product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Ganglioside GD1a
catalog :
GD1a-2b
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GD1a-2b
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 8
Reference
Vajn K, Viljetić B, Degmecić I, Schnaar R, Heffer M. Differential distribution of major brain gangliosides in the adult mouse central nervous system. PLoS ONE. 2013;8:e75720 pubmed publisher
Lopez P, Zhang G, Bianchet M, Schnaar R, Sheikh K. Structural requirements of anti-GD1a antibodies determine their target specificity. Brain. 2008;131:1926-39 pubmed publisher
Heffer Lauc M, Viljetić B, Vajn K, Schnaar R, Lauc G. Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections. J Histochem Cytochem. 2007;55:805-12 pubmed
Heffer Lauc M, Lauc G, Nimrichter L, Fromholt S, Schnaar R. Membrane redistribution of gangliosides and glycosylphosphatidylinositol-anchored proteins in brain tissue sections under conditions of lipid raft isolation. Biochim Biophys Acta. 2005;1686:200-8 pubmed
Zhang G, Lopez P, Li C, Mehta N, Griffin J, Schnaar R, et al. Anti-ganglioside antibody-mediated neuronal cytotoxicity and its protection by intravenous immunoglobulin: implications for immune neuropathies. Brain. 2004;127:1085-100 pubmed
Gong Y, Tagawa Y, Lunn M, Laroy W, Heffer Lauc M, Li C, et al. Localization of major gangliosides in the PNS: implications for immune neuropathies. Brain. 2002;125:2491-506 pubmed
Tagawa Y, Laroy W, Nimrichter L, Fromholt S, Moser A, Moser H, et al. Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids. Neurochem Res. 2002;27:847-55 pubmed
Schnaar R, Fromholt S, Gong Y, Vyas A, Laroy W, Wayman D, et al. Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides. Anal Biochem. 2002;302:276-84 pubmed
product information
Internal ID :
9384
Name :
GD1a-2b
Depositor Name :
Schnaar, R.L.
Depositor Institution :
Johns Hopkins School of Medicine
Date Deposited :
2/25/14
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Ganglioside GD1a
Antigen Species :
Bovine
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Human,Mouse,Rat,Vertebrates
Species Tested (legacy) :
recongizes all vertebrate species
Initial Publication Pubmed ID :
11878808
Depositor Notes (Special Instructions) :
GD1a-2b recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Collections :
Cell markers,Human,Neurodevelopment
Search Keywords :
Schnaar, R.L., Ganglioside GD1a, Bovine, MIgG2b, vertebrates/Human/Mouse/Rat, , , AB_2619569, monoclonal, Cell markers/Human/neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA
Antigen Molecular Weight :
1.84 kDa
Antibody Registry ID :
AB_2619569
Immunogen :
KLH-conjugated bovine brain GD1a
Clonality :
Monoclonal
Myeloma Strain :
P3x653
Epitope Mapped :
Yes
Epitope Location or Sequence :
GD1a
Epitope Map PubMed ID :
11878808
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
11878808 12390975 15629689 17409378 24098718
Immunofluorescence Pubmed IDs :
12390975 2409871818487279
Western Blot Pubmed IDs :
11878808 12390975 18487279
ELISA Pubmed IDs :
11878808 18487279 12374222
Pubmed IDs :
14985267
Additional Information :
Gangliosides are glycosphingolipids with one or more sialic acids. GD1a-2b cross-react with GT1aalpha [PMIDs: 11878808 12390975]. Western blot references include references for TLC immunooverlay.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Neurochem. 75, 404-412.; Brain 125, 2491-2506.; Biochim. Biophys. Acta. 1686, 200-208.; J. Histochem. Cytochem. 55(8), 805-812.; PLoS ONE 8(9): e75720. doi:10.1371/journal.pone.0075720.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US