product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Transitin
catalog :
EAP3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
EAP3
reactivity :
chicken
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 17
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; chicken; 1:50; fig 2
Peretz Y, Eren N, Kohl A, Hen G, Yaniv K, Weisinger K, et al. A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2. BMC Biol. 2016;14:57 pubmed publisher
  • immunohistochemistry; chicken; 1:50; fig 8
Gallina D, Zelinka C, Cebulla C, Fischer A. Activation of glucocorticoid receptors in Müller glia is protective to retinal neurons and suppresses microglial reactivity. Exp Neurol. 2015;273:114-25 pubmed publisher
  • immunohistochemistry; chicken; 1:50
Fischer A, Zelinka C, Milani Nejad N. Reactive retinal microglia, neuronal survival, and the formation of retinal folds and detachments. Glia. 2015;63:313-27 pubmed publisher
  • immunohistochemistry; chicken; 1:80
Fischer A, Zelinka C, Gallina D, Scott M, Todd L. Reactive microglia and macrophage facilitate the formation of Müller glia-derived retinal progenitors. Glia. 2014;62:1608-28 pubmed publisher
Matsushita T, Steinfeld J, Fujihara A, Urayama S, Taketani S, Araki M. Regulation of neuronal and photoreceptor cell differentiation by Wnt signaling from iris-derived stem/progenitor cells of the chick in flat vs. Matrigel-embedding cultures. Brain Res. 2019;1704:207-218 pubmed publisher
Todd L, Squires N, Suarez L, Fischer A. Jak/Stat signaling regulates the proliferation and neurogenic potential of Müller glia-derived progenitor cells in the avian retina. Sci Rep. 2016;6:35703 pubmed publisher
Silva T, França G, Ornelas I, Loiola E, Ulrich H, Ventura A. Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures. Purinergic Signal. 2015;11:183-201 pubmed publisher
Ornelas I, Silva T, Fragel Madeira L, Ventura A. Inhibition of PI3K/Akt pathway impairs G2/M transition of cell cycle in late developing progenitors of the avian embryo retina. PLoS ONE. 2013;8:e53517 pubmed publisher
Zelinka C, Scott M, Volkov L, Fischer A. The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1. PLoS ONE. 2012;7:e44477 pubmed publisher
Cebulla C, Zelinka C, Scott M, Lubow M, Bingham A, Rasiah S, et al. A chick model of retinal detachment: cone rich and novel. PLoS ONE. 2012;7:e44257 pubmed publisher
Bello S, Millo H, Rajebhosale M, Price S. Catenin-dependent cadherin function drives divisional segregation of spinal motor neurons. J Neurosci. 2012;32:490-505 pubmed publisher
Ghai K, Zelinka C, Fischer A. Notch signaling influences neuroprotective and proliferative properties of mature Müller glia. J Neurosci. 2010;30:3101-12 pubmed publisher
King L, Schwartz N, Domowicz M. Glial migratory streams in the developing hindbrain: a slice culture approach. J Neurosci Methods. 2009;177:30-43 pubmed publisher
Wakamatsu Y, Nakamura N, Lee J, Cole G, Osumi N. Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells. Development. 2007;134:2425-33 pubmed
Henion P, Blyss G, Luo R, An M, Maynard T, Cole G, et al. Avian transitin expression mirrors glial cell fate restrictions during neural crest development. Dev Dyn. 2000;218:150-9 pubmed
Yuan Y, Lee J, Napier A, Cole G. Molecular cloning of a new intermediate filament protein expressed by radial glia and demonstration of alternative splicing in a novel heptad repeat region located in the carboxy-terminal tail domain. Mol Cell Neurosci. 1997;10:71-86 pubmed
Cole G, Lee J. Immunocytochemical localization of a novel radial glial intermediate filament protein. Brain Res Dev Brain Res. 1997;101:225-38 pubmed
product information
Internal ID :
1781
Name :
EAP3
Depositor Name :
Cole, G.J.
Depositor Institution :
North Carolina Central University, Durham
Depositor Website :
http://www.nccu.edu/directory/details.cfm?id=gcole
Date Deposited :
9/25/03
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Transitin
Antigen Species :
Chick
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Chick
Species Tested (legacy) :
chicken
Initial Publication Pubmed ID :
9361289
Depositor Notes (Special Instructions) :
EAP3 labels the intermediate filament network in radial glia and myoblasts. This antigen is also known as EAP-300.
Collections :
Cytoskeleton,Neurodevelopment
Search Keywords :
Cole, G.J., Transitin, Chick, MIgG1, kappa light chain, Chick, , , AB_2282449, Monoclonal, Neurodevelopment, Immunofluorescence/Immunohistochemistry
Antigen Molecular Weight :
300 kDa apparent/ 202 kDa derived
Uniprot ID :
Q90831
Antibody Registry ID :
AB_2282449
Immunogen :
Purified transitin from E9 brain
Immunogen Sequence :
Full length sequence
Clonality :
Monoclonal
Myeloma Strain :
Sp2/0
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
9263595 18948137 22238085 22970190 27392568 27759082
Immunofluorescence Pubmed IDs :
9361289 9263595 17522158 30347217 20181607 22973454 23301080 25231952 25663277 26272753
Western Blot Pubmed IDs :
10822267
Additional Information :
RRID:AB_2282449
DSHB Growth Medium :
Iscove's
References (legacy) :
Dev. Brain Res. 66, 11-23.; Mol. Cell. Neurosci. 10, 71-86.; Dev. Brain Res. 101, 225-238.; Dev. Brain Res. 109, 59-66.; J. Mol. Neurosci. 12, 11-22.; J. Comp. Neurol. 418, 473-483.; Dev. Dyn. 218, 150-159.; Histochem. Cell Biol. 116, 397-409.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US