Pericardin | Developmental Studies Hybridoma Bank EC11 anti-Pericardin product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Pericardin

catalog :

EC11 anti-Pericardin

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

EC11 anti-Pericardin

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 22

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:30; loading ...; fig 3E	Raza Q, Vanderploeg J, Jacobs J. Matrix Metalloproteinases are required for membrane motility and lumenogenesis during Drosophila heart development. PLoS ONE. 2017;12:e0171905 pubmed publisher
	Hollfelder D, Frasch M, Reim I. Distinct functions of the laminin β LN domain and collagen IV during cardiac extracellular matrix formation and stabilization of alary muscle attachments revealed by EMS mutagenesis in Drosophila. BMC Dev Biol. 2014;14:26 pubmed publisher
	Horn L, Leips J, Starz Gaiano M. Phagocytic ability declines with age in adult Drosophila hemocytes. Aging Cell. 2014;13:719-28 pubmed publisher
	Mirzoyan Z, Pandur P. The Iroquois complex is required in the dorsal mesoderm to ensure normal heart development in Drosophila. PLoS ONE. 2013;8:e76498 pubmed publisher
	Drechsler M, Schmidt A, Meyer H, Paululat A. The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity. PLoS Genet. 2013;9:e1003616 pubmed publisher
	Tögel M, Meyer H, Lehmacher C, Heinisch J, Pass G, Paululat A. The bHLH transcription factor hand is required for proper wing heart formation in Drosophila. Dev Biol. 2013;381:446-59 pubmed publisher
	Macabenta F, Jensen A, Cheng Y, Kramer J, Kramer S. Frazzled/DCC facilitates cardiac cell outgrowth and attachment during Drosophila dorsal vessel formation. Dev Biol. 2013;380:233-42 pubmed publisher
	Na J, Musselman L, Pendse J, Baranski T, Bodmer R, Ocorr K, et al. A Drosophila model of high sugar diet-induced cardiomyopathy. PLoS Genet. 2013;9:e1003175 pubmed publisher
	Tauc H, Mann T, Werner K, Pandur P. A role for Drosophila Wnt-4 in heart development. Genesis. 2012;50:466-81 pubmed publisher
	Wang S, Meyer H, Ochoa Espinosa A, Buchwald U, Onel S, Altenhein B, et al. GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis. J Cell Sci. 2012;125:461-72 pubmed publisher
	Yang S, Su M. Excessive Dpp signaling induces cardial apoptosis through dTAK1 and dJNK during late embryogenesis of Drosophila. J Biomed Sci. 2011;18:85 pubmed publisher
	Wesley C, Guo H, Chaudhry K, THALI M, Yin J, Clason T, et al. Loss of PTB or negative regulation of Notch mRNA reveals distinct zones of Notch and actin protein accumulation in Drosophila embryo. PLoS ONE. 2011;6:e21876 pubmed publisher
	Meyer H, Panz M, Albrecht S, Drechsler M, Wang S, Hüsken M, et al. Drosophila metalloproteases in development and differentiation: the role of ADAM proteins and their relatives. Eur J Cell Biol. 2011;90:770-8 pubmed publisher
	Wolfstetter G, Holz A. The role of LamininB2 (LanB2) during mesoderm differentiation in Drosophila. Cell Mol Life Sci. 2012;69:267-82 pubmed publisher
	Mann T, Bodmer R, Pandur P. The Drosophila homolog of vertebrate Islet1 is a key component in early cardiogenesis. Development. 2009;136:317-26 pubmed publisher
	Llorens J, Navarro J, Martínez Sebastián M, Baylies M, Schneuwly S, Botella J, et al. Causative role of oxidative stress in a Drosophila model of Friedreich ataxia. FASEB J. 2007;21:333-44 pubmed
	SANTIAGO MARTINEZ E, Soplop N, Kramer S. Lateral positioning at the dorsal midline: Slit and Roundabout receptors guide Drosophila heart cell migration. Proc Natl Acad Sci U S A. 2006;103:12441-6 pubmed
	Perrin L, Monier B, Ponzielli R, Astier M, Semeriva M. Drosophila cardiac tube organogenesis requires multiple phases of Hox activity. Dev Biol. 2004;272:419-31 pubmed
	Ponzielli R, Astier M, Chartier A, Gallet A, Therond P, Semeriva M. Heart tube patterning in Drosophila requires integration of axial and segmental information provided by the Bithorax Complex genes and hedgehog signaling. Development. 2002;129:4509-21 pubmed
	Chartier A, Zaffran S, Astier M, Semeriva M, Gratecos D. Pericardin, a Drosophila type IV collagen-like protein is involved in the morphogenesis and maintenance of the heart epithelium during dorsal ectoderm closure. Development. 2002;129:3241-53 pubmed
	Frémion F, Astier M, Zaffran S, Guillen A, Homburger V, Semeriva M. The heterotrimeric protein Go is required for the formation of heart epithelium in Drosophila. J Cell Biol. 1999;145:1063-76 pubmed
	Zaffran S, Astier M, Gratecos D, Guillen A, Semeriva M. Cellular interactions during heart morphogenesis in the Drosophila embryo. Biol Cell. 1995;84:13-24 pubmed

product information

Internal ID :

3682

Name :

EC11 anti-Pericardin

Depositor Name :

Gratecos, Danielle

Depositor Institution :

Laboratoire de Genetique et Physiologie du Developpement (LGPD) / CNRS

Date Deposited :

2/19/02

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Pericardin

Antigen Species :

Drosophila melanogaster

Host Species :

mouse

Isotype :

MIgG3

Isotype for catalog (legacy) :

IgG3

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8574195

Depositor Notes (Special Instructions) :

Fusion: 1986. Immunohistology: On whole mount Drosophila embryos, it decorates the extracellular matrix surrounding the pericardial cells and the cardial cells of the heart tube, from 10h embryogenesis onwards; it is synthesized and secreted by the pericardial cells; it also labels the ring gland and the oenocytes; no maternal expression; it is still present around the adult heart tube. Immunoblotting with EC11: three bands (200, 190, 165 kDa) differentially expressed during embryogenesis, the 165 kDa species being the only one to persist in the adult heart tube. Functional effects: In the absence of Pericardin, the heart epithelium appears disorganized during its migration in concert with the dorsal ectoderm. The alignment of the cardial and pericardial cells is interrupted by holes and both types of cells are found accumulated in clusters along the rows of cardioblasts. The protein is probably involved in the morphogenesis and in the maintenance of the heart epithelium during dorsal ectoderm closure. Immunohistochemistry: Pericardial cells, extracellular matrix surrounding the heart tube, ring gland, oenocytes.

Collections :

Cardiac development,Drosophila antigens,Extracellular matrix proteins

Search Keywords :

Gratecos, Danielle, Pericardin, Drosophila melanogaster, MIgG3, Drosophila, prc, pericardin; pc, AB_528431, monoclonal, Cardiac development/Drosophila antigens/Extracellular matrix proteins, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 165kDa; Apparent: 200, 190 and 165kDa

Gene :

prc

Alternate Gene Name(s) :

pericardin; pc

Uniprot ID :

Q9U617

Antibody Registry ID :

AB_528431

Immunogen :

Nitrocellulose containing embryonic (10-14 hours Drosophila melanogaster, Oregon R embryos) proteins after western blot. The excised nitrocellulose band contained proteins migrating at the same level than myosin heavy chain.

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63-Ag.8-653

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8574195 21750738 10352022 22114909 17167074

Immunofluorescence Pubmed IDs :

8574195 21684629 23326243 16888037 12070098 19088091 22302994 23685255 21387145 24828474 12223408 22371299 15282158 23874219 23747982 24086746 24935095 10352022 29599288

Western Blot Pubmed IDs :

8574195 23326243 12070098 23874219 29599288

Immunoprecipitation Pubmed IDs :

12070098

Depositor Growth Medium :

Iscove's

Additional Information :

Pericardin is a type IV collagen-like ECM protein contributing to the ECM layer that surrounds the myocardial–pericardial network. EC1 1 was isolated by chance in a screen for monoclonal antibodies recognizing the muscle myosin heavy chain [PMID 3117889]. There are three Uniprot entries for this protein, two (Q7YU48 and Q9U617) are supported by experimental evidence at transcript level; the third, Q9VTR6, is a predicted protein; all three proteins are quite different; Q9U617 was selected as Uniprot ID because it encodes the NFQSTYYTK peptide identified in [PMID 12070098] by immunoprecipitation and sequencing. In [PMID 12070098], EC11 was used for immunogold on EM.

DSHB Growth Medium :

Iscove's

References (legacy) :

Biol. Cell 84, 13-24.; Development, submitted.

more info or order :

Developmental Studies Hybridoma Bank product webpage