product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Myosin heavy chain (all fast isoforms)
catalog :
F59
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F59
reactivity :
human, mouse, rat, dog, chicken, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 42
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; zebrafish ; fig 1c
Raices M, Bukata L, Sakuma S, Borlido J, Hernandez L, Hart D, et al. Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex. Dev Cell. 2017;41:540-554.e7 pubmed publisher
  • immunohistochemistry; zebrafish ; fig 2
He Q, Liu K, Tian Z, Du S. The Effects of Hsp90α1 Mutations on Myosin Thick Filament Organization. PLoS ONE. 2015;10:e0142573 pubmed publisher
  • immunohistochemistry; mouse; loading ...
Lo H, Nixon S, Hall T, Cowling B, Ferguson C, Morgan G, et al. The caveolin-cavin system plays a conserved and critical role in mechanoprotection of skeletal muscle. J Cell Biol. 2015;210:833-49 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:10
Ruparelia A, Oorschot V, Vaz R, Ramm G, Bryson Richardson R. Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency. Acta Neuropathol. 2014;128:821-33 pubmed publisher
  • immunocytochemistry; mouse; fig 9
White J, Barro M, Makarenkova H, Sanger J, Sanger J. Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes. Anat Rec (Hoboken). 2014;297:1571-84 pubmed publisher
Vieira N, Spinazzola J, Alexander M, Moreira Y, Kawahara G, Gibbs D, et al. Repression of phosphatidylinositol transfer protein ? ameliorates the pathology of Duchenne muscular dystrophy. Proc Natl Acad Sci U S A. 2017;114:6080-6085 pubmed publisher
Ruf Zamojski F, Trivedi V, Fraser S, Trinh L. Spatio-Temporal Differences in Dystrophin Dynamics at mRNA and Protein Levels Revealed by a Novel FlipTrap Line. PLoS ONE. 2015;10:e0128944 pubmed publisher
Peng W, Lee Y, Chau Y, Lu K, Kung H. Short-term exposure of zebrafish embryos to arecoline leads to retarded growth, motor impairment, and somite muscle fiber changes. Zebrafish. 2015;12:58-70 pubmed publisher
Wu H, Fong T, Chen S, Wei J, Wang I, Wen C, et al. Perturbation of cytosolic calcium by 2-aminoethoxydiphenyl borate and caffeine affects zebrafish myofibril alignment. J Appl Toxicol. 2015;35:287-94 pubmed publisher
Tu C, Tsao K, Lee S, Yang R. SCUBE3 (signal peptide-CUB-EGF domain-containing protein 3) modulates fibroblast growth factor signaling during fast muscle development. J Biol Chem. 2014;289:18928-42 pubmed publisher
Chuang H, Hsiao K, Chang H, Wu C, Pan H. The homeobox transcription factor Irxl1 negatively regulates MyoD expression and myoblast differentiation. FEBS J. 2014;281:2990-3003 pubmed publisher
Chen X, Wang Y, Wen C, Chen Y. Overdose of D-serine Induces Movement Disorder and Neuromuscular Changes of Zebrafish Larvae. J Toxicol Pathol. 2014;27:19-24 pubmed publisher
Himeda C, Debarnot C, Homma S, Beermann M, Miller J, Jones P, et al. Myogenic enhancers regulate expression of the facioscapulohumeral muscular dystrophy-associated DUX4 gene. Mol Cell Biol. 2014;34:1942-55 pubmed publisher
van Impel A, Zhao Z, Hermkens D, Roukens M, Fischer J, Peterson Maduro J, et al. Divergence of zebrafish and mouse lymphatic cell fate specification pathways. Development. 2014;141:1228-38 pubmed publisher
Bouldin C, Snelson C, Farr G, Kimelman D. Restricted expression of cdc25a in the tailbud is essential for formation of the zebrafish posterior body. Genes Dev. 2014;28:384-95 pubmed publisher
Lee Y, Fong T, Chen H, Chang C, Wang Y, Chern C, et al. Toxicity assessments of chalcone and some synthetic chalcone analogues in a zebrafish model. Molecules. 2014;19:641-50 pubmed publisher
Ikeda D, Ono Y, Hirano S, Kan no N, Watabe S. Lampreys have a single gene cluster for the fast skeletal myosin heavy chain gene family. PLoS ONE. 2013;8:e85500 pubmed publisher
Yoon S, Stadler G, Beermann M, Schmidt E, Windelborn J, Schneiderat P, et al. Immortalized myogenic cells from congenital muscular dystrophy type1A patients recapitulate aberrant caspase activation in pathogenesis: a new tool for MDC1A research. Skelet Muscle. 2013;3:28 pubmed publisher
Wang X, Zhao Z, Müller J, Iyu A, Khng A, Guccione E, et al. Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish. Biol Open. 2013;2:1203-13 pubmed publisher
Li H, Zhong Y, Wang Z, Gao J, Xu J, Chu W, et al. Smyd1b is required for skeletal and cardiac muscle function in zebrafish. Mol Biol Cell. 2013;24:3511-21 pubmed publisher
Wang J, Karra R, Dickson A, Poss K. Fibronectin is deposited by injury-activated epicardial cells and is necessary for zebrafish heart regeneration. Dev Biol. 2013;382:427-35 pubmed publisher
Ravenscroft G, Miyatake S, Lehtokari V, Todd E, Vornanen P, Yau K, et al. Mutations in KLHL40 are a frequent cause of severe autosomal-recessive nemaline myopathy. Am J Hum Genet. 2013;93:6-18 pubmed publisher
Shen M, Ozacar A, Osgood M, Boeras C, Pink J, THOMAS J, et al. Heat-shock-mediated conditional regulation of hedgehog/gli signaling in zebrafish. Dev Dyn. 2013;242:539-49 pubmed publisher
Choi W, Gemberling M, Wang J, Holdway J, Shen M, Karlstrom R, et al. In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration. Development. 2013;140:660-6 pubmed publisher
Mitsuhashi H, Mitsuhashi S, Lynn Jones T, Kawahara G, Kunkel L. Expression of DUX4 in zebrafish development recapitulates facioscapulohumeral muscular dystrophy. Hum Mol Genet. 2013;22:568-77 pubmed publisher
Johnson J, Hall T, Dyson J, Sonntag C, Ayers K, Berger S, et al. Scube activity is necessary for Hedgehog signal transduction in vivo. Dev Biol. 2012;368:193-202 pubmed publisher
de Soysa T, Ulrich A, Friedrich T, Pite D, Compton S, Ok D, et al. Macondo crude oil from the Deepwater Horizon oil spill disrupts specific developmental processes during zebrafish embryogenesis. BMC Biol. 2012;10:40 pubmed publisher
Baas D, Caussanel Boude S, Guiraud A, Calhabeu F, Delaune E, Pilot F, et al. CKIP-1 regulates mammalian and zebrafish myoblast fusion. J Cell Sci. 2012;125:3790-800 pubmed publisher
Banfi S, Monti L, Acquati F, Tettamanti G, de Eguileor M, Grimaldi A. Muscle development and differentiation in the urodele Ambystoma mexicanum. Dev Growth Differ. 2012;54:489-502 pubmed publisher
Khan T, Benaich N, Malone C, Bernardos R, Russell A, Downes G, et al. Vincristine and bortezomib cause axon outgrowth and behavioral defects in larval zebrafish. J Peripher Nerv Syst. 2012;17:76-89 pubmed publisher
Otten C, van der Ven P, Lewrenz I, Paul S, Steinhagen A, Busch Nentwich E, et al. Xirp proteins mark injured skeletal muscle in zebrafish. PLoS ONE. 2012;7:e31041 pubmed publisher
Kirchmaier B, Poon K, Schwerte T, Huisken J, Winkler C, Jungblut B, et al. The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development. Dev Biol. 2012;363:438-50 pubmed publisher
Sakowski S, Lunn J, Busta A, Palmer M, Dowling J, Feldman E. A novel approach to study motor neurons from zebrafish embryos and larvae in culture. J Neurosci Methods. 2012;205:277-82 pubmed publisher
Etard C, Roostalu U, Strahle U. Shuttling of the chaperones Unc45b and Hsp90a between the A band and the Z line of the myofibril. J Cell Biol. 2008;180:1163-75 pubmed publisher
Hanai J, Cao P, Tanksale P, Imamura S, Koshimizu E, Zhao J, et al. The muscle-specific ubiquitin ligase atrogin-1/MAFbx mediates statin-induced muscle toxicity. J Clin Invest. 2007;117:3940-51 pubmed
Wohlgemuth S, Crawford B, Pilgrim D. The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish. Dev Biol. 2007;303:483-92 pubmed
Bassermann F. [Pathogenetic and legal insurance problems in the demonstration of atypical mycobacteria as cause of occupational diseases]. Prax Pneumol. 1972;26:677-87 pubmed
Miller J, Crow M, Stockdale F. Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures. J Cell Biol. 1985;101:1643-50 pubmed
Iancu T, Lerner A, Shiloh H. Intestinal mucosa in nephropathic cystinosis. J Pediatr Gastroenterol Nutr. 1987;6:359-64 pubmed
Chu D, Klymkowsky M. The appearance of acetylated alpha-tubulin during early development and cellular differentiation in Xenopus. Dev Biol. 1989;136:104-17 pubmed
Miller J, Teal S, Stockdale F. Evolutionarily conserved sequences of striated muscle myosin heavy chain isoforms. Epitope mapping by cDNA expression. J Biol Chem. 1989;264:13122-30 pubmed
Matthews W, Jenkins R, Gonyea W. Myosin isozyme expression in response to stretch-induced hypertrophy in the Japanese quail. Anat Rec. 1990;228:255-61 pubmed
product information
Internal ID :
352
Name :
F59
Depositor Name :
Stockdale, F.E.
Depositor Institution :
Stanford University Medical Center
Date Deposited :
2/12/04
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Myosin heavy chain (all fast isoforms)
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Amphibian,Avian,Canine,Fish,Human,Mouse,Quail,Rabbit,Rat,Shark,Turtle,Xenopus,Zebrafish
Species Tested (legacy) :
chicken, quail, zebrafish, mouse, rat, frog, shark, rabbit, human
Initial Publication Pubmed ID :
3902852
Depositor Notes (Special Instructions) :
This antibody is specific for isoforms of myosin heavy chain associated with fast contracting muscle present in avian and mammalian heart.
Collections :
Cardiac development,Cytoskeleton,Human,Muscular dystrophy,Skeletal muscle
Search Keywords :
Stockdale, Frank E., Myosin heavy chain (all fast isoforms), Chicken, MIgG1, kappa light chain, Avian/Mouse/Rat/Shark/Rabbit/Human/Turtle/Fish/Amphibian/Canine, MYH1A, MYH1, MYH3, AB_528373, monoclonal
Antigen Molecular Weight :
~223 kDa
Gene :
MYH1A
Alternate Gene Name(s) :
MYH1, MYH3
Uniprot ID :
Q91352
Antibody Registry ID :
AB_528373
Immunogen :
Myosin isolated from pectoralis major
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
P3/NS1.1Ag4-1
Epitope Mapped :
Yes
Epitope Location or Sequence :
a.a. 200-250
Epitope Map PubMed ID :
2473986
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
3902852 24791063 24402197 24849601 24814716 25549301 23441066 24376886 22519643 22462669 23108159 25186829 23746549 3430244 25125171 2260780 2680681 29754802 31365871
Immunofluorescence Pubmed IDs :
3902852 22553210 24314268 22609552 24068325 22285259 22290329 22355335 17992259 23988577 24636994 24478331 23293297 22559716 24523456 4647604 17189627 27141287 16249237
Western Blot Pubmed IDs :
3902852 2473986 24814716 22519643 23746549
Immunoprecipitation Pubmed IDs :
2473986
Depositor Growth Medium :
Iscove's
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Biol. 101, 1643-1650.; Dev. Biol. 113, 238-254.; J. Biol. Chem. 264, 13122-13130.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US