pancreatic duodenal homeobox protein 1 (Pdx1) | Developmental Studies Hybridoma Bank F109-D12 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

pancreatic duodenal homeobox protein 1 (Pdx1)

catalog :

F109-D12

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F109-D12

reactivity :

mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, FACS, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2c	Francis R, Guo H, Streutker C, Ahmed M, Yung T, Dirks P, et al. Gastrointestinal transcription factors drive lineage-specific developmental programs in organ specification and cancer. Sci Adv. 2019;5:eaax8898 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; fig 2a	Krentz N, Van Hoof D, Li Z, Watanabe A, Tang M, Nian C, et al. Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors. Dev Cell. 2017;41:129-142.e6 pubmed publisher
	Campbell S, McDonald C, Krentz N, Lynn F, Hoffman B. TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation. Cell Rep. 2019;28:1830-1844.e6 pubmed publisher
	Bianchi M, Bianchi S, Hernado InsÃºa A, Martinez L, Lago N, Libertun C, et al. Proposed mechanisms for oligonucleotide IMT504 induced diabetes reversion in a mouse model of immunodependent diabetes. Am J Physiol Endocrinol Metab. 2016;311:E380-95 pubmed publisher
	Petzold K, Spagnoli F. A system for ex vivo culturing of embryonic pancreas. J Vis Exp. 2012;:e3979 pubmed publisher
	Galbo T, Pedersen I, Fløyel T, Bang Berthelsen C, Serup P, Madsen O, et al. Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels. Eur J Histochem. 2010;54:e19 pubmed

product information

Internal ID :

3333

Name :

F109-D12

Depositor Name :

Madsen, O.D.

Depositor Institution :

Hagedorn Research Institute, Denmark

Date Deposited :

2/28/07

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

pancreatic duodenal homeobox protein 1 (Pdx1)

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Mouse,Rat

Species Tested (legacy) :

mouse and rat Pdx1

Initial Publication Pubmed ID :

20558340

Depositor Notes (Special Instructions) :

Pdx1 is a homeobox transcription factor required for pancreas formation. F6A11 doesn't recognize human Pdx1.

Collections :

Cell signaling,Nucleus,Transcription factors

Search Keywords :

Madsen, O.D., pancreatic duodenal homeobox protein 1 (Pdx1) , Rat, MIgG1, kappa light chain, Mouse/Rat, Pdx1, Ipf1, Idx1, Stf1, AB_1157903, monoclonal, Cell signaling/Transcription factors/Nucleus, Immunohistochemistry/ELISA/Immunofluorescence/Western Blot/FFPE/FACS

Antigen Molecular Weight :

Predicted: 31kDa; Apparent: 39 kDa and additional band at 70 kDa

Gene :

Pdx1

Alternate Gene Name(s) :

Ipf1, Idx1, Stf1

Uniprot ID :

P52947

Antibody Registry ID :

AB_1157903

Synonyms (Alternate Clone Names) :

F109

Immunogen :

Recombinant GST/Pdx1 (aa 215-283) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Fox-NY

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal aa 215-283.

Epitope Map PubMed ID :

20558340

Recommended Applications :

ELISA,FACS,FFPE,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

27329801

Immunofluorescence Pubmed IDs :

20558340 31412250

Western Blot Pubmed IDs :

20558340

ELISA Pubmed IDs :

20558340

FFPE Pubmed IDs :

20558340

FACS Pubmed IDs :

20558340

Pubmed IDs :

22951988

DSHB Growth Medium :

RPMI

References (legacy) :

Eur. J. Histochem. 54(2), 92-98.

more info or order :

Developmental Studies Hybridoma Bank product webpage