product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Embryonal carcinoma cell surface carbohydrate
catalog :
FE-A5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FE-A5
application :
immunohistochemistry, immunocytochemistry
more info or order :
citations: 8
| Reference |
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Bertke A, Patel A, Imai Y, Apakupakul K, Margolis T, Krause P. Latency-associated transcript (LAT) exon 1 controls herpes simplex virus species-specific phenotypes: reactivation in the guinea pig genital model and neuron subtype-specific latent expression of LAT. J Virol. 2009;83:10007-15 pubmed publisher
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Labrakakis C, Tong C, Weissman T, Torsney C, Macdermott A. Localization and function of ATP and GABAA receptors expressed by nociceptors and other postnatal sensory neurons in rat. J Physiol. 2003;549:131-42 pubmed
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Zhu Z, Kojima N, Stroud M, Hakomori S, Fenderson B. Monoclonal antibody directed to Le(y) oligosaccharide inhibits implantation in the mouse. Biol Reprod. 1995;52:903-12 pubmed
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Fenderson B, Hahnel A, Eddy E. Immunohistochemical localization of two monoclonal antibody-defined carbohydrate antigens during early murine embryogenesis. Dev Biol. 1983;100:318-27 pubmed
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Fenderson B, O Brien D, Millette C, Eddy E. Stage-specific expression of three cell surface carbohydrate antigens during murine spermatogenesis detected with monoclonal antibodies. Dev Biol. 1984;103:117-28 pubmed
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Fenderson B, Nichols E, Clausen H, Hakomori S. A monoclonal antibody defining a binary N-acetyllactosaminyl structure in lactoisooctaosylceramide (IV6Gal beta 1----4GlcNAcnLc6): a useful probe for determining differential glycosylation patterns between normal and transformed human fibroblasts. Mol Immunol. 1986;23:747-54 pubmed
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product information
Internal ID :
3049
Name :
FE-A5
Depositor Name :
Fenderson, B. / Eddy, M.
Depositor Institution :
Thomas Jefferson University
Date Deposited :
9/12/90
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Embryonal carcinoma cell surface carbohydrate
Antigen Species :
Rat
Host Species :
mouse
Isotype :
MIgM
Isotype for catalog (legacy) :
IgM
Positive Tested Species Reactivity :
Fish,Mammal,Mouse,Ovine,Rat
Species Tested (legacy) :
rat and mouse
Initial Publication Pubmed ID :
6653876
Depositor Notes (Special Instructions) :
This antibody recognizes a carbohydrate epitope found on glycolipids and glycoproteins: Gal b 1-4 G1cNAc b 1-3 R. This carbohydrate moiety is found on the surface of mouse NULLI-SCC1 and F9 testis embryonal carcinoma cells. Bind of FE-A5 is strongly inhibited by the presence of N-acetyllactoseamine, lactose and D-Galactose.
Collections :
Cell markers
Search Keywords :
Fenderson, Bruce A., Embryonal carcinoma cell surface carbohydrate, Rat, MIgM, Rat/Mouse/Fish/Ovine/Mammals, , , AB_531818, monoclonal
Antibody Registry ID :
AB_531818
Immunogen :
Rat testicular cell lysate
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
Yes
Epitope Location or Sequence :
Carbohydrate moiety Gal b 1-4 G1cNAc b 1-3 R
Epitope Map PubMed ID :
6653876
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry
Immunohistochemistry Pubmed IDs :
6653876 12665615
Immunofluorescence Pubmed IDs :
6653876 6370756 23300908 21507969 19641003
Western Blot Pubmed IDs :
6370756 7780013
ELISA Pubmed IDs :
3796623 6653876
FFPE Pubmed IDs :
6653876
Depositor Growth Medium :
RPMI 1640 10% FCS, Pen/Strep & Fungizone
Additional Information :
FE-A5 has been successfully used in TLC overlay applications (PMID: 26405105).
DSHB Growth Medium :
RPMI
References (legacy) :
Dev. Biol. 100, 318-327.; Dev. Biol. 103, 117-128.; Molec. Immun. 23, 747-754.
more info or order :
company information
Developmental Studies Hybridoma Bank