Perrelet A. Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles. Cell. 1987;49:865-8 pubmed
Madsen O. Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion. Diabetes. 1987;36:1203-11 pubmed
product information
Internal ID :
3378
Name :
GS-9A8
Depositor Name :
Madsen, O.D.
Depositor Institution :
Hagedorn Research Institute
Date Deposited :
1/23/06
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
insulin (proinsulin)
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Canine,Human,Monkey,Mouse,Rat
Species Tested (legacy) :
human and monkey
Initial Publication Pubmed ID :
6196183
Depositor Notes (Special Instructions) :
This antibody binds to the B-C junction of proinsulin of human and monkey and stains only intact, non-processed proinsulin found in the immature secretory granules of islet beta-cells. It does not cross-react to insulin nor C-peptide. Cross reaction to rodent proinsulins requires aldehyde fixation.
Collections :
Cell markers,Cell signaling,Enzymes,Human,Stem cell
Search Keywords :
Madsen, O.D., insulin (proinsuline), Human, MIgG1, Human/Monkey/rat/mouse/canine, INS, , AB_532383, monoclonal, Cell signaling/Human/Stem cell/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: 9.4 kDa
Gene :
INS
Uniprot ID :
P01308
Antibody Registry ID :
AB_532383
Immunogen :
Recombinant human proinsulin coupled to bovine thyroglobulin
Clonality :
Monoclonal
Myeloma Strain :
SP2/0-Ag14
Epitope Mapped :
Yes
Epitope Location or Sequence :
B-C junction of proinsulin (aa 26-37)
Epitope Map PubMed ID :
2443408 6207064
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunohistochemistry Pubmed IDs :
3555846 2443408 26801953 26323473
Immunofluorescence Pubmed IDs :
3896518 2443408 27399229 26220792 26216140 26627735 22872759 28877460
Western Blot Pubmed IDs :
26220792
Immunoprecipitation Pubmed IDs :
6196183
Additional Information :
Doesn't recognize bovine or porcine proinsulin.
DSHB Growth Medium :
RPMI
References (legacy) :
Endocrinology 113(6), 2135-2144.; Cell 42(2), 671-681.; Cell 49(6), 865-868.; Diabetes 36(10), 1203-1211.
more info or order :
Developmental Studies Hybridoma Bank product webpage
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US