5-bromo-2'-deoxyuridine BrdU | Developmental Studies Hybridoma Bank G3G4 (anti-BrdU) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

5-bromo-2'-deoxyuridine BrdU

catalog :

G3G4 (anti-BrdU)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

G3G4 (anti-BrdU)

reactivity :

fish, mouse, chicken, zebrafish

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 43

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; zebrafish ; 1:100; loading ...; fig 1h	Thomas E, Raible D. Distinct progenitor populations mediate regeneration in the zebrafish lateral line. elife. 2019;8: pubmed publisher
immunohistochemistry - paraffin section; fish; 1:600; loading ...; fig 5c	Chauvigné F, Ollé J, Gonzalez W, Duncan N, Gimenez I, Cerdà J. Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole. PLoS ONE. 2017;12:e0174387 pubmed publisher
immunohistochemistry - paraffin section; scFv; 1:300; fig 4	Feng L, Wang Y, Cai H, Sun G, Niu W, Xin Q, et al. ADAM10-Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice. J Cell Sci. 2016;129:2202-12 pubmed publisher
immunohistochemistry; chicken; 1:100; fig 1	Nitzan E, Avraham O, Kahane N, Ofek S, Kumar D, Kalcheim C. Dynamics of BMP and Hes1/Hairy1 signaling in the dorsal neural tube underlies the transition from neural crest to definitive roof plate. BMC Biol. 2016;14:23 pubmed publisher
immunohistochemistry - paraffin section; scFv; fig 2	Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, LlÃ¨res D, et al. The cell proliferation antigen Ki-67 organises heterochromatin. elife. 2016;5:e13722 pubmed publisher
immunohistochemistry - frozen section; scFv; fig s2	Valenzuela N, Fan Q, Fa ak F, Soibam B, Nagandla H, Liu Y, et al. Cardiomyocyte-specific conditional knockout of the histone chaperone HIRA in mice results in hypertrophy, sarcolemmal damage and focal replacement fibrosis. Dis Model Mech. 2016;9:335-45 pubmed publisher
immunohistochemistry; scFv; 1:2000	Rivera P, Bindila L, Pastor A, PÃ©rez MartÃn M, PavÃ³n F, Serrano A, et al. Pharmacological blockade of the fatty acid amide hydrolase (FAAH) alters neural proliferation, apoptosis and gliosis in the rat hippocampus, hypothalamus and striatum in a negative energy context. Front Cell Neurosci. 2015;9:98 pubmed publisher
flow cytometry; mouse; fig 1	Boo K, Bhin J, Jeon Y, Kim J, Shin H, Park J, et al. Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells. Nat Commun. 2015;6:6810 pubmed publisher
immunohistochemistry; scFv; fig 7	Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, et al. Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity. Autophagy. 2014;10:2036-52 pubmed publisher
immunocytochemistry; scFv; 1:1000	Vergaño Vera E, Diaz Guerra E, Rodríguez Traver E, Méndez Gómez H, Solis O, Pignatelli J, et al. Nurr1 blocks the mitogenic effect of FGF-2 and EGF, inducing olfactory bulb neural stem cells to adopt dopaminergic and dopaminergic-GABAergic neuronal phenotypes. Dev Neurobiol. 2015;75:823-41 pubmed publisher
immunocytochemistry; zebrafish ; 1:100; fig 7	Tapanes Castillo A, Shabazz F, Mboge M, Vajn K, Oudega M, Plunkett J. Characterization of a novel primary culture system of adult zebrafish brainstem cells. J Neurosci Methods. 2014;223:11-9 pubmed publisher
	Alibardi L. Wnt-1 immunodetection in the regenerating tail of lizard suggests it is involved in the proliferation and distal growth of the blastema. Acta Histochem. 2017;119:211-219 pubmed publisher
	Choubey P, Roy J. Rab11, a vesicular trafficking protein, affects endoreplication through Ras-mediated pathway in Drosophila melanogaster. Cell Tissue Res. 2017;367:269-282 pubmed publisher
	Lough D, Chambers C, Germann G, Bueno R, Reichensperger J, Swanson E, et al. Regulation of ADSC Osteoinductive Potential Using Notch Pathway Inhibition and Gene Rescue: A Potential On/Off Switch for Clinical Applications in Bone Formation and Reconstructive Efforts. Plast Reconstr Surg. 2016;138:642e-52e pubmed publisher
	Nechiporuk T, MCGANN J, Mullendorff K, Hsieh J, Wurst W, Floss T, et al. The REST remodeling complex protects genomic integrity during embryonic neurogenesis. elife. 2016;5:e09584 pubmed publisher
	Alibardi L. Immunolocalization indicates that both original and regenerated lizard tail tissues contain populations of long retaining cells, putative stem/progenitor cells. Microsc Res Tech. 2015;78:1032-45 pubmed publisher
	Gupte T. Mitochondrial Fragmentation Due to Inhibition of Fusion Increases Cyclin B through Mitochondrial Superoxide Radicals. PLoS ONE. 2015;10:e0126829 pubmed publisher
	Wen J, Yeo S, Wang C, Chen S, Sun S, Haas M, et al. Autophagy inhibition re-sensitizes pulse stimulation-selected paclitaxel-resistant triple negative breast cancer cells to chemotherapy-induced apoptosis. Breast Cancer Res Treat. 2015;149:619-29 pubmed publisher
	Wang J, Cheng A, Wakade C, Yu R. Ganglioside GD3 is required for neurogenesis and long-term maintenance of neural stem cells in the postnatal mouse brain. J Neurosci. 2014;34:13790-800 pubmed publisher
	Liang D, Wang X, Mittal A, Dhiman S, Hou S, Degenhardt K, et al. Mesodermal expression of integrin Î±5Î²1 regulates neural crest development and cardiovascular morphogenesis. Dev Biol. 2014;395:232-44 pubmed publisher
	Umazume K, Tsukahara R, Liu L, Fernandez de Castro J, McDonald K, Kaplan H, et al. Role of retinal pigment epithelial cell ?-catenin signaling in experimental proliferative vitreoretinopathy. Am J Pathol. 2014;184:1419-28 pubmed publisher
	Rivera P, Perez Martin M, Pavón F, Serrano A, Crespillo A, Cifuentes M, et al. Pharmacological administration of the isoflavone daidzein enhances cell proliferation and reduces high fat diet-induced apoptosis and gliosis in the rat hippocampus. PLoS ONE. 2013;8:e64750 pubmed publisher
	Chavarría T, Baleriola J, Mayordomo R, de Pablo F, de la Rosa E. Early neural cell death is an extensive, dynamic process in the embryonic chick and mouse retina. ScientificWorldJournal. 2013;2013:627240 pubmed publisher
	Ma S, Kwon H, Huang Z. Ric-8a, a guanine nucleotide exchange factor for heterotrimeric G proteins, regulates bergmann glia-basement membrane adhesion during cerebellar foliation. J Neurosci. 2012;32:14979-93 pubmed publisher
	Ma W, Coon S, Zhao L, Fariss R, Wong W. A2E accumulation influences retinal microglial activation and complement regulation. Neurobiol Aging. 2013;34:943-60 pubmed publisher
	Hara M, Tabata K, Suzuki T, Do M, Mizunoya W, Nakamura M, et al. Calcium influx through a possible coupling of cation channels impacts skeletal muscle satellite cell activation in response to mechanical stretch. Am J Physiol Cell Physiol. 2012;302:C1741-50 pubmed publisher
	Stuck M, Conley S, Naash M. Defects in the outer limiting membrane are associated with rosette development in the Nrl-/- retina. PLoS ONE. 2012;7:e32484 pubmed publisher
	Veverytsa L, Allan D. Temporally tuned neuronal differentiation supports the functional remodeling of a neuronal network in Drosophila. Proc Natl Acad Sci U S A. 2012;109:E748-56 pubmed publisher
	Sugimoto C, Morita S, Miyata S. Overexpression of IgLON cell adhesion molecules changes proliferation and cell size of cortical astrocytes. Cell Biochem Funct. 2012;30:400-5 pubmed publisher
	Gerbe F, van Es J, Makrini L, Brulin B, Mellitzer G, Robine S, et al. Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium. J Cell Biol. 2011;192:767-80 pubmed publisher
	MARTINEZ DE LUNA R, Kelly L, El Hodiri H. The Retinal Homeobox (Rx) gene is necessary for retinal regeneration. Dev Biol. 2011;353:10-8 pubmed publisher
	Tamiya S, Liu L, Kaplan H. Epithelial-mesenchymal transition and proliferation of retinal pigment epithelial cells initiated upon loss of cell-cell contact. Invest Ophthalmol Vis Sci. 2010;51:2755-63 pubmed publisher
	Vergaño Vera E, Yusta Boyo M, De Castro F, Bernad A, de Pablo F, Vicario Abejón C. Generation of GABAergic and dopaminergic interneurons from endogenous embryonic olfactory bulb precursor cells. Development. 2006;133:4367-79 pubmed
	Shibasaki K, Takebayashi H, Ikenaka K, Feng L, Gan L. Expression of the basic helix-loop-factor Olig2 in the developing retina: Olig2 as a new marker for retinal progenitors and late-born cells. Gene Expr Patterns. 2007;7:57-65 pubmed
	Garelli A, Rotstein N, Politi L. Docosahexaenoic acid promotes photoreceptor differentiation without altering Crx expression. Invest Ophthalmol Vis Sci. 2006;47:3017-27 pubmed
	Francisco Morcillo J, Hidalgo Sanchez M, Martin Partido G. Spatial and temporal patterns of proliferation and differentiation in the developing turtle eye. Brain Res. 2006;1103:32-48 pubmed
	Otaegi G, Yusta Boyo M, Vergaño Vera E, Méndez Gómez H, Carrera A, Abad J, et al. Modulation of the PI 3-kinase-Akt signalling pathway by IGF-I and PTEN regulates the differentiation of neural stem/precursor cells. J Cell Sci. 2006;119:2739-48 pubmed
	Yusta Boyo M, Gonzalez M, Pavon N, Martin A, de la Fuente R, Garcia Castro J, et al. Absence of hematopoiesis from transplanted olfactory bulb neural stem cells. Eur J Neurosci. 2004;19:505-12 pubmed
	Vicario Abejón C, Yusta Boyo M, Fernández Moreno C, de Pablo F. Locally born olfactory bulb stem cells proliferate in response to insulin-related factors and require endogenous insulin-like growth factor-I for differentiation into neurons and glia. J Neurosci. 2003;23:895-906 pubmed
	Dominov J, Dunn J, Miller J. Bcl-2 expression identifies an early stage of myogenesis and promotes clonal expansion of muscle cells. J Cell Biol. 1998;142:537-44 pubmed
	George Weinstein M, Foster R, Gerhart J, Kaufman S. In vitro and in vivo expression of alpha 7 integrin and desmin define the primary and secondary myogenic lineages. Dev Biol. 1993;156:209-29 pubmed
	Foster R, Thompson J, Kaufman S. A laminin substrate promotes myogenesis in rat skeletal muscle cultures: analysis of replication and development using antidesmin and anti-BrdUrd monoclonal antibodies. Dev Biol. 1987;122:11-20 pubmed
	Hayashi Y, Shiraishi T, Tani J, Araki K, Kurashige T. [Pituitary reserve of growth hormone in adolescent patients with cerebral palsy and mental retardation]. No To Hattatsu. 1989;21:239-44 pubmed

product information

Internal ID :

2815

Name :

G3G4 (anti-BrdU)

Depositor Name :

Kaufman, S.J.

Depositor Institution :

University of Illinois

Date Deposited :

10/5/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

5-bromo-2'-deoxyuridine BrdU

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

All species labeled with BrdU

Species Tested (legacy) :

all

Initial Publication Pubmed ID :

3297850

Depositor Notes (Special Instructions) :

This antibody detects BrdU incorporated into cell nuclei during S-phase. 2N HCL treatment of nuclei increases antibody access to incorporated BrdU within the DNA (see Foster et al., 1987 for details).

Collections :

C. elegans,Cell markers,Drosophila antigens

Search Keywords :

Kaufman, Stephen J., 5-bromo-2'-deoxyuridine (BrdUrd); Proliferation Marker, Not applicable, MIgG1, kappa light chain, All species labeled with BrdU, , , AB_2314035, monoclonal, drosophila

Gene :

BrdU

Antibody Registry ID :

AB_2314035

Synonyms (Alternate Clone Names) :

G3G4(AntiBrdUrd), Bromodeoxyuridine

Immunogen :

5-bromo-2'-deoxyuridine-BSA

Clonality :

Monoclonal

Myeloma Strain :

Sp 2/0 Ag 14

Epitope Mapped :

Recommended Applications :

FACS,FFPE,Immunofluorescence,Immunohistochemistry

Immunohistochemistry Pubmed IDs :

21334323 20042656 16815098 17038521 16797493 16799048 16787946 12574418 8449367 21334323 24316294 23100420 22374746 25297105 23741384 22393011 24656918 2736123 27673535 2641562926415629 31399573 28233575

Immunofluorescence Pubmed IDs :

21383077 17038521 16787946 14984401 9679150 23710143 22427845 22460715 22819137 27677270 28233575 30396951 30377270 30866939

FFPE Pubmed IDs :

21383077 25242040 28233575

FACS Pubmed IDs :

26000631

Pubmed IDs :

25638397

Depositor Growth Medium :

Iscove's

Additional Information :

IHC paper: Acta zoologica doi: 10.1111/azo.12114

DSHB Growth Medium :

Iscove's

References (legacy) :

Dev. Biol. 156, 209-229.; J. Cell Biol. 142, 537-544.; Reprod. Tox. 13(1), 45-52.; J. Neurosci. 23(3), 895-906.; Eur. J. Neurosci. 19, 505-512.; Invest. Ophthalmol. Vis. Sci. 47(7), 3017-3027.; J. Cell Sci. 119, 2739-2748.; Development 133, 4367-4379.; Gene Expr. Patterns 7, 57-65.; Brain Res. 1103, 32-48.; Gene Expr. Patterns 7, 57-65.; Invest. Ophthalmol. Vis. Sci. 51(5), 2755-2763.; J. Cell Biol. 192(5), 767-780.; Dev. Biol. 353, 10-18.; doi: 10.1007/s10549-015-3283-9.

more info or order :

Developmental Studies Hybridoma Bank product webpage