product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Green fluorescent protein (GFP) variant
catalog :
GFP-G1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GFP-G1
application :
immunohistochemistry, immunocytochemistry
citations: 5
Reference
Ding Z, Wang Y, Huang Y, Lee M, Tseng M, Chi Y, et al. Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture. J Cell Biol. 2017;216:2827-2841 pubmed publisher
Collins M, Mandigo T, Camuglia J, Vazquez G, Anderson A, Hudson C, et al. Emery-Dreifuss muscular dystrophy-linked genes and centronuclear myopathy-linked genes regulate myonuclear movement by distinct mechanisms. Mol Biol Cell. 2017;28:2303-2317 pubmed publisher
Brown M, Andrzejewski D, Burnside A, Schneyer A. Activin Enhances α- to β-Cell Transdifferentiation as a Source For β-Cells In Male FSTL3 Knockout Mice. Endocrinology. 2016;157:1043-54 pubmed publisher
Manhire Heath R, Golenkina S, Saint R, Murray M. Netrin-dependent downregulation of Frazzled/DCC is required for the dissociation of the peripodial epithelium in Drosophila. Nat Commun. 2013;4:2790 pubmed publisher
Yamagata M, Sanes J. Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP). Front Mol Neurosci. 2012;5:18 pubmed publisher
product information
Internal ID :
8326
Name :
GFP-G1
Depositor Name :
Sanes, J.R. / Yamagata, M.
Depositor Institution :
Harvard University
Depositor Website :
http://saneslab.mcb.harvard.edu/
Date Deposited :
12/7/12
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Green fluorescent protein (GFP) variant
Antigen Species :
Aequorea victoria
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Aequorea victoria,All species for fusion proteins
Species Tested (legacy) :
all derivatives of Aequorea victoria tested plus all species of GFP-fusion proteins
Initial Publication Pubmed ID :
22355283
Depositor Notes (Special Instructions) :
GFP-G1 does not western blot.
Collections :
Protein tags
Search Keywords :
Joshua R. Sanes / Masahito Yamagata, Green fluorescent protein (GFP) variant, Aequorea victoria, MIgG1, kappa light chain, Aequorea victoria/All species for fusion proteins, GFP, , , Monoclonal
Antigen Molecular Weight :
26.8 kDa
Gene :
GFP
Uniprot ID :
P42212
Antibody Registry ID :
AB_2619561
Immunogen :
Recombinant protein
Immunogen Sequence :
Full length
Clonality :
Monoclonal
Myeloma Strain :
FOX-NY
Epitope Mapped :
Yes
Epitope Location or Sequence :
GFP1-10 fragment
Epitope Map PubMed ID :
22355283
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunoblotting (legacy) :
no
Immunohistochemistry Pubmed IDs :
24225841
Immunofluorescence Pubmed IDs :
22355283
Depositor Growth Medium :
Hybridoma-SFM (Gibco)
Additional Information :
GFP-G1 recognizes all GFP derivatives tested (EGFP, Venus, EYFP, ECFP/cerulean) including the GFP1-10 fragment. Paraformaldehyde (4%) or methanol fixation is recommended. RRID:AB_2619561
DSHB Growth Medium :
Iscove's
References (legacy) :
Front. Mol. Neurosci. 5:18. doi: 10.3389/fnmol.2012.00018.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US