insulin (proinsulin; C-peptide) | Developmental Studies Hybridoma Bank GN-ID4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

insulin (proinsulin; C-peptide)

catalog :

GN-ID4

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

GN-ID4

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:100; fig s3c	Low B, Lim C, Ding S, Tan Y, Ng N, Krishnan V, et al. Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Nat Commun. 2021;12:3133 pubmed publisher
flow cytometry; human; loading ...; fig 7d	Yung T, Poon F, Liang M, Coquenlorge S, McGaugh E, Hui C, et al. Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals. Nat Commun. 2019;10:4647 pubmed publisher
immunohistochemistry - frozen section; human; 1:100; loading ...; fig e1a flow cytometry; human; 1:100; loading ...; fig 1a, s3b	Veres A, Faust A, Bushnell H, Engquist E, Kenty J, Harb G, et al. Charting cellular identity during human in vitro β-cell differentiation. Nature. 2019;569:368-373 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 7b	Sharon N, Chawla R, Mueller J, Vanderhooft J, Whitehorn L, Rosenthal B, et al. A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets. Cell. 2019;176:790-804.e13 pubmed publisher
flow cytometry; human; 1:500; fig 4b immunocytochemistry; human; 1:2000; fig 5b	Shi Z, Lee K, Yang D, Amin S, Verma N, Li Q, et al. Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development. Cell Stem Cell. 2017;20:675-688.e6 pubmed publisher
flow cytometry; human; 1:50; fig s20 immunocytochemistry; human; 1:50; fig 3	Saxena P, Heng B, Bai P, Folcher M, Zulewski H, Fussenegger M. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells. Nat Commun. 2016;7:11247 pubmed publisher
immunohistochemistry - paraffin section; human; fig 3	Vegas A, Veiseh O, Gürtler M, Millman J, Pagliuca F, Bader A, et al. Long-term glycemic control using polymer-encapsulated human stem cell-derived beta cells in immune-competent mice. Nat Med. 2016;22:306-11 pubmed publisher
flow cytometry; human; 1:1000; fig 3	Korytnikov R, Nostro M. Generation of polyhormonal and multipotent pancreatic progenitor lineages from human pluripotent stem cells. Methods. 2016;101:56-64 pubmed publisher
	Leite N, Sintov E, Meissner T, Brehm M, Greiner D, Harlan D, et al. Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells. Cell Rep. 2020;32:107894 pubmed publisher
	Ramond C, Beydag Tasöz B, Azad A, van de Bunt M, Petersen M, Beer N, et al. Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling. Development. 2018;145: pubmed publisher
	Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
	Dirice E, Walpita D, Vetere A, Meier B, Kahraman S, Hu J, et al. Inhibition of DYRK1A Stimulates Human Î²-Cell Proliferation. Diabetes. 2016;65:1660-71 pubmed publisher
	Braunstein I, Zach L, Allan S, Kalies K, Stanhill A. Proteasomal degradation of preemptive quality control (pQC) substrates is mediated by an AIRAPL-p97 complex. Mol Biol Cell. 2015;26:3719-27 pubmed publisher
	Asadi A, Bruin J, Kieffer T. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem. 2015;63:646-62 pubmed publisher
	Nostro M, Sarangi F, Yang C, Holland A, Elefanty A, Stanley E, et al. Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. Stem Cell Reports. 2015;4:591-604 pubmed publisher
	Sui L, Geens M, Sermon K, Bouwens L, Mfopou J. Role of BMP signaling in pancreatic progenitor differentiation from human embryonic stem cells. Stem Cell Rev. 2013;9:569-77 pubmed publisher
	Brolén G, Heins N, Edsbagge J, Semb H. Signals from the embryonic mouse pancreas induce differentiation of human embryonic stem cells into insulin-producing beta-cell-like cells. Diabetes. 2005;54:2867-74 pubmed
	Itier J, Douhet P, Desbois P, Joshi R, Dandoy Dron F, Jami J, et al. Human insulin gene expression in transgenic mice: mutational analysis of the regulatory region. Differentiation. 1996;60:309-16 pubmed
	Douhet P, Destrade C, Bucchini D, Calas A. Expression of a human insulin transgene in cholinergic neurons of the mouse medial habenula. Biol Cell. 1995;85:137-46 pubmed
	Madsen O, Cohen R, Fitch F, Rubenstein A, Steiner D. The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure. Endocrinology. 1983;113:2135-44 pubmed
	Madsen O, Andersen L, Michelsen B, Owerbach D, Larsson L, Lernmark A, et al. Tissue-specific expression of transfected human insulin genes in pluripotent clonal rat insulinoma lines induced during passage in vivo. Proc Natl Acad Sci U S A. 1988;85:6652-6 pubmed
	Madsen O. Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion. Diabetes. 1987;36:1203-11 pubmed
	Deltour L, Leduque P, Paldi A, Ripoche M, DuBois P, Jami J. Polyclonal origin of pancreatic islets in aggregation mouse chimaeras. Development. 1991;112:1115-21 pubmed
	Widagdo -, Sunardi -, Lokollo D, Margono S. Ocular angiostrongyliasis in Semarang, Central Java. Am J Trop Med Hyg. 1977;26:72-4 pubmed

product information

Internal ID :

3373

Name :

GN-ID4

Depositor Name :

Madsen, O.D.

Depositor Institution :

Hagedorn Research Institute

Date Deposited :

1/23/06

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

insulin (proinsulin; C-peptide)

Antigen Species :

Human

Host Species :

rat

Isotype :

RIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Human,Monkey

Species Tested (legacy) :

human and monkey

Initial Publication Pubmed ID :

6196183

Depositor Notes (Special Instructions) :

This antibody recognizes the C-peptide (aa 33-63 of proinsulin) which separates insulin B chain (aa 1-30) from insulin A chain (aa 66-86) in the proinsulin protein (minus signal peptide sequence). Stains C-peptide in mature granules and proinsulin in immature granules of islet beta-cells. The antibody does not cross-react with rodent C-peptide/proinsulin.

Collections :

Cell markers,Cell signaling,Enzymes,Human,Stem cell

Search Keywords :

Madsen, Ole D., insulin (proinsulin; C-peptide), Human, RIgG2a, Human/Monkey, INS, , AB_2255626, monoclonal

Antigen Molecular Weight :

Predicted: 9.4 kDa (proinsulin) and 3 kDa (C-peptide)

Gene :

INS

Uniprot ID :

P01308

Antibody Registry ID :

AB_2255626

Immunogen :

Recombinant human proinsulin coupled to bovine thyroglobulin

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0-Ag14

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-peptide (aa 40-45 and 57-63 of proinsulin)

Epitope Map PubMed ID :

6196183

Recommended Applications :

FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunohistochemistry Pubmed IDs :

2842785 2443408 1682130 842785

Immunofluorescence Pubmed IDs :

23468018 26216140 29249360

Immunoprecipitation Pubmed IDs :

6196183

FACS Pubmed IDs :

26515645

Pubmed IDs :

8855374 8785515

Depositor Growth Medium :

RPMI 1640 10% FCS, Pen/Strep & Fungizone

Additional Information :

This antibody stains unfixed frozen sections as well as frozen and paraffin sections of pancreas fixed with 4% PFA. Doesn't recognize bovine, canine or porcine proinsulin.

DSHB Growth Medium :

RPMI

References (legacy) :

Endocrinology 113(6), 2135-2144.; Diabetes 36(10), 1203-1211.; Proc. Natl. Acad. Sci. USA 85(18), 6652-6656.; Diabetes 54(10), 2867-2874.

more info or order :

Developmental Studies Hybridoma Bank product webpage