Green fluorescent protein (GFP) | Developmental Studies Hybridoma Bank DSHB-GFP-12E6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Green fluorescent protein (GFP)

catalog :

DSHB-GFP-12E6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DSHB-GFP-12E6

application :

ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 3

Reference
Garelli A, Heredia F, Casimiro A, Macedo A, Nunes C, Garcez M, et al. Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing. Nat Commun. 2015;6:8732 pubmed publisher
Wessels D, Lusche D, Scherer A, Kuhl S, Myre M, Soll D. Huntingtin regulates Ca(2+) chemotaxis and K(+)-facilitated cAMP chemotaxis, in conjunction with the monovalent cation/H(+) exchanger Nhe1, in a model developmental system: insights into its possible role in Huntington×³s disease. Dev Biol. 2014;394:24-38 pubmed publisher
Sanchez P, Daniels K, Park Y, Soll D. Generating a battery of monoclonal antibodies against native green fluorescent protein for immunostaining, FACS, IP, and ChIP using a unique adjuvant. Monoclon Antib Immunodiagn Immunother. 2014;33:80-8 pubmed publisher

product information

Internal ID :

8424

Name :

DSHB-GFP-12E6

Depositor Name :

DSHB

Depositor Institution :

University of Iowa

Depositor Website :

http://dshb.biology.uiowa.edu/

Date Deposited :

4/15/13

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

Green fluorescent protein (GFP)

Antigen Species :

Aequorea victoria

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Aequorea victoria,All species for fusion proteins

Initial Publication Pubmed ID :

24746148

Depositor Notes (Special Instructions) :

DSHB-GFP-12E6 does not western blot.

Collections :

GFP,Protein tags

Search Keywords :

Developmental Studies Hybridoma Bank, Green fluorescent protein (GFP), Aequorea victoria, MIgG1, kappa light chain, Aequorea victoria/All species for fusion proteins, GFP, , , Monoclonal

Antigen Molecular Weight :

26.8 kDa

Gene :

GFP

Uniprot ID :

P42212

Antibody Registry ID :

AB_2617418

Immunogen :

Recombinant protein

Immunogen Sequence :

Full length

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0-Ag14

Epitope Mapped :

Recommended Applications :

Chromatin Immunoprecipitation,ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

yes, non-reduced (native) dot blot

Immunofluorescence Pubmed IDs :

24746148 25149514

Immunoprecipitation Pubmed IDs :

24746148

Chromatin Immunoprecipitation Pubmed IDs :

24746148

FFPE Pubmed IDs :

24746148

FACS Pubmed IDs :

24746148

Pubmed IDs :

24746148

Depositor Growth Medium :

RPMI-1640 plus 15% FCS + 20% medium conditioned by giant cell tumor TIB-223 (ATCC)

Additional Information :

12E6 also recognizes eGFP and BFP but not mCherry or RFP as determined by ELISA. RRID:AB_2617418

DSHB Growth Medium :

Iscove's

References (legacy) :

Monoclon. Antib. Immunodiagn. Immunother. 33(2), 80-88.

more info or order :

Developmental Studies Hybridoma Bank product webpage