Notch, extracellular domain, EGF repeats #5-7 | Developmental Studies Hybridoma Bank F461.3B product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Notch, extracellular domain, EGF repeats #5-7

catalog :

F461.3B

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F461.3B

reactivity :

fruit fly

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Reference
Angulo Rojo C, Manning Cela R, Aguirre A, Ortega A, Lopez Bayghen E. Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA. ASN Neuro. 2013;5:e00130 pubmed publisher
Harris K, Tepass U. Cdc42 and Par proteins stabilize dynamic adherens junctions in the Drosophila neuroectoderm through regulation of apical endocytosis. J Cell Biol. 2008;183:1129-43 pubmed publisher
Juhasz G, Hill J, Yan Y, Sass M, Baehrecke E, Backer J, et al. The class III PI(3)K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila. J Cell Biol. 2008;181:655-66 pubmed publisher
Song X, Call G, Kirilly D, Xie T. Notch signaling controls germline stem cell niche formation in the Drosophila ovary. Development. 2007;134:1071-80 pubmed
Bland C, Rand M. Methylmercury induces activation of Notch signaling. Neurotoxicology. 2006;27:982-91 pubmed
Rawlins E, Lovegrove B, Jarman A. Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta. Development. 2003;130:6475-84 pubmed
Lawrence N, Klein T, Brennan K, Martinez Arias A. Structural requirements for notch signalling with delta and serrate during the development and patterning of the wing disc of Drosophila. Development. 2000;127:3185-95 pubmed
Rand M, Grimm L, Artavanis Tsakonas S, Patriub V, Blacklow S, Sklar J, et al. Calcium depletion dissociates and activates heterodimeric notch receptors. Mol Cell Biol. 2000;20:1825-35 pubmed

product information

Internal ID :

433

Name :

F461.3B

Depositor Name :

Artavanis-Tsakonas, S.

Depositor Institution :

Harvard Medical School

Date Deposited :

5/2/00

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Notch, extracellular domain, EGF repeats #5-7

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

10669757

Depositor Notes (Special Instructions) :

This antibody was generated against the extracellular domain of Notch containing the EGF repeats # 5-7

Collections :

Cardiac development,Cell adhesion,Cell markers,Cell signaling,Drosophila antigens,Neurodevelopment,Skeletal muscle,Transcription factors

Search Keywords :

Artavanis-Tsakonas, S., Notch, extracellular domain, EGF repeats #5-7, Drosophila, MIgG1, Drosophila, N, CG3936, Notch, NECD, NICD, AB_528409, Monoclonal, Drosophila antigens/Cell markers/Transcription factors/Neurodevelopment/Cell signaling/Cardiac development/Cell adhesion/Skeletal muscle, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 289 kDa; Apparent: 300 kDa and lower MW N-terminal fragment, resulting from proteolysis.

Gene :

Alternate Gene Name(s) :

CG3936, Notch, NECD, NICD

Uniprot ID :

P07207

Antibody Registry ID :

AB_528409

Immunogen :

Recombinant fusion protein containing the EGF repeats #5-7

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Ag8

Epitope Mapped :

Yes

Epitope Location or Sequence :

extracellular domain, EGF repeats #5-7 [see depositor notes]

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Good

Immunofluorescence Pubmed IDs :

17287246 10862754 14627723 19064670 18474623

Western Blot Pubmed IDs :

10669757 16757030 24286475

Additional Information :

The Neurogenic locus Notch protein functions as a transmembrane receptor for membrane-bound ligands Delta and Serrate to regulate neurodevelopment. Upon ligand activation, the intracellular domain (NICD) is released from the cell membrane, and forms a transcriptional activator complex with the Suppressor of hairless protein.

DSHB Growth Medium :

RPMI

References (legacy) :

Mol. Cell Biol. 20(5), 1825-1835.

more info or order :

Developmental Studies Hybridoma Bank product webpage