product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Green fluorescent protein (GFP)
catalog :
DSHB-GFP-12A6
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DSHB-GFP-12A6
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
more info or order :
citations: 14
Reference |
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Tsukamoto T, Gearhart M, Spike C, Huelgas Morales G, Mews M, Boag P, et al. LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans. Genetics. 2017;206:2007-2039 pubmed publisher
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product information
Internal ID :
8439
Name :
DSHB-GFP-12A6
Depositor Name :
DSHB
Depositor Institution :
University of Iowa
Depositor Website :
http://dshb.biology.uiowa.edu/
Date Deposited :
4/15/13
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Green fluorescent protein (GFP)
Antigen Species :
Aequorea victoria
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Aequorea victoria,All species for fusion proteins
Initial Publication Pubmed ID :
24746148
Depositor Notes (Special Instructions) :
DSHB-GFP-12A6 does not western blot.
Collections :
GFP,Protein tags
Search Keywords :
Developmental Studies Hybridoma Bank, Green fluorescent protein (GFP), Aequorea victoria, MIgG1, kappa light chain, Aequorea victoria/All species for fusion proteins, GFP, , , Monoclonal
Antigen Molecular Weight :
26.8 kDa
Gene :
GFP
Uniprot ID :
P42212
Antibody Registry ID :
AB_2617417
Immunogen :
Recombinant protein
Immunogen Sequence :
Full length
Clonality :
Monoclonal
Myeloma Strain :
Sp2/0-Ag14
Epitope Mapped :
No
Recommended Applications :
Chromatin Immunoprecipitation,ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation
Immunoblotting (legacy) :
yes, non-reduced (native) dot blot
Immunofluorescence Pubmed IDs :
24746148 26518186 27693235 28576864 29162808 29753016 31329635
Immunoprecipitation Pubmed IDs :
24746148
Chromatin Immunoprecipitation Pubmed IDs :
24746148 26814702 26814702
FFPE Pubmed IDs :
24746148
FACS Pubmed IDs :
24746148
Pubmed IDs :
24746148
Depositor Growth Medium :
RPMI-1640 plus 15% FCS + 20% medium conditioned by giant cell tumor TIB-223 (ATCC)
Additional Information :
12A6 also recognizes eGFP and BFP but not mCherry or RFP as determined by ELISA. RRID:AB_2617417
DSHB Growth Medium :
Iscove's
References (legacy) :
Monoclon. Antib. Immunodiagn. Immunother. 33(2), 80-88.
more info or order :
company information
Developmental Studies Hybridoma Bank