Patched (extracellular region) | Developmental Studies Hybridoma Bank Drosophila Ptc (Apa 1) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Patched (extracellular region)

catalog :

Drosophila Ptc (Apa 1)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Drosophila Ptc (Apa 1)

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:250; loading ...; fig 7a	Ducuing A, Vincent S. The actin cable is dispensable in directing dorsal closure dynamics but neutralizes mechanical stress to prevent scarring in the Drosophila embryo. Nat Cell Biol. 2016;18:1149-1160 pubmed publisher
immunohistochemistry; fruit fly; 1:50; fig 5	Gurdziel K, Lorberbaum D, Udager A, Song J, Richards N, Parker D, et al. Identification and Validation of Novel Hedgehog-Responsive Enhancers Predicted by Computational Analysis of Ci/Gli Binding Site Density. PLoS ONE. 2015;10:e0145225 pubmed publisher
	Bras Pereira C, Potier D, Jacobs J, Aerts S, Casares F, Janody F. dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. PLoS Genet. 2016;12:e1006204 pubmed publisher
	Jiang K, Jia J. Analysis of Smoothened Phosphorylation and Activation in Cultured Cells and Wing Discs of Drosophila. Methods Mol Biol. 2015;1322:45-60 pubmed publisher
	Mao F, Yang X, Fu L, Lv X, Zhang Z, Wu W, et al. The Kto-Skd complex can regulate ptc expression by interacting with Cubitus interruptus (Ci) in the Hedgehog signaling pathway. J Biol Chem. 2014;289:22333-41 pubmed publisher
	Wang G, Tang X, Chen Y, Cao J, Huang Q, Ling X, et al. Hyperplastic discs differentially regulates the transcriptional outputs of hedgehog signaling. Mech Dev. 2014;133:117-25 pubmed publisher
	Huang S, Zhang Z, Zhang C, Lv X, Zheng X, Chen Z, et al. Activation of Smurf E3 ligase promoted by smoothened regulates hedgehog signaling through targeting patched turnover. PLoS Biol. 2013;11:e1001721 pubmed publisher
	Shi D, Lv X, Zhang Z, Yang X, Zhou Z, Zhang L, et al. Smoothened oligomerization/higher order clustering in lipid rafts is essential for high Hedgehog activity transduction. J Biol Chem. 2013;288:12605-14 pubmed publisher
	Christiansen A, Ding T, Fan Y, Graves H, Herz H, Lindblad J, et al. Non-cell autonomous control of apoptosis by ligand-independent Hedgehog signaling in Drosophila. Cell Death Differ. 2013;20:302-11 pubmed publisher
	Fan J, Liu Y, Jia J. Hh-induced Smoothened conformational switch is mediated by differential phosphorylation at its C-terminal tail in a dose- and position-dependent manner. Dev Biol. 2012;366:172-84 pubmed publisher
	Hartman T, Zinshteyn D, Schofield H, Nicolas E, Okada A, O Reilly A. Drosophila Boi limits Hedgehog levels to suppress follicle stem cell proliferation. J Cell Biol. 2010;191:943-52 pubmed publisher
	Raisin S, Ruel L, Ranieri N, Staccini Lavenant L, Thérond P. Dynamic phosphorylation of the kinesin Costal-2 in vivo reveals requirement of fused kinase activity for all levels of hedgehog signalling. Dev Biol. 2010;344:119-28 pubmed publisher
	Hödl M, Basler K. Transcription in the absence of histone H3.3. Curr Biol. 2009;19:1221-6 pubmed publisher
	Callejo A, Quijada L, Guerrero I. Detecting tagged Hedgehog with intracellular and extracellular immunocytochemistry for functional analysis. Methods Mol Biol. 2007;397:91-103 pubmed
	Gorfinkiel N, Sierra J, Callejo A, Ibañez C, Guerrero I. The Drosophila ortholog of the human Wnt inhibitor factor Shifted controls the diffusion of lipid-modified Hedgehog. Dev Cell. 2005;8:241-53 pubmed
	Torroja C, Gorfinkiel N, Guerrero I. Patched controls the Hedgehog gradient by endocytosis in a dynamin-dependent manner, but this internalization does not play a major role in signal transduction. Development. 2004;131:2395-408 pubmed
	Martin V, Carrillo G, Torroja C, Guerrero I. The sterol-sensing domain of Patched protein seems to control Smoothened activity through Patched vesicular trafficking. Curr Biol. 2001;11:601-7 pubmed
	Capdevila J, Estrada M, Sánchez Herrero E, Guerrero I. The Drosophila segment polarity gene patched interacts with decapentaplegic in wing development. EMBO J. 1994;13:71-82 pubmed
	Capdevila J, Pariente F, Sampedro J, Alonso J, Guerrero I. Subcellular localization of the segment polarity protein patched suggests an interaction with the wingless reception complex in Drosophila embryos. Development. 1994;120:987-98 pubmed

product information

Internal ID :

3748

Name :

Drosophila Ptc (Apa 1)

Depositor Name :

Guerrero, I.

Depositor Institution :

Universidad Autonoma de Madrid

Date Deposited :

10/7/05

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Patched (extracellular region)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a, kappa light chain

Positive Tested Species Reactivity :

Drosophila,Lizard

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7600973

Depositor Notes (Special Instructions) :

Drosophila Hedgehog receptor, ptc mutant embryos show a segment polarity phenotype

Collections :

Cell markers,Cell signaling,Drosophila antigens

Search Keywords :

Guerrero, I., Patched (extracellular region), Drosophila, MIgG2a, kappa light chain, Drosophila, ptc, CG2411, ptch, AB_528441, monoclonal, Drosophila antigens/Cell signaling/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 143 kDa

Gene :

ptc

Alternate Gene Name(s) :

CG2411, ptch

Uniprot ID :

P18502

Antibody Registry ID :

AB_528441

Synonyms (Alternate Clone Names) :

Apa 1.3

Immunogen :

Recombinant fusion protein containing the first extracellular loop of Patched, aa 106-346.

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

NP-3

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 106-346

Epitope Map PubMed ID :

7600973

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8306973 7600973 30086335

Immunofluorescence Pubmed IDs :

8306973 7600973 11369205 15102702 15691765 22537496 24854243 24302888 18025716 23018595 24962581 23532857 19523831 20435030 21098113 26179038 27442438

Western Blot Pubmed IDs :

24302888

Additional Information :

For experiments involving live cells and tissue, Drosophila Ptc (Apa 1) supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.

DSHB Growth Medium :

Iscove's

References (legacy) :

EMBO J. 13, 71-82.; Development 120, 987-998.; Curr. Biol. 11, 1-7.; Development 131(10), 2395-2408.; Dev. Cell 8, 1-13.; Dev. Biol. 366, 172-184.

more info or order :

Developmental Studies Hybridoma Bank product webpage