Glutamic acid decarboxylase; GAD | Developmental Studies Hybridoma Bank GAD-6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Glutamic acid decarboxylase; GAD

catalog :

GAD-6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GAD-6

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 60

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; rat; loading ...; fig 4k immunohistochemistry; rat; 1:25,000; loading ...; fig 4h	Rahman A, Weber J, Labin E, Lai C, Prieto A. Developmental expression of Neuregulin-3 in the rat central nervous system. J Comp Neurol. 2019;527:797-817 pubmed publisher
immunohistochemistry; rat; 1:200; loading ...; tbl 1	Schultz A, Rotterman T, Dwarakanath A, Alvarez F. VGLUT1 synapses and P-boutons on regenerating motoneurons after nerve crush. J Comp Neurol. 2017;525:2876-2889 pubmed publisher
western blot; mouse; 1:500; fig s7e	Sterky F, Trotter J, Lee S, Recktenwald C, Du X, Zhou B, et al. Carbonic anhydrase-related protein CA10 is an evolutionarily conserved pan-neurexin ligand. Proc Natl Acad Sci U S A. 2017;114:E1253-E1262 pubmed publisher
immunocytochemistry; rat; 1:100; loading ...; fig 1a immunohistochemistry; mouse; 1:100; loading ...; fig s1a	Cai Y, Yang L, Hu G, Chen X, Niu F, Yuan L, et al. Regulation of morphine-induced synaptic alterations: Role of oxidative stress, ER stress, and autophagy. J Cell Biol. 2016;215:245-258 pubmed
immunohistochemistry; mouse; fig 7	Hua Z, Emiliani F, Nathans J. Rac1 plays an essential role in axon growth and guidance and in neuronal survival in the central and peripheral nervous systems. Neural Dev. 2015;10:21 pubmed publisher
immunocytochemistry; rat; 1:25 immunohistochemistry; rat; 1:25	Fekete C, Chiou T, Miralles C, Harris R, Fiondella C, LoTurco J, et al. In vivo clonal overexpression of neuroligin 3 and neuroligin 2 in neurons of the rat cerebral cortex: Differential effects on GABAergic synapses and neuronal migration. J Comp Neurol. 2015;523:1359-78 pubmed publisher
	Lee S, Weltzien F, Madigan M, Martin P, GrÃ¼nert U. Identification of Aâ…¡ amacrine, displaced amacrine, and bistratified ganglion cell types in human retina with antibodies against calretinin. J Comp Neurol. 2016;524:39-53 pubmed publisher
	Gresa Arribas N, AriÃ±o H, MartÃnez HernÃ¡ndez E, Petit Pedrol M, Sabater L, Saiz A, et al. Antibodies to inhibitory synaptic proteins in neurological syndromes associated with glutamic acid decarboxylase autoimmunity. PLoS ONE. 2015;10:e0121364 pubmed publisher
	Ikeda T, Makino Y, Yamada M. 17Î±-estradiol is generated locally in the male rat brain and can regulate GAD65 expression and anxiety. Neuropharmacology. 2015;90:9-14 pubmed publisher
	Weltzien F, Percival K, Martin P, GrÃ¼nert U. Analysis of bipolar and amacrine populations in marmoset retina. J Comp Neurol. 2015;523:313-34 pubmed publisher
	Biane J, Conner J, Tuszynski M. Nerve growth factor is primarily produced by GABAergic neurons of the adult rat cortex. Front Cell Neurosci. 2014;8:220 pubmed publisher
	Vastagh C, Schwirtlich M, Kwakowsky A, Erdelyi F, Margolis F, Yanagawa Y, et al. The spatiotemporal segregation of GAD forms defines distinct GABA signaling functions in the developing mouse olfactory system and provides novel insights into the origin and migration of GnRH neurons. Dev Neurobiol. 2015;75:249-70 pubmed publisher
	Ren Z, Sahir N, Murakami S, Luellen B, Earnheart J, Lal R, et al. Defects in dendrite and spine maturation and synaptogenesis associated with an anxious-depressive-like phenotype of GABAA receptor-deficient mice. Neuropharmacology. 2015;88:171-9 pubmed publisher
	Moscato E, Peng X, Jain A, Parsons T, Dalmau J, Balice Gordon R. Acute mechanisms underlying antibody effects in anti-N-methyl-D-aspartate receptor encephalitis. Ann Neurol. 2014;76:108-19 pubmed publisher
	Campbell J, Singh D, Hollett G, Dravid S, Sailor M, Arikkath J. Spatially selective photoconductive stimulation of live neurons. Front Cell Neurosci. 2014;8:142 pubmed publisher
	El Khoury R, Panayotis N, Matagne V, Ghata A, Villard L, Roux J. GABA and glutamate pathways are spatially and developmentally affected in the brain of Mecp2-deficient mice. PLoS ONE. 2014;9:e92169 pubmed publisher
	Hannan M, Mohibbullah M, Hong Y, Nam J, Moon I. Gelidium amansii promotes dendritic spine morphology and synaptogenesis, and modulates NMDA receptor-mediated postsynaptic current. In Vitro Cell Dev Biol Anim. 2014;50:445-52 pubmed publisher
	Han K, Holder J, Schaaf C, Lu H, Chen H, Kang H, et al. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature. 2013;503:72-7 pubmed publisher
	Pribiag H, Stellwagen D. TNF-? downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors. J Neurosci. 2013;33:15879-93 pubmed publisher
	Linsalata A, Chen X, Winters C, Reese T. Electron tomography on Î³-aminobutyric acid-ergic synapses reveals a discontinuous postsynaptic network of filaments. J Comp Neurol. 2014;522:921-36 pubmed publisher
	Weltzien F, Dimarco S, Protti D, Daraio T, Martin P, Grünert U. Characterization of secretagogin-immunoreactive amacrine cells in marmoset retina. J Comp Neurol. 2014;522:435-55 pubmed publisher
	Sherry D, Blackburn B. P-Rex2, a Rac-guanine nucleotide exchange factor, is expressed selectively in ribbon synaptic terminals of the mouse retina. BMC Neurosci. 2013;14:70 pubmed publisher
	Vuong L, Brobst D, Ivanovic I, Sherry D, Al Ubaidi M. p53 selectively regulates developmental apoptosis of rod photoreceptors. PLoS ONE. 2013;8:e67381 pubmed publisher
	Takahashi H, Katayama K, Sohya K, Miyamoto H, Prasad T, Matsumoto Y, et al. Selective control of inhibitory synapse development by Slitrk3-PTP? trans-synaptic interaction. Nat Neurosci. 2012;15:389-98, S1-2 pubmed publisher
	Guirado R, Sanchez Matarredona D, Varea E, Crespo C, Blasco Ibáñez J, Nacher J. Chronic fluoxetine treatment in middle-aged rats induces changes in the expression of plasticity-related molecules and in neurogenesis. BMC Neurosci. 2012;13:5 pubmed publisher
	de Melo J, Peng G, Chen S, Blackshaw S. The Spalt family transcription factor Sall3 regulates the development of cone photoreceptors and retinal horizontal interneurons. Development. 2011;138:2325-36 pubmed publisher
	Ripley B, Otto S, Tiglio K, Williams M, Ghosh A. Regulation of synaptic stability by AMPA receptor reverse signaling. Proc Natl Acad Sci U S A. 2011;108:367-72 pubmed publisher
	Marcano Reik A, Prasad T, Weiner J, Blumberg M. An abrupt developmental shift in callosal modulation of sleep-related spindle bursts coincides with the emergence of excitatory-inhibitory balance and a reduction of somatosensory cortical plasticity. Behav Neurosci. 2010;124:600-11 pubmed publisher
	Hu S, Arnold A, Hutchens J, Radicke J, Cravatt B, Wager Miller J, et al. Architecture of cannabinoid signaling in mouse retina. J Comp Neurol. 2010;518:3848-66 pubmed publisher
	Grubb M, Burrone J. Activity-dependent relocation of the axon initial segment fine-tunes neuronal excitability. Nature. 2010;465:1070-4 pubmed publisher
	Gallagher S, Witkovsky P, Roux M, Low M, Otero Corchon V, Hentges S, et al. beta-Endorphin expression in the mouse retina. J Comp Neurol. 2010;518:3130-48 pubmed publisher
	Guan H, Maness P. Perisomatic GABAergic innervation in prefrontal cortex is regulated by ankyrin interaction with the L1 cell adhesion molecule. Cereb Cortex. 2010;20:2684-93 pubmed publisher
	Vierbuchen T, Ostermeier A, Pang Z, Kokubu Y, Sudhof T, Wernig M. Direct conversion of fibroblasts to functional neurons by defined factors. Nature. 2010;463:1035-41 pubmed publisher
	Flak J, Ostrander M, Tasker J, Herman J. Chronic stress-induced neurotransmitter plasticity in the PVN. J Comp Neurol. 2009;517:156-65 pubmed publisher
	Ghuman S, Morris R, Spiller D, Smith R, Dobson H. Integration between different hypothalamic nuclei involved in stress and GnRH secretion in the ewe. Reprod Domest Anim. 2010;45:1065-73 pubmed publisher
	Pinto M, Sousa M, Lima D, Tavares I. Participation of mu-opioid, GABA(B), and NK1 receptors of major pain control medullary areas in pathways targeting the rat spinal cord: implications for descending modulation of nociceptive transmission. J Comp Neurol. 2008;510:175-87 pubmed publisher
	Rodrigo Angulo M, Heredero S, Rodríguez Veiga E, Reinoso Suárez F. GABAergic and non-GABAergic thalamic, hypothalamic and basal forebrain projections to the ventral oral pontine reticular nucleus: their implication in REM sleep modulation. Brain Res. 2008;1210:116-25 pubmed publisher
	Chen J, Kitanishi T, Ikeda T, Matsuki N, Yamada M. Contextual learning induces an increase in the number of hippocampal CA1 neurons expressing high levels of BDNF. Neurobiol Learn Mem. 2007;88:409-15 pubmed
	Lakowski J, Majumder A, Lauderdale J. Mechanisms controlling Pax6 isoform expression in the retina have been conserved between teleosts and mammals. Dev Biol. 2007;307:498-520 pubmed
	Shibasaki K, Suzuki M, Mizuno A, Tominaga M. Effects of body temperature on neural activity in the hippocampus: regulation of resting membrane potentials by transient receptor potential vanilloid 4. J Neurosci. 2007;27:1566-75 pubmed
	Ikeda T, Tamura N, Matsuki N, Yamada M. Conserved role of brain-derived neurotrophic factor in Val66Met: target-selective reinforcement of GABAergic synapses. Neuroreport. 2006;17:1847-51 pubmed
	Vergaño Vera E, Yusta Boyo M, De Castro F, Bernad A, de Pablo F, Vicario Abejón C. Generation of GABAergic and dopaminergic interneurons from endogenous embryonic olfactory bulb precursor cells. Development. 2006;133:4367-79 pubmed
	Blurton Jones M, Tuszynski M. Estradiol-induced modulation of estrogen receptor-beta and GABA within the adult neocortex: a potential transsynaptic mechanism for estrogen modulation of BDNF. J Comp Neurol. 2006;499:603-12 pubmed
	Shibasaki K, Takebayashi H, Ikenaka K, Feng L, Gan L. Expression of the basic helix-loop-factor Olig2 in the developing retina: Olig2 as a new marker for retinal progenitors and late-born cells. Gene Expr Patterns. 2007;7:57-65 pubmed
	Jevince A, Kadison S, Pittman A, Chien C, Kaprielian Z. Distribution of EphB receptors and ephrin-B1 in the developing vertebrate spinal cord. J Comp Neurol. 2006;497:734-50 pubmed
	Crombie D, Turer M, Zuasti B, Wood B, McNaughton D, Nandakumar K, et al. Destructive effects of murine arthritogenic antibodies to type II collagen on cartilage explants in vitro. Arthritis Res Ther. 2005;7:R927-37 pubmed
	Weiner J, Wang X, Tapia J, Sanes J. Gamma protocadherins are required for synaptic development in the spinal cord. Proc Natl Acad Sci U S A. 2005;102:8-14 pubmed
	Ohba S, Ikeda T, Ikegaya Y, Nishiyama N, Matsuki N, Yamada M. BDNF locally potentiates GABAergic presynaptic machineries: target-selective circuit inhibition. Cereb Cortex. 2005;15:291-8 pubmed
	Zhang H, Webb D, Asmussen H, Horwitz A. Synapse formation is regulated by the signaling adaptor GIT1. J Cell Biol. 2003;161:131-42 pubmed
	Jimenez A, Fernández Llebrez P, Pérez Fígares J. Neural input and neural control of the subcommissural organ. Microsc Res Tech. 2001;52:520-33 pubmed
	Phelps P, Alijani A, Tran T. Ventrally located commissural neurons express the GABAergic phenotype in developing rat spinal cord. J Comp Neurol. 1999;409:285-98 pubmed
	Essrich C, Lorez M, Benson J, Fritschy J, Luscher B. Postsynaptic clustering of major GABAA receptor subtypes requires the gamma 2 subunit and gephyrin. Nat Neurosci. 1998;1:563-71 pubmed
	Crump F, Fremeau R, Craig A. Localization of the brain-specific high-affinity l-proline transporter in cultured hippocampal neurons: molecular heterogeneity of synaptic terminals. Mol Cell Neurosci. 1999;13:25-39 pubmed
	Labandeira Garcia J, Liste I, Tobio J, Rozas G, Lopez Martin E, Guerra M. Intrathalamic striatal grafts survive and affect circling behaviour in adult rats with excitotoxically lesioned striatum. Neuroscience. 1995;68:737-49 pubmed
	Hagopian W, Michelsen B, Karlsen A, Larsen F, Moody A, Grubin C, et al. Autoantibodies in IDDM primarily recognize the 65,000-M(r) rather than the 67,000-M(r) isoform of glutamic acid decarboxylase. Diabetes. 1993;42:631-6 pubmed
	Kim J, Richter W, Aanstoot H, Shi Y, Fu Q, Rajotte R, et al. Differential expression of GAD65 and GAD67 in human, rat, and mouse pancreatic islets. Diabetes. 1993;42:1799-808 pubmed
	Benson D, Watkins F, Steward O, Banker G. Characterization of GABAergic neurons in hippocampal cell cultures. J Neurocytol. 1994;23:279-95 pubmed
	Richter W, Shi Y, Baekkeskov S. Autoreactive epitopes defined by diabetes-associated human monoclonal antibodies are localized in the middle and C-terminal domains of the smaller form of glutamate decarboxylase. Proc Natl Acad Sci U S A. 1993;90:2832-6 pubmed
	Chang Y, Gottlieb D. Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase. J Neurosci. 1988;8:2123-30 pubmed
	Kaufman D, Erlander M, Clare Salzler M, Atkinson M, Maclaren N, Tobin A. Autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus. J Clin Invest. 1992;89:283-92 pubmed

product information

Internal ID :

3646

Name :

GAD-6

Depositor Name :

Gottlieb, D.I.

Depositor Institution :

Washington University School of Medicine

Date Deposited :

10/8/90

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Glutamic acid decarboxylase; GAD

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Fish,Human,Marmoset,Mouse,Rat,Zebrafish

Species Tested (legacy) :

rat, mouse, human

Initial Publication Pubmed ID :

3385490

Depositor Notes (Special Instructions) :

Immunoprecipitates as a dimer but migrates as a single band on SDS-PAGE western blot.

Collections :

Enzymes,Human,Neurodevelopment,Skeletal muscle

Search Keywords :

Gottlieb, David I., Glutamic acid decarboxylase; GAD, Rat, MIgG2a, Rat/Mouse/Human/Zebrafish, Gad2, Gad65, AB_528264, monoclonal

Antigen Molecular Weight :

apparent 59 kDa, predicted 65 kDa

Gene :

Gad2

Alternate Gene Name(s) :

Gad65

Uniprot ID :

Q05683

Antibody Registry ID :

AB_528264

Immunogen :

Purified glutamic acid decarboxylase from rat brain

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal a.a. 423-585

Epitope Map PubMed ID :

7681990

Recommended Applications :

Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

single band on Western analysis

Immunohistochemistry Pubmed IDs :

1370298 8089704 8577370 10196563 10379921 10049529 11241862 11241862 15574493 15238431 17038521 16786562 17179856 17301165 16815098 17509554 17765576 18407254 20543823 23844743 21173224 20939660 24399252 25565602 20156840 25125027 25107590 24153177

Immunofluorescence Pubmed IDs :

8243826 15238431 21173224 25147503 19602180 23982982 25446575 23852983 25262625 25565602 26053777 29670169 30401922 31005376

Western Blot Pubmed IDs :

3385490 1370298 7681990 8454115 8243826 31005376

Immunoprecipitation Pubmed IDs :

1370298 7681990 8454115 8243826

Pubmed IDs :

24089494 17179856

Depositor Growth Medium :

Iscove's

Additional Information :

This antibody has tested positive on marmoset tissues.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Neurosci. 8, 2123-2130.; J. Clin. Invest. 89, 283-292.; PNAS 90, 2832-2836.; Diabetes 42, 631-636.; Diabetes 42, 1799-1808.; J. Neurocytol. 23, 279-295.; Neurosci. 68, 737-749.; Nat. Neurosci. 1(7), 563-571.; J. Comp. Neurol. 409, 285-298.; Mol. Cell. Neurosci. 13, 25-39.; Microsc. Res. Tech. 52, 520-533.; PNAS 102(1), 8-14.; Cereb. Cortex 15, 291-298.; Development 133, 4367-4379.; J. Comp. Neurol. 497, 734-750.; Neuroreport 17(18), 1847-1851.; J. Neurosci. 27(7), 1566-1575.; Gene Expr. Patterns 7, 57-65.; Dev. Biol. 307, 498-520.; Neurobiol. Learn. Mem. 88, 409-415.; Brain Res. 1210, 116-125.; Nature 465, 1070-1074.; PNAS 108(1), 367-372.; J. Neurosci. 33(40), 15879-15893. doi: 10.1523/jneurosci.0530-13.2013.

more info or order :

Developmental Studies Hybridoma Bank product webpage