Flamingo (starry night) | Developmental Studies Hybridoma Bank Flamingo #74 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Flamingo (starry night)

catalog :

Flamingo #74

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Flamingo #74

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Reference
Ressurreição M, Warrington S, Strutt D. Rapid Disruption of Dishevelled Activity Uncovers an Intercellular Role in Maintenance of Prickle in Core Planar Polarity Protein Complexes. Cell Rep. 2018;25:1415-1424.e6 pubmed publisher
Wang Y, Yan J, Lee H, Lu Q, Adler P. The proteins encoded by the Drosophila Planar Polarity Effector genes inturned, fuzzy and fritz interact physically and can re-pattern the accumulation of "upstream" Planar Cell Polarity proteins. Dev Biol. 2014;394:156-69 pubmed publisher
Stronach B. Extensive nonmuscle expression and epithelial apicobasal localization of the Drosophila ALP/Enigma family protein, Zasp52. Gene Expr Patterns. 2014;15:67-79 pubmed publisher
Thomas C, Strutt D. Rabaptin-5 and Rabex-5 are neoplastic tumour suppressor genes that interact to modulate Rab5 dynamics in Drosophila melanogaster. Dev Biol. 2014;385:107-21 pubmed publisher
Matis M, Axelrod J, Galic M. A universal analysis tool for the detection of asymmetric signal distribution in microscopic images. Dev Dyn. 2012;241:1301-9 pubmed publisher
Mouri K, Horiuchi S, Uemura T. Cohesin controls planar cell polarity by regulating the level of the seven-pass transmembrane cadherin Flamingo. Genes Cells. 2012;17:509-24 pubmed publisher
Tanaka K, Barmina O, Kopp A. Distinct developmental mechanisms underlie the evolutionary diversification of Drosophila sex combs. Proc Natl Acad Sci U S A. 2009;106:4764-9 pubmed publisher
Steinel M, Whitington P. The atypical cadherin Flamingo is required for sensory axon advance beyond intermediate target cells. Dev Biol. 2009;327:447-57 pubmed publisher
Petrovic M, Hummel T. Temporal identity in axonal target layer recognition. Nature. 2008;456:800-3 pubmed publisher
Chen P, Clandinin T. The cadherin Flamingo mediates level-dependent interactions that guide photoreceptor target choice in Drosophila. Neuron. 2008;58:26-33 pubmed publisher
Sato M, Umetsu D, Murakami S, Yasugi T, Tabata T. DWnt4 regulates the dorsoventral specificity of retinal projections in the Drosophila melanogaster visual system. Nat Neurosci. 2006;9:67-75 pubmed
Usui T, Shima Y, Shimada Y, Hirano S, Burgess R, Schwarz T, et al. Flamingo, a seven-pass transmembrane cadherin, regulates planar cell polarity under the control of Frizzled. Cell. 1999;98:585-95 pubmed

product information

Internal ID :

892

Name :

Flamingo #74

Depositor Name :

Uemura, T.

Depositor Institution :

Kyoto Univ. Graduate Sch.of Biostudies

Date Deposited :

1/7/04

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Flamingo (starry night)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

10490098

Depositor Notes (Special Instructions) :

Flamingo #74 does not work in IP. Another mouse monoclonal antibody to Flamingo #71 is appropriate for IP experiments. About requests of #71, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). In immunostaining pupal wings, we fix tissues with 4% paraformaldehyde (or 3.7% formaldehyde) in PBS on ice overnight (at least for 2 hours), and incubate tissues in the mAb diluted 1:10 in PBT containing 2% BSA. We do not prefer any fix solutions including EDTA or EGTA. This is because classic cadherins are degraded under Ca++- free conditions, although we have not studied how unstable Fmi is under that condition. A portion of the Fmi ectodomain (amino acid residues 539-1219) was used as immunogen.

Collections :

Cell adhesion,Cell signaling,Drosophila antigens

Search Keywords :

Uemura, T., Flamingo (starry night), Drosophila, MIgG1, Drosophila, stan, CG11895, fmi, flam, AB_2619583, monoclonal, Cell adhesion/Cell signaling/Drosophila antigens, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 394 kDa

Gene :

stan

Alternate Gene Name(s) :

CG11895, fmi, flam

Uniprot ID :

Q9V5N8

Antibody Registry ID :

AB_2619583

Immunogen :

Extracellular domain, aa 539-1219.

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3-X63-Ag8-U1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Extracellular domain, aa 539-1219 (see depositor notes).

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

19146847 31409231

Immunofluorescence Pubmed IDs :

19146847 22563761 22689329 16369482 18400160 24104056 18978776 19255422 25072625 24846727

Depositor Growth Medium :

RPMI 1640 10% FCS, Pen/Strep & Fungizone

Additional Information :

Flamingo is the protocadherin-like wing polarity protein stan.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 98, 585-595.

more info or order :

Developmental Studies Hybridoma Bank product webpage