LAMP-2 (mouse) | Developmental Studies Hybridoma Bank GL2A7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

LAMP-2 (mouse)

catalog :

GL2A7

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

GL2A7

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig s7b	Conlon D, Thomas T, Fedotova T, Hernandez Ono A, Di Paolo G, Chan R, et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J Clin Invest. 2016;126:3852-3867 pubmed publisher
immunocytochemistry; mouse; fig 9a	Dennis M, Delevoye C, Acosta Ruiz A, Hurbain I, Romao M, Hesketh G, et al. BLOC-1 and BLOC-3 regulate VAMP7 cycling to and from melanosomes via distinct tubular transport carriers. J Cell Biol. 2016;214:293-308 pubmed publisher
immunocytochemistry; mouse; fig 2	Dennis M, Mantegazza A, Snir O, Tenza D, Acosta Ruiz A, Delevoye C, et al. BLOC-2 targets recycling endosomal tubules to melanosomes for cargo delivery. J Cell Biol. 2015;209:563-77 pubmed publisher
immunocytochemistry; mouse; fig 7	Peng M, Yin N, Li M. Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling. Cell. 2014;159:122-133 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; fig 2	Tyzack G, Sitnikov S, Barson D, Adams Carr K, Lau N, Kwok J, et al. Astrocyte response to motor neuron injury promotes structural synaptic plasticity via STAT3-regulated TSP-1 expression. Nat Commun. 2014;5:4294 pubmed publisher
	Sarkar C, Jones J, Hegdekar N, Thayer J, Kumar A, Faden A, et al. PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma. Autophagy. 2019;:1-20 pubmed publisher
	Venkatesh A, Ma S, Punzo C. TSC but not PTEN loss in starving cones of retinitis pigmentosa mice leads to an autophagy defect and mTORC1 dissociation from the lysosome. Cell Death Dis. 2016;7:e2279 pubmed publisher
	Bellono N, Escobar I, Oancea E. A melanosomal two-pore sodium channel regulates pigmentation. Sci Rep. 2016;6:26570 pubmed publisher
	Jani R, Purushothaman L, Rani S, Bergam P, Setty S. STX13 regulates cargo delivery from recycling endosomes during melanosome biogenesis. J Cell Sci. 2015;128:3263-76 pubmed publisher
	Bellono N, Escobar I, Lefkovith A, Marks M, Oancea E. An intracellular anion channel critical for pigmentation. elife. 2014;3:e04543 pubmed publisher
	Stewart R, Teng H, Wilkinson R. "Late" macroendosomes and acidic endosomes in vertebrate motor nerve terminals. J Comp Neurol. 2012;520:4275-93 pubmed publisher
	Punzo C, Kornacker K, Cepko C. Stimulation of the insulin/mTOR pathway delays cone death in a mouse model of retinitis pigmentosa. Nat Neurosci. 2009;12:44-52 pubmed publisher
	Sun Wada G, Toyomura T, Murata Y, Yamamoto A, Futai M, Wada Y. The a3 isoform of V-ATPase regulates insulin secretion from pancreatic beta-cells. J Cell Sci. 2006;119:4531-40 pubmed
	Shirahama Noda K, Yamamoto A, Sugihara K, Hashimoto N, Asano M, Nishimura M, et al. Biosynthetic processing of cathepsins and lysosomal degradation are abolished in asparaginyl endopeptidase-deficient mice. J Biol Chem. 2003;278:33194-9 pubmed
	Nakamura N, Yamamoto A, Wada Y, Futai M. Syntaxin 7 mediates endocytic trafficking to late endosomes. J Biol Chem. 2000;275:6523-9 pubmed
	Drake J, Lewis T, Condon K, Mitchell R, Webster P. Involvement of MIIC-like late endosomes in B cell receptor-mediated antigen processing in murine B cells. J Immunol. 1999;162:1150-5 pubmed
	Blyth Bristow B. Implementing a smoking policy at work--a case study. Occup Health (Lond). 1989;41:163-5 pubmed
	Granger B, Green S, Gabel C, Howe C, Mellman I, Helenius A. Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells. J Biol Chem. 1990;265:12036-43 pubmed

product information

Internal ID :

3664

Name :

GL2A7

Depositor Name :

Granger, B.L.

Depositor Institution :

Montana State University

Date Deposited :

1/9/97

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

LAMP-2 (mouse)

Antigen Species :

Mouse

Host Species :

rat

Isotype :

RIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a, kappa light chain

Positive Tested Species Reactivity :

Mouse

Species Tested (legacy) :

mouse, not rat, hamster, or primate

Initial Publication Pubmed ID :

2142158

Depositor Notes (Special Instructions) :

Doesn't recognize rat, hamster or primate antigen. GL2A7 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.

Collections :

CD antigens,Cell signaling

Search Keywords :

Granger, B.L., LAMP-2 (mouse), Mouse, RIgG2a, kappa light chain, Mouse, Lamp2, CD107b, Mac3, LGP-B, Igp110, AB_2314734, monoclonal, Cell signaling/Cluster Determinant antigens, Immunoprecipitation/Western Blot/Immunofluorescence

Antigen Molecular Weight :

Predicted: 43 kDa; Apparent: 110 kDa

Gene :

Lamp2

Alternate Gene Name(s) :

CD107b, Mac3, LGP-B, Igp110

Uniprot ID :

P17047

Antibody Registry ID :

AB_2314734

Synonyms (Alternate Clone Names) :

CD107b

Immunogen :

Mouse lysosomes (2% glutaraldehyde fixed) isolated by Percoll gradient fractionation of liver homogenate

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3U1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Lumenal region

Epitope Map PubMed ID :

2142158

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

2142158 17046993 9916746 12775715 19060896 10692457 26208634 27362797 27231233 27599291 27482051 2740045

Western Blot Pubmed IDs :

2142158 9916746

Immunoprecipitation Pubmed IDs :

2142158

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Biol. Chem. 265, 12036-12043.; Cell & Mol. Biol. Res. 41(5), 405-420.

more info or order :

Developmental Studies Hybridoma Bank product webpage