Ganglioside GM1 | Developmental Studies Hybridoma Bank GM1-1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Ganglioside GM1

catalog :

GM1-1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GM1-1

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 7

Reference
Vajn K, Viljetić B, Degmecić I, Schnaar R, Heffer M. Differential distribution of major brain gangliosides in the adult mouse central nervous system. PLoS ONE. 2013;8:e75720 pubmed publisher
Sturgill E, Aoki K, Lopez P, Colacurcio D, Vajn K, Lorenzini I, et al. Biosynthesis of the major brain gangliosides GD1a and GT1b. Glycobiology. 2012;22:1289-301 pubmed publisher
Viljetić B, Labak I, Majić S, Stambuk A, Heffer M. Distribution of mono-, di- and trisialo gangliosides in the brain of Actinopterygian fishes. Biochim Biophys Acta. 2012;1820:1437-43 pubmed publisher
Heffer Lauc M, Viljetić B, Vajn K, Schnaar R, Lauc G. Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections. J Histochem Cytochem. 2007;55:805-12 pubmed
Heffer Lauc M, Lauc G, Nimrichter L, Fromholt S, Schnaar R. Membrane redistribution of gangliosides and glycosylphosphatidylinositol-anchored proteins in brain tissue sections under conditions of lipid raft isolation. Biochim Biophys Acta. 2005;1686:200-8 pubmed
Gong Y, Tagawa Y, Lunn M, Laroy W, Heffer Lauc M, Li C, et al. Localization of major gangliosides in the PNS: implications for immune neuropathies. Brain. 2002;125:2491-506 pubmed
Schnaar R, Fromholt S, Gong Y, Vyas A, Laroy W, Wayman D, et al. Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides. Anal Biochem. 2002;302:276-84 pubmed

product information

Internal ID :

9402

Name :

GM1-1

Depositor Name :

Schnaar, R.L.

Depositor Institution :

Johns Hopkins School of Medicine

Date Deposited :

3/25/14

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Ganglioside GM1

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Mouse,Rat,Vertebrates

Species Tested (legacy) :

all vertebrates

Initial Publication Pubmed ID :

11878808

Depositor Notes (Special Instructions) :

GM1-1 recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.

Collections :

Cell markers,Human,Neurodevelopment

Search Keywords :

Schnaar, R.L., Ganglioside GM1, Bovine, MIgG1, vertebrates/Human/Mouse/Rat, , , AB_2619571, monoclonal, Cell markers/Human/neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA

Antigen Molecular Weight :

1.55 kDa

Antibody Registry ID :

AB_2619571

Immunogen :

KLH-conjugated bovine brain GM1

Clonality :

Monoclonal

Myeloma Strain :

P3x653

Epitope Mapped :

Yes

Epitope Location or Sequence :

GM1

Epitope Map PubMed ID :

11878808

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

11878808 15629689 17409378 24098718 22735313 22206893 30391320

Immunofluorescence Pubmed IDs :

12390975 24098718

Western Blot Pubmed IDs :

11878808 12390975

ELISA Pubmed IDs :

11878808

Additional Information :

Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Neurochem. 75, 404-412.; Brain 125, 2491-2506.; Biochim. Biophys. Acta. 1686, 200-208.; J. Histochem. Cytochem. 55(8), 805-812.; PLoS ONE 8(9): e75720. doi:10.1371/journal.pone.0075720.

more info or order :

Developmental Studies Hybridoma Bank product webpage