product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Transitin
catalog :
EAP3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
EAP3
reactivity :
chicken
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
more info or order :
citations: 17
Published Application/Species/Sample/Dilution | Reference |
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Wakamatsu Y, Nakamura N, Lee J, Cole G, Osumi N. Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells. Development. 2007;134:2425-33 pubmed
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Henion P, Blyss G, Luo R, An M, Maynard T, Cole G, et al. Avian transitin expression mirrors glial cell fate restrictions during neural crest development. Dev Dyn. 2000;218:150-9 pubmed
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Yuan Y, Lee J, Napier A, Cole G. Molecular cloning of a new intermediate filament protein expressed by radial glia and demonstration of alternative splicing in a novel heptad repeat region located in the carboxy-terminal tail domain. Mol Cell Neurosci. 1997;10:71-86 pubmed
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Cole G, Lee J. Immunocytochemical localization of a novel radial glial intermediate filament protein. Brain Res Dev Brain Res. 1997;101:225-38 pubmed
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product information
Internal ID :
1781
Name :
EAP3
Depositor Name :
Cole, G.J.
Depositor Institution :
North Carolina Central University, Durham
Depositor Website :
http://www.nccu.edu/directory/details.cfm?id=gcole
Date Deposited :
9/25/03
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Transitin
Antigen Species :
Chick
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Chick
Species Tested (legacy) :
chicken
Initial Publication Pubmed ID :
9361289
Depositor Notes (Special Instructions) :
EAP3 labels the intermediate filament network in radial glia and myoblasts. This antigen is also known as EAP-300.
Collections :
Cytoskeleton,Neurodevelopment
Search Keywords :
Cole, G.J., Transitin, Chick, MIgG1, kappa light chain, Chick, , , AB_2282449, Monoclonal, Neurodevelopment, Immunofluorescence/Immunohistochemistry
Antigen Molecular Weight :
300 kDa apparent/ 202 kDa derived
Uniprot ID :
Q90831
Antibody Registry ID :
AB_2282449
Immunogen :
Purified transitin from E9 brain
Immunogen Sequence :
Full length sequence
Clonality :
Monoclonal
Myeloma Strain :
Sp2/0
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
9263595 18948137 22238085 22970190 27392568 27759082
Immunofluorescence Pubmed IDs :
9361289 9263595 17522158 30347217 20181607 22973454 23301080 25231952 25663277 26272753
Western Blot Pubmed IDs :
10822267
Additional Information :
RRID:AB_2282449
DSHB Growth Medium :
Iscove's
References (legacy) :
Dev. Brain Res. 66, 11-23.; Mol. Cell. Neurosci. 10, 71-86.; Dev. Brain Res. 101, 225-238.; Dev. Brain Res. 109, 59-66.; J. Mol. Neurosci. 12, 11-22.; J. Comp. Neurol. 418, 473-483.; Dev. Dyn. 218, 150-159.; Histochem. Cell Biol. 116, 397-409.
more info or order :
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Developmental Studies Hybridoma Bank
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