product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Homeobox protein Nkx-6.1; Nkx6.1
catalog :
F55A12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F55A12
reactivity :
human, mouse, rat, bovine, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 30
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:200; loading ...; fig s6m
Yung T, Poon F, Liang M, Coquenlorge S, McGaugh E, Hui C, et al. Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals. Nat Commun. 2019;10:4647 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:50; loading ...; fig e1a
  • flow cytometry; human; 1:50; loading ...; fig 1a, s3c
Veres A, Faust A, Bushnell H, Engquist E, Kenty J, Harb G, et al. Charting cellular identity during human in vitro β-cell differentiation. Nature. 2019;569:368-373 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s6j
Sharon N, Chawla R, Mueller J, Vanderhooft J, Whitehorn L, Rosenthal B, et al. A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets. Cell. 2019;176:790-804.e13 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 3c
Bartolomé A, Zhu C, Sussel L, Pajvani U. Notch signaling dynamically regulates adult β cell proliferation and maturity. J Clin Invest. 2019;129:268-280 pubmed publisher
  • flow cytometry; human; 1:250; fig 4b
  • immunocytochemistry; human; 1:500; fig 4e
Shi Z, Lee K, Yang D, Amin S, Verma N, Li Q, et al. Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development. Cell Stem Cell. 2017;20:675-688.e6 pubmed publisher
  • immunohistochemistry; mouse; 1:25; loading ...; fig s2c
Li Y, Tzatzalos E, Kwan K, Grumet M, Cai L. Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development. Sci Rep. 2016;6:38665 pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; tbl 1
Massumi M, Pourasgari F, Nalla A, Batchuluun B, Nagy K, Neely E, et al. An Abbreviated Protocol for In Vitro Generation of Functional Human Embryonic Stem Cell-Derived Beta-Like Cells. PLoS ONE. 2016;11:e0164457 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 6
Cox A, Barrandon O, Cai E, Rios J, Chavez J, Bonnyman C, et al. Resolving Discrepant Findings on ANGPTL8 in ?-Cell Proliferation: A Collaborative Approach to Resolving the Betatrophin Controversy. PLoS ONE. 2016;11:e0159276 pubmed publisher
  • flow cytometry; human; 1:200; fig s19
Saxena P, Heng B, Bai P, Folcher M, Zulewski H, Fussenegger M. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells. Nat Commun. 2016;7:11247 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 3
Vegas A, Veiseh O, Gürtler M, Millman J, Pagliuca F, Bader A, et al. Long-term glycemic control using polymer-encapsulated human stem cell-derived beta cells in immune-competent mice. Nat Med. 2016;22:306-11 pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; 1:500; fig 3
Ravanelli A, Appel B. Motor neurons and oligodendrocytes arise from distinct cell lineages by progenitor recruitment. Genes Dev. 2015;29:2504-15 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Li W, Cavelti Weder C, Zhang Y, Zhang Y, Clement K, Donovan S, et al. Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells. Nat Biotechnol. 2014;32:1223-30 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 5
Maury Y, Côme J, Piskorowski R, Salah Mohellibi N, Chevaleyre V, Peschanski M, et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol. 2015;33:89-96 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50
Ediger B, Du A, Liu J, Hunter C, Walp E, Schug J, et al. Islet-1 Is essential for pancreatic ?-cell function. Diabetes. 2014;63:4206-17 pubmed publisher
Eufrásio A, Perrod C, Ferreira F, Duque M, Galhardo M, Bessa J. In Vivo Reporter Assays Uncover Changes in Enhancer Activity Caused by Type 2 Diabetes-Associated Single Nucleotide Polymorphisms. Diabetes. 2020;69:2794-2805 pubmed publisher
Ardestani A, Li S, Annamalai K, Lupse B, Geravandi S, Dobrowolski A, et al. Neratinib protects pancreatic beta cells in diabetes. Nat Commun. 2019;10:5015 pubmed publisher
Reynolds P, Holzmann Rasmussen C, Hansson M, Dufva M, Riehle M, Gadegaard N. Controlling fluid flow to improve cell seeding uniformity. PLoS ONE. 2018;13:e0207211 pubmed publisher
Burke S, Batdorf H, Burk D, Martin T, Mendoza T, Stadler K, et al. Pancreatic deletion of the interleukin-1 receptor disrupts whole body glucose homeostasis and promotes islet β-cell de-differentiation. Mol Metab. 2018;14:95-107 pubmed publisher
Lu T, Heyne S, Dror E, Casas E, Leonhardt L, Boenke T, et al. The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes. Cell Metab. 2018;27:1294-1308.e7 pubmed publisher
Memon B, Karam M, Al Khawaga S, Abdelalim E. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1. Stem Cell Res Ther. 2018;9:15 pubmed publisher
Cai H, Liu A. Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord. Dev Biol. 2017;432:72-85 pubmed publisher
Kenty J, Melton D. Testing pancreatic islet function at the single cell level by calcium influx with associated marker expression. PLoS ONE. 2015;10:e0122044 pubmed publisher
Liao X, Xue H, Wang Y, Nazor K, Guo S, Trivedi N, et al. Matched miRNA and mRNA signatures from an hESC-based in vitro model of pancreatic differentiation reveal novel regulatory interactions. J Cell Sci. 2013;126:3848-61 pubmed publisher
Spijker H, Ravelli R, Mommaas Kienhuis A, van Apeldoorn A, Engelse M, Zaldumbide A, et al. Conversion of mature human ?-cells into glucagon-producing ?-cells. Diabetes. 2013;62:2471-80 pubmed publisher
Prabakar K, Dominguez Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, et al. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant. 2012;21:1321-39 pubmed publisher
Kelly O, Chan M, Martinson L, Kadoya K, Ostertag T, Ross K, et al. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat Biotechnol. 2011;29:750-6 pubmed publisher
Heydeck W, Liu A. PCP effector proteins inturned and fuzzy play nonredundant roles in the patterning but not convergent extension of mammalian neural tube. Dev Dyn. 2011;240:1938-48 pubmed publisher
Carlsson G, Heller R, Serup P, Hyttel P. Immunohistochemistry of pancreatic development in cattle and pig. Anat Histol Embryol. 2010;39:107-19 pubmed publisher
Klinck R, Serup P, Madsen O, Jørgensen M. Specificity of four monoclonal anti-NKx6-1 antibodies. J Histochem Cytochem. 2008;56:415-24 pubmed publisher
Pedersen I, Klinck R, Hecksher Sorensen J, Zahn S, Madsen O, Serup P, et al. Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1. J Histochem Cytochem. 2006;54:567-74 pubmed
product information
Internal ID :
3348
Name :
F55A12
Depositor Name :
Madsen, O.D.
Depositor Institution :
Hagedorn Research Institute
Date Deposited :
3/15/06
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Homeobox protein Nkx-6.1; Nkx6.1
Antigen Species :
Rat
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Bovine,Human,Mouse,Porcine,Rat
Species Tested (legacy) :
mouse, rat, and human; other species not tested
Initial Publication Pubmed ID :
16401696
Depositor Notes (Special Instructions) :
This antibody is also available from the Beta Cell Consortium at http://www.betacell.org/ab/ab_view.php?AB_ID=2023
Collections :
Cell signaling,Human,Neurodevelopment,Transcription factors
Search Keywords :
Madsen, Ole D., Homeobox protein Nkx-6.1; Nkx6.1, Rat, MIgG1, kappa light chain, Mouse/Rat/Human/Bovine/Porcine, Nkx6-1, Nkx61; Nkx6a; Nkx6.1, AB_532379, monoclonal, astrocytes
Antigen Molecular Weight :
44-46 kDa
Gene :
Nkx6-1
Alternate Gene Name(s) :
Nkx61; Nkx6a; Nkx6.1
Uniprot ID :
O35762
Antibody Registry ID :
AB_532379
Immunogen :
GST- Nkx6.1 C-terminus: a.a. 299-365) developmental stage E9
Immunogen Sequence :
a.a. 299-365
Clonality :
Monoclonal
Myeloma Strain :
Fox-NY
Epitope Mapped :
Yes
Epitope Location or Sequence :
C-terminal a.a. 299-365, DDDYNKP
Epitope Map PubMed ID :
18212389 16401696
Recommended Applications :
FACS,Immunofluorescence,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
20028378 21761479 25402613 23813959
Immunofluorescence Pubmed IDs :
21804561 22195604 21761479 16401696 21804561 30440053
Western Blot Pubmed IDs :
16401696 18212389
FACS Pubmed IDs :
21804561
Depositor Growth Medium :
RPMI
DSHB Growth Medium :
RPMI
References (legacy) :
J. Histochem. Cytochem. 54(5), 567-574.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US