Epithelial cell adhesion molecule (murine); CD326 | Developmental Studies Hybridoma Bank G8.8 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Epithelial cell adhesion molecule (murine); CD326

catalog :

G8.8

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

G8.8

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 3g	Kim W, Khan S, Gvozdenovic Jeremic J, Kim Y, Dahlman J, Kim H, et al. Hippo signaling interactions with Wnt/?-catenin and Notch signaling repress liver tumorigenesis. J Clin Invest. 2017;127:137-152 pubmed publisher
flow cytometry; mouse; fig 1e	Sun L, Sun C, Liang Z, Li H, Chen L, Luo H, et al. FSP1(+) fibroblast subpopulation is essential for the maintenance and regeneration of medullary thymic epithelial cells. Sci Rep. 2015;5:14871 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig s12a	Manieri N, Mack M, Himmelrich M, Worthley D, Hanson E, Eckmann L, et al. Mucosally transplanted mesenchymal stem cells stimulate intestinal healing by promoting angiogenesis. J Clin Invest. 2015;125:3606-18 pubmed publisher
immunocytochemistry; mouse	Rattay K, Claude J, Rezavandy E, Matt S, Hofmann T, Kyewski B, et al. Homeodomain-interacting protein kinase 2, a novel autoimmune regulator interaction partner, modulates promiscuous gene expression in medullary thymic epithelial cells. J Immunol. 2015;194:921-8 pubmed publisher
flow cytometry; mouse; fig 2	Dewas C, Chen X, Honda T, Junttila I, Linton J, Udey M, et al. TSLP expression: analysis with a ZsGreen TSLP reporter mouse. J Immunol. 2015;194:1372-80 pubmed publisher
flow cytometry; mouse; fig s3	Ding X, Lucas T, Marcuzzi G, Pfister H, Eming S. Distinct functions of epidermal and myeloid-derived VEGF-A in skin tumorigenesis mediated by HPV8. Cancer Res. 2015;75:330-43 pubmed publisher
	Chen P, Zhang H, Sun X, Hu Y, Jiang W, Liu Z, et al. microRNA-449a modulates medullary thymic epithelial cell differentiation. Sci Rep. 2017;7:15915 pubmed publisher
	Zhao X, Deak E, Soderberg K, Linehan M, Spezzano D, Zhu J, et al. Vaginal submucosal dendritic cells, but not Langerhans cells, induce protective Th1 responses to herpes simplex virus-2. J Exp Med. 2003;197:153-62 pubmed

product information

Internal ID :

3031

Name :

G8.8

Depositor Name :

Farr, A.G .

Depositor Institution :

University of Washington

Date Deposited :

9/11/97

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Epithelial cell adhesion molecule (murine); CD326

Antigen Species :

Mouse

Host Species :

rat

Isotype :

RIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Mouse

Species Tested (legacy) :

mouse (not rat or human)

Initial Publication Pubmed ID :

2016514

Depositor Notes (Special Instructions) :

This antibody does not recognize human or rat EpCAM.

Collections :

CD antigens,Cell adhesion,Cell signaling

Search Keywords :

Farr, Andrew G. , Epithelial cell adhesion molecule (murine); CD326, Mouse, RIgG2a, Mouse, Epcam, CD326, gp40, AB_2098655, Monoclonal, Cell adhesion/Cluster Determinant antigens, Western blot/Immunohistochemistry/Immunofluorescence/Immunoprecipitation/FACS

Antigen Molecular Weight :

~40 kDa

Gene :

Epcam

Alternate Gene Name(s) :

CD326, gp40

Uniprot ID :

Q99JW5

Antibody Registry ID :

AB_2098655

Synonyms (Alternate Clone Names) :

CD326

Immunogen :

TE-71 mouse thymic epithelial cell line

Immunogen Sequence :

Full length native protein

Clonality :

Monoclonal

Myeloma Strain :

X63.Ag8.653

Epitope Mapped :

Recommended Applications :

FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

2016514 19369401 16127706 8617310 8566052 31391455

Immunofluorescence Pubmed IDs :

29162901 27869648 27815529 26245375 25627671 25314078 25024225 24465914 24086471 22872743 21854845 20046825 19097184 18832676 18172306 17328885 16107472 15840656 12538655 12151334

Western Blot Pubmed IDs :

8617310

Immunoprecipitation Pubmed IDs :

2016514

FFPE Pubmed IDs :

31391455

FACS Pubmed IDs :

2016514 26694097 25811463 24465914 22972926 22072979 18762568 17428826 17021343 17162372 16936209 16107472 15601913 15620358 14688197 12700182 8617310 8566052

Additional Information :

G8.8 has also been used to purify thymic epithelial cells (PMID: 20548034 17127749). RRID:AB_2098655

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Histochem. Cytochem. 39, 645-653.; Eur. J. Immunol. 26, 110-114.; Eur. J. Immunol. 26, 401-408.

more info or order :

Developmental Studies Hybridoma Bank product webpage