Green fluorescent protein (GFP) | Developmental Studies Hybridoma Bank DSHB-GFP-4C9 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Green fluorescent protein (GFP)

catalog :

DSHB-GFP-4C9

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DSHB-GFP-4C9

application :

ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Reference
Ashley J, Cordy B, Lucia D, Fradkin L, Budnik V, Thomson T. Retrovirus-like Gag Protein Arc1 Binds RNA and Traffics across Synaptic Boutons. Cell. 2018;172:262-274.e11 pubmed publisher
Krey J, Dumont R, Wilmarth P, David L, Johnson K, Barr Gillespie P. ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells. J Neurosci. 2018;38:843-857 pubmed publisher
Tsukamoto T, Gearhart M, Spike C, Huelgas Morales G, Mews M, Boag P, et al. LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans. Genetics. 2017;206:2007-2039 pubmed publisher
Le Thomas A, Stuwe E, Li S, Du J, Marinov G, Rozhkov N, et al. Transgenerationally inherited piRNAs trigger piRNA biogenesis by changing the chromatin of piRNA clusters and inducing precursor processing. Genes Dev. 2014;28:1667-80 pubmed publisher
Sanchez P, Daniels K, Park Y, Soll D. Generating a battery of monoclonal antibodies against native green fluorescent protein for immunostaining, FACS, IP, and ChIP using a unique adjuvant. Monoclon Antib Immunodiagn Immunother. 2014;33:80-8 pubmed publisher

product information

Internal ID :

8436

Name :

DSHB-GFP-4C9

Depositor Name :

DSHB

Depositor Institution :

University of Iowa

Depositor Website :

http://dshb.biology.uiowa.edu/

Date Deposited :

4/15/13

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Green fluorescent protein (GFP)

Antigen Species :

Aequorea victoria

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Aequorea victoria,All species for fusion proteins

Initial Publication Pubmed ID :

24746148

Depositor Notes (Special Instructions) :

DSHB--GFP-4C9 does not western blot. mAb 4C9 cross-reacts with YFP.

Collections :

GFP,Protein tags

Search Keywords :

Developmental Studies Hybridoma Bank, Green fluorescent protein (GFP), Aequorea victoria, MIgG1, kappa light chain, Aequorea victoria/All species for fusion proteins, GFP, , , Monoclonal

Antigen Molecular Weight :

26.8 kDa

Gene :

GFP

Uniprot ID :

P42224

Antibody Registry ID :

AB_2617422

Immunogen :

Recombinant protein

Immunogen Sequence :

Full length

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0-Ag14

Epitope Mapped :

Recommended Applications :

Chromatin Immunoprecipitation,ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

Immunofluorescence Pubmed IDs :

24746148 28576864 29328915 27161498 28617241

Immunoprecipitation Pubmed IDs :

24746148

Chromatin Immunoprecipitation Pubmed IDs :

25085419 24746148

FFPE Pubmed IDs :

24746148

FACS Pubmed IDs :

24746148

Pubmed IDs :

24746148 25085419

Depositor Growth Medium :

RPMI-1640 plus 15% FCS + 20% medium conditioned by giant cell tumor TIB-223 (ATCC)

Additional Information :

4C9 also recognizes eGFP, BFP, CFP and YFP but not mCherry or RFP as determined by ELISA. RRID:AB_2617422

DSHB Growth Medium :

Iscove's

References (legacy) :

Monoclon. Antib. Immunodiagn. Immunother. 33(2), 80-88.; Genes Dev. 28, 1667-1680.

more info or order :

Developmental Studies Hybridoma Bank product webpage