primordial germ cell surface marker (mouse) | Developmental Studies Hybridoma Bank EMA-1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

primordial germ cell surface marker (mouse)

catalog :

EMA-1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

EMA-1

application :

western blot, immunohistochemistry, immunocytochemistry, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Mak S, Alev C, Nagai H, Wrabel A, Matsuoka Y, Honda A, et al. Characterization of the finch embryo supports evolutionary conservation of the naive stage of development in amniotes. elife. 2015;4:e07178 pubmed publisher
Raucci F, Fuet A, Pain B. In vitro generation and characterization of chicken long-term germ cells from different embryonic origins. Theriogenology. 2015;84:732-42.e1-2 pubmed publisher
Zhang S, Sun J, Pan S, Zhu H, Wang L, Hu Y, et al. Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis. J Cell Biochem. 2011;112:1009-21 pubmed publisher
Hua J, Yu H, Dong W, Yang C, Gao Z, Lei A, et al. Characterization of mesenchymal stem cells (MSCs) from human fetal lung: potential differentiation of germ cells. Tissue Cell. 2009;41:448-55 pubmed publisher
Chen X, Li L, Wong C, Cheng S. Rapid adaptation of molecular resources from zebrafish and medaka to develop an estuarine/marine model. Comp Biochem Physiol C Toxicol Pharmacol. 2009;149:647-55 pubmed publisher
Mozdziak P, Angerman Stewart J, Rushton B, Pardue S, Petitte J. Isolation of chicken primordial germ cells using fluorescence-activated cell sorting. Poult Sci. 2005;84:594-600 pubmed
Yamazaki Y, Mann M, Lee S, Marh J, McCarrey J, Yanagimachi R, et al. Reprogramming of primordial germ cells begins before migration into the genital ridge, making these cells inadequate donors for reproductive cloning. Proc Natl Acad Sci U S A. 2003;100:12207-12 pubmed
Turnpenny L, Brickwood S, Spalluto C, Piper K, Cameron I, Wilson D, et al. Derivation of human embryonic germ cells: an alternative source of pluripotent stem cells. Stem Cells. 2003;21:598-609 pubmed
Pain B, Clark M, Shen M, Nakazawa H, Sakurai M, Samarut J, et al. Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities. Development. 1996;122:2339-48 pubmed
Hahnel A, Eddy E. The distribution of two cell surface determinants of mouse embryonal carcinoma and early embryonic cells. J Reprod Immunol. 1987;10:89-110 pubmed
McCarrey J, Hsu K, Eddy E, Klevecz R, Bolen J. Isolation of viable mouse primordial germ cells by antibody-directed flow sorting. J Exp Zool. 1987;242:107-11 pubmed

product information

Internal ID :

2470

Name :

EMA-1

Depositor Name :

Eddy, M. / Hahnel, A.

Depositor Institution :

NIH, NIEHS, RTP and Ontario Veterinary College

Date Deposited :

9/7/94

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

primordial germ cell surface marker (mouse)

Antigen Species :

Mouse

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Amphibian,Avian,Broad species,Fish,Mammal,Zebrafish

Species Tested (legacy) :

multiple species (mammal, birds, fish, amphibia)

Initial Publication Pubmed ID :

3598980

Depositor Notes (Special Instructions) :

This antibody binds specifically to the surface of primordial germ cells and a variety of adult apical epithelium including oviduct and proximal kidney. This antibody also recognizes antigen in olfactory pits and membranous portions of fins in zebrafish PMID: 19302835.

Collections :

Cell markers,Human,Stem cell

Search Keywords :

Eddy, M. / Hahnel, A., primordial germ cell surface marker (mouse), Mouse, MIgM, broad species/mammal/avian/fish/Amphibian, , , AB_531885, monoclonal, Cell markers/Human/Stem cell, Immunohistochemistry/Immunofluorescence/Western Blot/FFPE/FACS, EMA1

Antigen Molecular Weight :

Apparent: +500 kDa

Antibody Registry ID :

AB_531885

Immunogen :

Nulli SCC1 EC cells (fixed in 1% formaldehyde + 1% glutaraldehyde)

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

EMA-1 recognizes short chain (monofucosylated polylactosamine) and long chain (mono- and difucosylated polylactosamine) glycolipids of cell surface glycoproteins specific of primordial germ cells.

Epitope Map PubMed ID :

3598980

Recommended Applications :

FACS,FFPE,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

3598980 12968114 19651422 15844816

Immunofluorescence Pubmed IDs :

14506296 3598509 26037665 19302835 21308744 8756279

Western Blot Pubmed IDs :

3598980

FFPE Pubmed IDs :

3598980 14506296

FACS Pubmed IDs :

3598509

Additional Information :

This antibody was originally described and characterized in [Hahnel, A.C., and Eddy, E.M. (1986). Cell surface markers of mouse primordial germ cells defined by two monoclonal antibodies. Gamete Research 15, 25-34]. RRID:AB_531885

DSHB Growth Medium :

RPMI

References (legacy) :

Gamete Res. 15, 25-34.; J. Repro. Immun. 10, 89-110.; PNAS 100, 12207-12212.; Stem Cells 21, 598-609.

more info or order :

Developmental Studies Hybridoma Bank product webpage