Ganglioside GD1a | Developmental Studies Hybridoma Bank GD1a-2a product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Ganglioside GD1a

catalog :

GD1a-2a

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GD1a-2a

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Reference
Vajn K, Viljetić B, Degmecić I, Schnaar R, Heffer M. Differential distribution of major brain gangliosides in the adult mouse central nervous system. PLoS ONE. 2013;8:e75720 pubmed publisher
Lopez P, Zhang G, Bianchet M, Schnaar R, Sheikh K. Structural requirements of anti-GD1a antibodies determine their target specificity. Brain. 2008;131:1926-39 pubmed publisher
Buchwald B, Zhang G, Vogt Eisele A, Zhang W, Ahangari R, Griffin J, et al. Anti-ganglioside antibodies alter presynaptic release and calcium influx. Neurobiol Dis. 2007;28:113-21 pubmed
Heffer Lauc M, Viljetić B, Vajn K, Schnaar R, Lauc G. Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections. J Histochem Cytochem. 2007;55:805-12 pubmed
Perera V, Nachamkin I, Ung H, Patterson J, McConville M, Coloe P, et al. Molecular mimicry in Campylobacter jejuni: role of the lipo-oligosaccharide core oligosaccharide in inducing anti-ganglioside antibodies. FEMS Immunol Med Microbiol. 2007;50:27-36 pubmed
Heffer Lauc M, Lauc G, Nimrichter L, Fromholt S, Schnaar R. Membrane redistribution of gangliosides and glycosylphosphatidylinositol-anchored proteins in brain tissue sections under conditions of lipid raft isolation. Biochim Biophys Acta. 2005;1686:200-8 pubmed
Zhang G, Lopez P, Li C, Mehta N, Griffin J, Schnaar R, et al. Anti-ganglioside antibody-mediated neuronal cytotoxicity and its protection by intravenous immunoglobulin: implications for immune neuropathies. Brain. 2004;127:1085-100 pubmed
Gong Y, Tagawa Y, Lunn M, Laroy W, Heffer Lauc M, Li C, et al. Localization of major gangliosides in the PNS: implications for immune neuropathies. Brain. 2002;125:2491-506 pubmed
Tagawa Y, Laroy W, Nimrichter L, Fromholt S, Moser A, Moser H, et al. Anti-ganglioside antibodies bind with enhanced affinity to gangliosides containing very long chain fatty acids. Neurochem Res. 2002;27:847-55 pubmed
Schnaar R, Fromholt S, Gong Y, Vyas A, Laroy W, Wayman D, et al. Immunoglobulin G-class mouse monoclonal antibodies to major brain gangliosides. Anal Biochem. 2002;302:276-84 pubmed

product information

Internal ID :

9462

Name :

GD1a-2a

Depositor Name :

Schnaar, R.L.

Depositor Institution :

Johns Hopkins School of Medicine

Date Deposited :

2/25/14

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Ganglioside GD1a

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG2c

Isotype for catalog (legacy) :

IgG2c

Positive Tested Species Reactivity :

Campylobacter,Human,Mouse,Rat,Vertebrates

Species Tested (legacy) :

recongizes all vertebrate species

Initial Publication Pubmed ID :

11878808

Depositor Notes (Special Instructions) :

GD1a-2a recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.

Collections :

Cell markers,Human,Neurodevelopment

Search Keywords :

Schnaar, R.L., Ganglioside GD1a, Bovine, MIgG2c, vertebrates/Human/Mouse/Rat/Campylobacter, , , AB_2619568, monoclonal, Cell markers/Human/neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA/Function Blocking

Antigen Molecular Weight :

1.84 kDa

Antibody Registry ID :

AB_2619568

Immunogen :

KLH-conjugated bovine brain GD1a

Clonality :

Monoclonal

Myeloma Strain :

P3x653

Epitope Mapped :

Yes

Epitope Location or Sequence :

GD1a

Epitope Map PubMed ID :

11878808

Recommended Applications :

ELISA,Function Blocking,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

11878808 15629689 17409378 24098718

Immunofluorescence Pubmed IDs :

12390975 24098718 14985267 18487279

Western Blot Pubmed IDs :

11878808 12390975 17374131 14985267 18487279

ELISA Pubmed IDs :

11878808 18487279 12374222

Function Blocking Pubmed IDs :

17720506

Additional Information :

Gangliosides are glycosphingolipids with one or more sialic acids. GD1a-2a binds better to GT1aalpha [PMIDs: 11878808 12390975]. Western blot references are for TLC immunooverlay. The isotype of this antibody was originally described as MIgG2a.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Neurochem. 75, 404-412.; Brain 125, 2491-2506.; Biochim. Biophys. Acta. 1686, 200-208.; J. Histochem. Cytochem. 55(8), 805-812.; PLoS ONE 8(9): e75720. doi:10.1371/journal.pone.0075720.

more info or order :

Developmental Studies Hybridoma Bank product webpage