neurogenin 3 | Developmental Studies Hybridoma Bank F25A1B3 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

neurogenin 3

catalog :

F25A1B3

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F25A1B3

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig s1b	Gehart H, van Es J, Hamer K, Beumer J, Kretzschmar K, Dekkers J, et al. Identification of Enteroendocrine Regulators by Real-Time Single-Cell Differentiation Mapping. Cell. 2019;176:1158-1173.e16 pubmed publisher
western blot; mouse; loading ...; fig 3a	Krentz N, Van Hoof D, Li Z, Watanabe A, Tang M, Nian C, et al. Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors. Dev Cell. 2017;41:129-142.e6 pubmed publisher
flow cytometry; human; 1:1000; fig 2c	Jiang F, Li K, Archer M, Mehta M, Jamieson E, Charles A, et al. Differentiation of Islet Progenitors Regulated by Nicotinamide into Transcriptome-Verified Î² Cells That Ameliorate Diabetes. Stem Cells. 2017;35:1341-1354 pubmed publisher
immunohistochemistry - frozen section; human; 1:10; fig 2	Shamblott M, O Driscoll M, Gomez D, McGuire D. Neurogenin 3 is regulated by neurotrophic tyrosine kinase receptor type 2 (TRKB) signaling in the adult human exocrine pancreas. Cell Commun Signal. 2016;14:23 pubmed
immunocytochemistry; human; 1:100; fig 3	Saxena P, Heng B, Bai P, Folcher M, Zulewski H, Fussenegger M. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells. Nat Commun. 2016;7:11247 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 2d	Galloway J, Bethea M, Liu Y, Underwood R, Mobley J, Hunter C. SSBP3 Interacts With Islet-1 and Ldb1 to Impact Pancreatic Î²-Cell Target Genes. Mol Endocrinol. 2015;29:1774-86 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000	Smid J, Faulkes S, Rudnicki M. Periostin induces pancreatic regeneration. Endocrinology. 2015;156:824-36 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:200	Carrasco M, Delgado I, Soria B, Martin F, Rojas A. GATA4 and GATA6 control mouse pancreas organogenesis. J Clin Invest. 2012;122:3504-15 pubmed publisher
	Gupta M, De Jesus D, Kahraman S, Valdez I, Shamsi F, Yi L, et al. Insulin receptor-mediated signaling regulates pluripotency markers and lineage differentiation. Mol Metab. 2018;18:153-163 pubmed publisher
	Azizoglu D, Braitsch C, MARCIANO D, Cleaver O. Afadin and RhoA control pancreatic endocrine mass via lumen morphogenesis. Genes Dev. 2017;31:2376-2390 pubmed publisher
	Gomez D, O Driscoll M, Sheets T, Hruban R, Oberholzer J, McGarrigle J, et al. Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. PLoS ONE. 2015;10:e0133862 pubmed publisher
	Tsai Y, VanDussen K, Sawey E, Wade A, Kasper C, Rakshit S, et al. ADAM10 regulates Notch function in intestinal stem cells of mice. Gastroenterology. 2014;147:822-834.e13 pubmed publisher
	Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, et al. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. elife. 2013;2:e00940 pubmed publisher
	Ejarque M, Cervantes S, Pujadas G, Tutusaus A, Sanchez L, Gasa R. Neurogenin3 cooperates with Foxa2 to autoactivate its own expression. J Biol Chem. 2013;288:11705-17 pubmed publisher
	Simon Areces J, Acaz Fonseca E, Ruiz Palmero I, Garcia Segura L, Arevalo M. A CRM1-mediated nuclear export signal is essential for cytoplasmic localization of neurogenin 3 in neurons. PLoS ONE. 2013;8:e55237 pubmed publisher
	Tao J, Zhu M, Wang H, Afelik S, Vasievich M, Chen X, et al. SEC23B is required for the maintenance of murine professional secretory tissues. Proc Natl Acad Sci U S A. 2012;109:E2001-9 pubmed publisher
	Talchai C, Xuan S, Kitamura T, Depinho R, Accili D. Generation of functional insulin-producing cells in the gut by Foxo1 ablation. Nat Genet. 2012;44:406-12, S1 pubmed publisher
	Stamataki D, Holder M, Hodgetts C, Jeffery R, Nye E, Spencer Dene B, et al. Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system. PLoS ONE. 2011;6:e24484 pubmed publisher
	Zhang T, Saunee N, Breslin M, Song K, Lan M. Functional role of an islet transcription factor, INSM1/IA-1, on pancreatic acinar cell trans-differentiation. J Cell Physiol. 2012;227:2470-9 pubmed publisher
	Giry Laterriere M, Cherpin O, Kim Y, Jensen J, Salmon P. Polyswitch lentivectors: "all-in-one" lentiviral vectors for drug-inducible gene expression, live selection, and recombination cloning. Hum Gene Ther. 2011;22:1255-67 pubmed publisher
	Kim S, Rane S. The Cdk4-E2f1 pathway regulates early pancreas development by targeting Pdx1+ progenitors and Ngn3+ endocrine precursors. Development. 2011;138:1903-12 pubmed publisher
	Gerbe F, van Es J, Makrini L, Brulin B, Mellitzer G, Robine S, et al. Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium. J Cell Biol. 2011;192:767-80 pubmed publisher
	Cai T, Chen X, Wang R, Xu H, You Y, Zhang T, et al. Expression of insulinoma-associated 2 (INSM2) in pancreatic islet cells is regulated by the transcription factors Ngn3 and NeuroD1. Endocrinology. 2011;152:1961-9 pubmed publisher
	Ruiz Palmero I, Simon Areces J, Garcia Segura L, Arevalo M. Notch/neurogenin 3 signalling is involved in the neuritogenic actions of oestradiol in developing hippocampal neurones. J Neuroendocrinol. 2011;23:355-64 pubmed publisher
	VanDussen K, Samuelson L. Mouse atonal homolog 1 directs intestinal progenitors to secretory cell rather than absorptive cell fate. Dev Biol. 2010;346:215-23 pubmed publisher
	Mwangi S, Usta Y, Raja S, Anitha M, Chandrasekharan B, Parsadanian A, et al. Glial cell line-derived neurotrophic factor enhances neurogenin3 gene expression and beta-cell proliferation in the developing mouse pancreas. Am J Physiol Gastrointest Liver Physiol. 2010;299:G283-92 pubmed publisher
	Simon Areces J, Membrive G, Garcia Fernandez C, Garcia Segura L, Arevalo M. Neurogenin 3 cellular and subcellular localization in the developing and adult hippocampus. J Comp Neurol. 2010;518:1814-24 pubmed publisher
	Kodama M, Tsukamoto K, Yoshida K, Aoki K, Kanegasaki S, Quinn G. Embryonic stem cell transplantation correlates with endogenous neurogenin 3 expression and pancreas regeneration in streptozotocin-injured mice. J Histochem Cytochem. 2009;57:1149-58 pubmed publisher
	Du A, Hunter C, Murray J, Noble D, Cai C, Evans S, et al. Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas. Diabetes. 2009;58:2059-69 pubmed publisher
	Golson M, Le Lay J, Gao N, Brämswig N, Loomes K, Oakey R, et al. Jagged1 is a competitive inhibitor of Notch signaling in the embryonic pancreas. Mech Dev. 2009;126:687-99 pubmed publisher
	Gao N, LeLay J, Vatamaniuk M, Rieck S, Friedman J, Kaestner K. Dynamic regulation of Pdx1 enhancers by Foxa1 and Foxa2 is essential for pancreas development. Genes Dev. 2008;22:3435-48 pubmed publisher
	Fontanière S, Duvillie B, Scharfmann R, Carreira C, Wang Z, Zhang C. Tumour suppressor menin is essential for development of the pancreatic endocrine cells. J Endocrinol. 2008;199:287-98 pubmed publisher
	Yoshida T, Shiraki N, Baba H, Goto M, Fujiwara S, Kume K, et al. Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas. Genes Cells. 2008;13:667-78 pubmed publisher
	Sarkar S, Kobberup S, Wong R, Lopez A, Quayum N, Still T, et al. Global gene expression profiling and histochemical analysis of the developing human fetal pancreas. Diabetologia. 2008;51:285-97 pubmed
	Serafimidis I, Rakatzi I, Episkopou V, Gouti M, Gavalas A. Novel effectors of directed and Ngn3-mediated differentiation of mouse embryonic stem cells into endocrine pancreas progenitors. Stem Cells. 2008;26:3-16 pubmed
	Wang S, Zhang J, Zhao A, Hipkens S, Magnuson M, Gu G. Loss of Myt1 function partially compromises endocrine islet cell differentiation and pancreatic physiological function in the mouse. Mech Dev. 2007;124:898-910 pubmed
	López Díaz L, Jain R, Keeley T, VanDussen K, Brunkan C, Gumucio D, et al. Intestinal Neurogenin 3 directs differentiation of a bipotential secretory progenitor to endocrine cell rather than goblet cell fate. Dev Biol. 2007;309:298-305 pubmed
	Breslin M, Wang H, Pierce A, Aucoin R, Lan M. Neurogenin 3 recruits CBP co-activator to facilitate histone H3/H4 acetylation in the target gene INSM1. FEBS Lett. 2007;581:949-54 pubmed
	Zahn S, Hecksher Sørensen J, Pedersen I, Serup P, Madsen O. Generation of monoclonal antibodies against mouse neurogenin 3: a new immunocytochemical tool to study the pancreatic endocrine progenitor cell. Hybrid Hybridomics. 2004;23:385-8 pubmed

product information

Internal ID :

3338

Name :

F25A1B3

Depositor Name :

Madsen, O.D.

Depositor Institution :

Hagedorn Research Institute, Denmark

Date Deposited :

6/22/05

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

neurogenin 3

Antigen Species :

Mouse

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Human,Mouse

Species Tested (legacy) :

mouse, human

Initial Publication Pubmed ID :

15684667

Depositor Notes (Special Instructions) :

Fusion: 11/6/2003. Basic helix-loop-helix transcription factor required for pancreatic endocrine development. Expressed transiently during mouse pancreas development.

Collections :

Human,Neurodevelopment,Transcription factors

Search Keywords :

Madsen, Ole D., neurogenin 3, Mouse, MIgG1, kappa light chain, Mouse/Human, Neurog3, ngn3; Atoh5; Math4B; bHLHa7; Ath4b, AB_528401 AB_2251193, monoclonal, Human/Neurodevelopment/Transcription factors, Immunofluorescence/Western Blot/Chromatin Immunoprecipitation/FFPE/Gel Supershift

Antigen Molecular Weight :

Predicted: 23kDa; Apparent: 30-35kDa

Gene :

Neurog3

Alternate Gene Name(s) :

ngn3; Atoh5; Math4B; bHLHa7; Ath4b

Uniprot ID :

P70661

Antibody Registry ID :

AB_528401

Immunogen :

Recombinant partial Ngn3; GST-Ngn3 fusion protein

Immunogen Sequence :

aa 1-95

Clonality :

Monoclonal

Myeloma Strain :

Fox-NY

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 1-95

Epitope Map PubMed ID :

15684667

Recommended Applications :

Chromatin Immunoprecipitation,FFPE,Gel Supershift,Immunofluorescence,Western Blot

Immunofluorescence Pubmed IDs :

15684667 20235092 18094957 20691176 21490060 18772165 21251092 21830214 19729673 21383077 18498355 17928203 23383123 19141476 19501159 21915337 23006330 25038433 22406641 24252877 17932425 23471965 19502415 21343251 20448145 22745161 31412250

Western Blot Pubmed IDs :

21761975 23383123

Chromatin Immunoprecipitation Pubmed IDs :

21343251 17300785

FFPE Pubmed IDs :

18094957 20691176 21490060 18772165 19729673 21383077

Gel Supershift Pubmed IDs :

17300785

DSHB Growth Medium :

Iscove's

References (legacy) :

Hybrid. Hybridomics 23(6), 385-388.; Dev. Biol. 309, 298-305.; Diabetologia 51, 285-297.; J. Comp. Neurol. 518, 1814-1824.; J. Cell Biol. 192(5), 767-780.;J. Cell. Physiol. 227, 2470-2479.; PNAS 109(29), E2001-E2009.

more info or order :

Developmental Studies Hybridoma Bank product webpage