product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Homeobox protein Nkx-6.1; Nkx6.1
catalog :
F55A10
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F55A10
reactivity :
Spanish newt, human, mouse, rat, chicken, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 62
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; fig 2a
Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
  • flow cytometry; human; 1:2000; loading ...; tbl 1
McGaugh E, Nostro M. Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors. J Vis Exp. 2017;: pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 6
Fogarty L, Song B, Suppiah Y, Hasan S, Martin H, Hogan S, et al. Bcl-xL dependency coincides with the onset of neurogenesis in the developing mammalian spinal cord. Mol Cell Neurosci. 2016;77:34-46 pubmed publisher
  • immunocytochemistry; human; 1:300
Sagi I, Chia G, Golan Lev T, Peretz M, Weissbein U, Sui L, et al. Derivation and differentiation of haploid human embryonic stem cells. Nature. 2016;532:107-11 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 1
Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 4
Gazea M, Tasouri E, Tolve M, Bosch V, Kabanova A, Gojak C, et al. Primary cilia are critical for Sonic hedgehog-mediated dopaminergic neurogenesis in the embryonic midbrain. Dev Biol. 2016;409:55-71 pubmed publisher
  • flow cytometry; human; 1:2000; fig 3
Korytnikov R, Nostro M. Generation of polyhormonal and multipotent pancreatic progenitor lineages from human pluripotent stem cells. Methods. 2016;101:56-64 pubmed publisher
  • immunohistochemistry; chicken; 1:20; fig 2
Gurdziel K, Lorberbaum D, Udager A, Song J, Richards N, Parker D, et al. Identification and Validation of Novel Hedgehog-Responsive Enhancers Predicted by Computational Analysis of Ci/Gli Binding Site Density. PLoS ONE. 2015;10:e0145225 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 3
Huang L, Holtzinger A, Jagan I, BeGora M, Lohse I, Ngai N, et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Nat Med. 2015;21:1364-71 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 4a
Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T. Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015;5:448-59 pubmed publisher
  • immunohistochemistry; human; 1:20; fig 1
Hoeber J, Trolle C, König N, Du Z, Gallo A, Hermans E, et al. Human Embryonic Stem Cell-Derived Progenitors Assist Functional Sensory Axon Regeneration after Dorsal Root Avulsion Injury. Sci Rep. 2015;5:10666 pubmed publisher
  • immunohistochemistry; mouse; fig s5
Eisner A, Pazyra Murphy M, Durresi E, Zhou P, Zhao X, Chadwick E, et al. The Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis. Dev Cell. 2015;33:22-35 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig s5
Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, et al. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis. Nat Commun. 2015;6:6366 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 3
Veleri S, Manjunath S, Fariss R, May Simera H, Brooks M, Foskett T, et al. Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis. Nat Commun. 2014;5:4207 pubmed publisher
  • immunocytochemistry; mouse; 1:100
Akeju O, Davis Dusenbery B, Cassel S, Ichida J, Eggan K. Ketamine exposure in early development impairs specification of the primary germ cell layers. Neurotoxicol Teratol. 2014;43:59-68 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:20
Lee K, Seo J, Shin J, Ji E, Roh J, Kim J, et al. Positive feedback loop between Sox2 and Sox6 inhibits neuronal differentiation in the developing central nervous system. Proc Natl Acad Sci U S A. 2014;111:2794-9 pubmed publisher
  • immunohistochemistry - frozen section; chicken
Yan X, Lin J, Talabattula V, Mußmann C, Yang F, Wree A, et al. ADAM10 negatively regulates neuronal differentiation during spinal cord development. PLoS ONE. 2014;9:e84617 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Diaz de Durana Y, Lau J, Knee D, Filippi C, Londei M, McNamara P, et al. IL-2 immunotherapy reveals potential for innate beta cell regeneration in the non-obese diabetic mouse model of autoimmune diabetes. PLoS ONE. 2013;8:e78483 pubmed publisher
  • immunohistochemistry - free floating section; Spanish newt; 1:500
Joven A, Morona R, González A, Moreno N. Spatiotemporal patterns of Pax3, Pax6, and Pax7 expression in the developing brain of a urodele amphibian, Pleurodeles waltl. J Comp Neurol. 2013;521:3913-53 pubmed publisher
Reynolds P, Holzmann Rasmussen C, Hansson M, Dufva M, Riehle M, Gadegaard N. Controlling fluid flow to improve cell seeding uniformity. PLoS ONE. 2018;13:e0207211 pubmed publisher
Ida H, Akiyama T, Ishiguro K, Goparaju S, Nakatake Y, Chikazawa Nohtomi N, et al. Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors. Stem Cell Res Ther. 2018;9:277 pubmed publisher
Katoh M, Jung Y, Ugboma C, Shimbo M, Kuno A, Basha W, et al. MafB Is Critical for Glucagon Production and Secretion in Mouse Pancreatic α Cells In Vivo. Mol Cell Biol. 2018;38: pubmed publisher
Pusapati G, Kong J, Patel B, Krishnan A, Sagner A, Kinnebrew M, et al. CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog. Dev Cell. 2018;44:113-129.e8 pubmed publisher
Cogger K, Sinha A, Sarangi F, McGaugh E, Saunders D, Dorrell C, et al. Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors. Nat Commun. 2017;8:331 pubmed publisher
Luchetti G, Sircar R, Kong J, Nachtergaele S, Sagner A, Byrne E, et al. Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling. elife. 2016;5: pubmed publisher
Md Moin A, Dhawan S, Shieh C, Butler P, Cory M, Butler A. Increased Hormone-Negative Endocrine Cells in the Pancreas in Type 1 Diabetes. J Clin Endocrinol Metab. 2016;101:3487-96 pubmed publisher
Draney C, Hobson A, Grover S, Jack B, Tessem J. Cdk5r1 Overexpression Induces Primary β-Cell Proliferation. J Diabetes Res. 2016;2016:6375804 pubmed publisher
Ghaye A, Bergemann D, Tarifeño Saldivia E, Flasse L, Von Berg V, Peers B, et al. Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration. BMC Biol. 2015;13:70 pubmed publisher
Francius C, Ravassard P, Hidalgo Figueroa M, Mallet J, Clotman F, Nardelli J. Genetic dissection of Gata2 selective functions during specification of V2 interneurons in the developing spinal cord. Dev Neurobiol. 2015;75:721-37 pubmed publisher
Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, et al. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. elife. 2013;2:e00940 pubmed publisher
Achim K, Peltopuro P, Lahti L, Tsai H, Zachariah A, Astrand M, et al. The role of Tal2 and Tal1 in the differentiation of midbrain GABAergic neuron precursors. Biol Open. 2013;2:990-7 pubmed publisher
Hayes H, Moss L, Schisler J, Haldeman J, Zhang Z, Rosenberg P, et al. Pdx-1 activates islet ?- and ?-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2. Mol Cell Biol. 2013;33:4017-29 pubmed publisher
Tang M, Luo S, Tang V, Huang E. Temporal and spatial requirements of Smoothened in ventral midbrain neuronal development. Neural Dev. 2013;8:8 pubmed publisher
Kim K, Nam J, Mukouyama Y, Kawamoto S. Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development. J Cell Biol. 2013;200:443-58 pubmed publisher
Mukhopadhyay S, Wen X, Ratti N, Loktev A, Rangell L, Scales S, et al. The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling. Cell. 2013;152:210-23 pubmed publisher
Andersson E, Salto C, Villaescusa J, Cajanek L, Yang S, Bryjova L, et al. Wnt5a cooperates with canonical Wnts to generate midbrain dopaminergic neurons in vivo and in stem cells. Proc Natl Acad Sci U S A. 2013;110:E602-10 pubmed publisher
Abdelhamed Z, Wheway G, Szymanska K, Natarajan S, Toomes C, Inglehearn C, et al. Variable expressivity of ciliopathy neurological phenotypes that encompass Meckel-Gruber syndrome and Joubert syndrome is caused by complex de-regulated ciliogenesis, Shh and Wnt signalling defects. Hum Mol Genet. 2013;22:1358-72 pubmed publisher
Manfroid I, Ghaye A, Naye F, Detry N, Palm S, Pan L, et al. Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration. Dev Biol. 2012;366:268-78 pubmed publisher
Kuscha V, Frazer S, Dias T, Hibi M, Becker T, Becker C. Lesion-induced generation of interneuron cell types in specific dorsoventral domains in the spinal cord of adult zebrafish. J Comp Neurol. 2012;520:3604-16 pubmed publisher
Todd K, Lan Chow Wing N, Salin Cantegrel A, Cotter A, Zagami C, Lo R, et al. Establishment of motor neuron-V3 interneuron progenitor domain boundary in ventral spinal cord requires Groucho-mediated transcriptional corepression. PLoS ONE. 2012;7:e31176 pubmed publisher
Tuson M, He M, Anderson K. Protein kinase A acts at the basal body of the primary cilium to prevent Gli2 activation and ventralization of the mouse neural tube. Development. 2011;138:4921-30 pubmed publisher
Mamaeva D, Ripoll C, Bony C, Teigell M, Perrin F, Rothhut B, et al. Isolation of mineralizing Nestin+ Nkx6.1+ vascular muscular cells from the adult human spinal cord. BMC Neurosci. 2011;12:99 pubmed publisher
Conrad R, Jablonka S, Sczepan T, Sendtner M, Wiese S, Klausmeyer A. Lectin-based isolation and culture of mouse embryonic motoneurons. J Vis Exp. 2011;: pubmed publisher
Skaggs K, Martin D, Novitch B. Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling. Development. 2011;138:3199-211 pubmed publisher
Kluth O, Mirhashemi F, Scherneck S, Kaiser D, Kluge R, Neschen S, et al. Dissociation of lipotoxicity and glucotoxicity in a mouse model of obesity associated diabetes: role of forkhead box O1 (FOXO1) in glucose-induced beta cell failure. Diabetologia. 2011;54:605-16 pubmed publisher
Asli N, Kessel M. Spatiotemporally restricted regulation of generic motor neuron programs by miR-196-mediated repression of Hoxb8. Dev Biol. 2010;344:857-68 pubmed publisher
Peltopuro P, Kala K, Partanen J. Distinct requirements for Ascl1 in subpopulations of midbrain GABAergic neurons. Dev Biol. 2010;343:63-70 pubmed publisher
Ko H, Liu A, Eggenschwiler J. Analysis of hedgehog signaling in mouse intraflagellar transport mutants. Methods Cell Biol. 2009;93:347-69 pubmed publisher
Carlsson G, Heller R, Serup P, Hyttel P. Immunohistochemistry of pancreatic development in cattle and pig. Anat Histol Embryol. 2010;39:107-19 pubmed publisher
Reimer M, Kuscha V, Wyatt C, Sörensen I, Frank R, Knuwer M, et al. Sonic hedgehog is a polarized signal for motor neuron regeneration in adult zebrafish. J Neurosci. 2009;29:15073-82 pubmed publisher
Francius C, Clotman F. Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development. Neuroscience. 2010;165:116-29 pubmed publisher
Anderson R, Bosch J, Goll M, Hesselson D, Dong P, Shin D, et al. Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration. Dev Biol. 2009;334:213-23 pubmed publisher
Bayha E, Jørgensen M, Serup P, Grapin Botton A. Retinoic acid signaling organizes endodermal organ specification along the entire antero-posterior axis. PLoS ONE. 2009;4:e5845 pubmed publisher
Karumbayaram S, Novitch B, Patterson M, Umbach J, Richter L, Lindgren A, et al. Directed differentiation of human-induced pluripotent stem cells generates active motor neurons. Stem Cells. 2009;27:806-11 pubmed publisher
Cameron D, Pennimpede T, Petkovich M. Tulp3 is a critical repressor of mouse hedgehog signaling. Dev Dyn. 2009;238:1140-9 pubmed publisher
Kala K, Haugas M, Lilleväli K, Guimera J, Wurst W, Salminen M, et al. Gata2 is a tissue-specific post-mitotic selector gene for midbrain GABAergic neurons. Development. 2009;136:253-62 pubmed publisher
Dromard C, Guillon H, Rigau V, Ripoll C, Sabourin J, Perrin F, et al. Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro. J Neurosci Res. 2008;86:1916-26 pubmed publisher
Klinck R, Serup P, Madsen O, Jørgensen M. Specificity of four monoclonal anti-NKx6-1 antibodies. J Histochem Cytochem. 2008;56:415-24 pubmed publisher
Ahnfelt Rønne J, Jørgensen M, Hald J, Madsen O, Serup P, Hecksher Sørensen J. An improved method for three-dimensional reconstruction of protein expression patterns in intact mouse and chicken embryos and organs. J Histochem Cytochem. 2007;55:925-30 pubmed
Pedersen I, Klinck R, Hecksher Sorensen J, Zahn S, Madsen O, Serup P, et al. Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1. J Histochem Cytochem. 2006;54:567-74 pubmed
Karasev V. [The possibility of subcutaneous rupture of the Achilles tendon under the effect of a vertical tensile force (experimental study)]. Ortop Travmatol Protez. 1969;30:41-3 pubmed
Rook G. Mycobacteria, cytokines and antibiotics. Pathol Biol (Paris). 1990;38:276-80 pubmed
product information
Internal ID :
3343
Name :
F55A10
Depositor Name :
Madsen, O.D.
Depositor Institution :
Hagedorn Research Institute
Date Deposited :
3/15/06
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Homeobox protein Nkx-6.1; Nkx6.1
Antigen Species :
Rat
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Amphibian,Chicken,Fish,Human,Mouse,Porcine,Rat,Zebrafish
Species Tested (legacy) :
mouse, rat, chicken, pig, human, and zebrafish; other species not tested
Initial Publication Pubmed ID :
16401696
Depositor Notes (Special Instructions) :
This antibody does not cross react with Nkx6.2. F55A10 competes for binding with F55A12, but can bind simultaneously with F64A6B4 or F65A2.
Collections :
Cell signaling,Human,Neurodevelopment,Transcription factors
Search Keywords :
Madsen, Ole D., Homeobox protein Nkx-6.1; Nkx6.1, Rat, MIgG1, kappa light chain, Mouse/Rat/Chicken/Porcin/Human/Zebrafish/Amphibian, Nkx6-1, Nkx61; Nkx6a; Nkx6.1, AB_532378, monoclonal, astrocytes
Antigen Molecular Weight :
44-46 kDa
Gene :
Nkx6-1
Alternate Gene Name(s) :
Nkx61; Nkx6a; Nkx6.1
Uniprot ID :
O35762
Antibody Registry ID :
AB_532378
Immunogen :
GST- Nkx6.1 C-terminus: a.a. 299-365) developmental stage E9
Immunogen Sequence :
a.a. 299-365
Clonality :
Monoclonal
Myeloma Strain :
Fox-NY
Epitope Mapped :
Yes
Epitope Location or Sequence :
C-terminal a.a. 299-365, DDDYNKP
Epitope Map PubMed ID :
18212389 16401696
Recommended Applications :
FACS,FFPE,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
24205242 23324743 22537488 20417196 19955358 19334287 16401696 19350680 17478445 22473852 22007132 21750031 23784810 23283079 20553899 19088086 20409825 23283079 21750031 20028378 31379140
Immunofluorescence Pubmed IDs :
19955358 19800948 21946816 23332756 19350680 17478445 24501124 22363571 21946816 19631206 24205242 23938296 22473852 23420872 18335522 29249360 31412250
Western Blot Pubmed IDs :
21985235 23784810 23938296 16401696 18212389
FFPE Pubmed IDs :
24205242 2198523 20417196 31379140
FACS Pubmed IDs :
31374199
Pubmed IDs :
18335522 20409825 25369423 24501124 18335522 5369423 19334287
Depositor Growth Medium :
RPMI
DSHB Growth Medium :
RPMI
References (legacy) :
J. Histochem. Cytochem. 54(5), 567-574.; Dev. Dyn. 238, 1140-1149.; Dev. Biol. 343, 63-70.; Dev. Biol. 366, 268-278.; Cell 152, 210-223.; PLoS One 8(10):e78483. doi: 10.1371/journal.pone.0078483.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US