ERM-1 | Developmental Studies Hybridoma Bank ERM1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

ERM-1

catalog :

ERM1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ERM1

reactivity :

Caenorhabditis elegans

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 9

Reference
Von Stetina S, Liang J, Marnellos G, Mango S. Temporal regulation of epithelium formation mediated by FoxA, MKLP1, MgcRacGAP, and PAR-6. Mol Biol Cell. 2017;28:2042-2065 pubmed publisher
Geisler F, Gerhardus H, Carberry K, Davis W, Jorgensen E, Richardson C, et al. A novel function for the MAP kinase SMA-5 in intestinal tube stability. Mol Biol Cell. 2016;27:3855-3868 pubmed
Budirahardja Y, Doan T, Zaidel Bar R. Glycosyl phosphatidylinositol anchor biosynthesis is essential for maintaining epithelial integrity during Caenorhabditis elegans embryogenesis. PLoS Genet. 2015;11:e1005082 pubmed publisher
Saegusa K, Sato M, Sato K, Nakajima Shimada J, Harada A, Sato K. Caenorhabditis elegans chaperonin CCT/TRiC is required for actin and tubulin biogenesis and microvillus formation in intestinal epithelial cells. Mol Biol Cell. 2014;25:3095-104 pubmed publisher
Grün D, Kirchner M, Thierfelder N, Stoeckius M, Selbach M, Rajewsky N. Conservation of mRNA and protein expression during development of C. elegans. Cell Rep. 2014;6:565-77 pubmed publisher
Patel F, Soto M. WAVE/SCAR promotes endocytosis and early endosome morphology in polarized C. elegans epithelia. Dev Biol. 2013;377:319-32 pubmed publisher
Cheng L, Li B, Wang Z. Level-Set Minimization of Potential Controlled Hadwiger Valuations for Molecular Solvation. J Comput Phys. 2010;229:8497-8510 pubmed
Bernadskaya Y, Patel F, Hsu H, Soto M. Arp2/3 promotes junction formation and maintenance in the Caenorhabditis elegans intestine by regulating membrane association of apical proteins. Mol Biol Cell. 2011;22:2886-99 pubmed publisher
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher

product information

Internal ID :

6478

Name :

ERM1

Depositor Name :

Nonet, M.L. / Hadwiger, G. / Dour, S.

Depositor Institution :

Washington University Medical School

Date Deposited :

2/16/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

ERM-1

Antigen Species :

C. elegans

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

C. elegans

Species Tested (legacy) :

C. elegans

Initial Publication Pubmed ID :

20405020

Depositor Notes (Special Instructions) :

Works well on C. elegans whole mounts. Stains apical membranes.

Collections :

C. elegans

Search Keywords :

Nonet, M.L. / Hadwiger, G. / Dour, S., ERM-1, C. elegans, MIgG1, C. elegans, erm-1, , AB_10584795, monoclonal, C. elegans, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 66 kDa; Apparent: 72 kDa

Gene :

erm-1

Uniprot ID :

G5EES2

Antibody Registry ID :

AB_10584795

Synonyms (Alternate Clone Names) :

5G2

Immunogen :

Recombinant His6-tagged HMR-1 (C-terminal aa 209-563) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal aa 209-563

Epitope Map PubMed ID :

20405020

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

good

Immunohistochemistry Pubmed IDs :

25807459

Immunofluorescence Pubmed IDs :

20405020 21697505 25143409 23510716 31390561

Western Blot Pubmed IDs :

20405020 24462290 21697505 27733627

ELISA Pubmed IDs :

20405020

Additional Information :

ERM-1 is a homolog of the ezrin-radixin-moesin (ERM) family of cytoskeletal linker proteins. It localizes most prominently to the apical membranes in the polarized alimentary tract, but is also found on the apical cell cortex of many cells during development.

DSHB Growth Medium :

Iscove's

References (legacy) :

Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. (2010) A monoclonal antibody toolkit for C. elegans. PLoS One. 5(4):e10161-10115.

more info or order :

Developmental Studies Hybridoma Bank product webpage