product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
fasciclin I (Drosophila)
catalog :
F5H7 anti-Fasciclin I
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F5H7 anti-Fasciclin I
reactivity :
fruit fly
application :
western blot, ELISA, immunohistochemistry
citations: 5
Reference
Farzan S, Stegman M, Ogden S, Ascano M, Black K, Tacchelly O, et al. A quantification of pathway components supports a novel model of Hedgehog signal transduction. J Biol Chem. 2009;284:28874-84 pubmed publisher
Hoehne M, de Couet H, Stuermer C, Fischbach K. Loss- and gain-of-function analysis of the lipid raft proteins Reggie/Flotillin in Drosophila: they are posttranslationally regulated, and misexpression interferes with wing and eye development. Mol Cell Neurosci. 2005;30:326-38 pubmed
Islam R, Kristiansen L, Romani S, Garcia Alonso L, Hortsch M. Activation of EGF receptor kinase by L1-mediated homophilic cell interactions. Mol Biol Cell. 2004;15:2003-12 pubmed
Stegman M, Goetz J, Ascano M, Ogden S, Nybakken K, Robbins D. The Kinesin-related protein Costal2 associates with membranes in a Hedgehog-sensitive, Smoothened-independent manner. J Biol Chem. 2004;279:7064-71 pubmed
Hortsch M, Goodman C. Drosophila fasciclin I, a neural cell adhesion molecule, has a phosphatidylinositol lipid membrane anchor that is developmentally regulated. J Biol Chem. 1990;265:15104-9 pubmed
product information
Internal ID :
3640
Name :
F5H7 anti-Fasciclin I
Depositor Name :
Goodman, C.
Depositor Institution :
University of California, Berkeley
Date Deposited :
2/1/94
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
fasciclin I (Drosophila)
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Initial Publication Pubmed ID :
2394715
Depositor Notes (Special Instructions) :
It is important to use a low concentration of saponin (0.02%) instead of triton for immunohisto- chemistry.
Collections :
Cell adhesion,Cell markers,Cell signaling,Drosophila antigens,Neurodevelopment
Search Keywords :
Goodman, C., fasciclin I (Drosophila), Drosophila, MIgG1, kappa light chain, Drosophila, Fas1, FasI; CG6588, AB_528233, Monoclonal, Drosophila antigens/Cell markers/Neurodevelopment/Cell adhesion, Immunohistochemistry/Western Blot/ELISA
Antigen Molecular Weight :
Prediced: 73 kDa
Gene :
Fas1
Alternate Gene Name(s) :
FasI; CG6588
Uniprot ID :
P10674
Antibody Registry ID :
AB_528233
Immunogen :
recombinant, protein A fusion protein
Immunogen Sequence :
Total protein
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
No
Recommended Applications :
ELISA,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Western Blot Pubmed IDs :
2394715 14718570 16154361 14645371 19717563
ELISA Pubmed IDs :
2394715
DSHB Growth Medium :
Iscove's
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US