product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
cadherin, DN- (extracellular domain)
catalog :
DN-Ex #8
clonality :
monoclonal
host :
brown rat
conjugate :
nonconjugated
clone name :
DN-Ex #8
reactivity :
Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
Developmental Studies Hybridoma Bank product webpage
citations: 27
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Drosophila melanogaster; 1:500; loading ...; fig 1a
Zhang X, Coates K, Dacks A, G nay C, Lauritzen J, Li F, et al. Local synaptic inputs support opposing, network-specific odor representations in a widely projecting modulatory neuron. elife. 2019;8: pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:40; loading ...; fig 3a
Guajardo R, Luginbuhl D, Han S, Luo L, Li J. Functional divergence of Plexin B structural motifs in distinct steps of Drosophila olfactory circuit assembly. elife. 2019;8: pubmed publisher
Letizia A, He D, Astigarraga S, Colombelli J, Hatini V, Llimargas M, et al. Sidekick Is a Key Component of Tricellular Adherens Junctions that Acts to Resolve Cell Rearrangements. Dev Cell. 2019;50:313-326.e5 pubmed publisher
Agrawal P, Chung P, Heberlein U, Kent C. Enabling cell-type-specific behavioral epigenetics in Drosophila: a modified high-yield INTACT method reveals the impact of social environment on the epigenetic landscape in dopaminergic neurons. BMC Biol. 2019;17:30 pubmed publisher
White K, Grillo Hill B, Esquivel M, Peralta J, Bui V, Chire I, et al. β-Catenin is a pH sensor with decreased stability at higher intracellular pH. J Cell Biol. 2018;217:3965-3976 pubmed publisher
Ratheesh A, Biebl J, Veselá J, Smutny M, Papusheva E, Krens S, et al. Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration. Dev Cell. 2018;45:331-346.e7 pubmed publisher
Hinaux H, Bachem K, Battistara M, Rossi M, Xin Y, Jaenichen R, et al. Revisiting the developmental and cellular role of the pigmentation gene yellow in Drosophila using a tagged allele. Dev Biol. 2018;438:111-123 pubmed publisher
Peng J, Santiago I, Ahn C, Gur B, Tsui C, Su Z, et al. Drosophila Fezf coordinates laminar-specific connectivity through cell-intrinsic and cell-extrinsic mechanisms. elife. 2018;7: pubmed publisher
Morrison C, Chen H, Cook T, Brown S, Treisman J. Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye. PLoS Genet. 2018;14:e1007173 pubmed publisher
Kendroud S, Bohra A, Kuert P, Nguyen B, Guillermin O, Sprecher S, et al. Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments. J Comp Neurol. 2018;526:33-58 pubmed publisher
Shao L, Saver M, Chung P, Ren Q, Lee T, Kent C, et al. Dissection of the Drosophila neuropeptide F circuit using a high-throughput two-choice assay. Proc Natl Acad Sci U S A. 2017;114:E8091-E8099 pubmed publisher
Anzo M, Sekine S, Makihara S, Chao K, Miura M, Chihara T. Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in Drosophila. Genes Dev. 2017;31:1054-1065 pubmed publisher
Kidd S, Lieber T. Mechanism of Notch Pathway Activation and Its Role in the Regulation of Olfactory Plasticity in Drosophila melanogaster. PLoS ONE. 2016;11:e0151279 pubmed publisher
Shepherd D, Harris R, Williams D, Truman J. Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres. J Comp Neurol. 2016;524:2677-95 pubmed publisher
Omoto J, Yogi P, Hartenstein V. Origin and development of neuropil glia of the Drosophila larval and adult brain: Two distinct glial populations derived from separate progenitors. Dev Biol. 2015;404:2-20 pubmed publisher
Lovick J, Ngo K, Omoto J, Wong D, Nguyen J, Hartenstein V. Postembryonic lineages of the Drosophila brain: I. Development of the lineage-associated fiber tracts. Dev Biol. 2013;384:228-57 pubmed publisher
Mellert D, ROBINETT C, Baker B. doublesex functions early and late in gustatory sense organ development. PLoS ONE. 2012;7:e51489 pubmed publisher
Dottermusch Heidel C, Groth V, Beck L, Onel S. The Arf-GEF Schizo/Loner regulates N-cadherin to induce fusion competence of Drosophila myoblasts. Dev Biol. 2012;368:18-27 pubmed publisher
Venken K, Schulze K, Haelterman N, Pan H, He Y, Evans Holm M, et al. MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nat Methods. 2011;8:737-43 pubmed
Spindler S, Hartenstein V. Bazooka mediates secondary axon morphology in Drosophila brain lineages. Neural Dev. 2011;6:16 pubmed publisher
Potter C, Tasic B, Russler E, Liang L, Luo L. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. Cell. 2010;141:536-48 pubmed publisher
Young J, Armstrong J. Building the central complex in Drosophila: the generation and development of distinct neural subsets. J Comp Neurol. 2010;518:1525-41 pubmed publisher
Kumar A, Fung S, Lichtneckert R, Reichert H, Hartenstein V. Arborization pattern of engrailed-positive neural lineages reveal neuromere boundaries in the Drosophila brain neuropil. J Comp Neurol. 2009;517:87-104 pubmed publisher
Martinek N, Shahab J, Saathoff M, Ringuette M. Haemocyte-derived SPARC is required for collagen-IV-dependent stability of basal laminae in Drosophila embryos. J Cell Sci. 2008;121:1671-80 pubmed publisher
Iyengar B, Chou C, Sharma A, Atwood H. Modular neuropile organization in the Drosophila larval brain facilitates identification and mapping of central neurons. J Comp Neurol. 2006;499:583-602 pubmed
Iwai Y, Hirota Y, Ozaki K, Okano H, Takeichi M, Uemura T. DN-cadherin is required for spatial arrangement of nerve terminals and ultrastructural organization of synapses. Mol Cell Neurosci. 2002;19:375-88 pubmed
Iwai Y, Usui T, Hirano S, Steward R, Takeichi M, Uemura T. Axon patterning requires DN-cadherin, a novel neuronal adhesion receptor, in the Drosophila embryonic CNS. Neuron. 1997;19:77-89 pubmed
product information
Internal ID :
886
Name :
DN-Ex #8
Depositor Name :
Uemura, T.
Depositor Institution :
Kyoto Univ. Graduate Sch.of Biostudies
Date Deposited :
1/7/04
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
cadherin, DN- (extracellular domain)
Antigen Species :
Drosophila
Host Species :
rat
Isotype :
RIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
9247265
Depositor Notes (Special Instructions) :
Suitable to use in immunostaining and western blots. 1/ Staining of embryos and discs; both fixative 1 (3.7% formaldehyde in PBS) and fixative 2 (4% paraformaldehyde in CMF & GBS containing 1mM CaCl2 (see details in Dev. Biol. 165, 716-726)) work. 2/ Staining of S2 cells; fixative 1 doesn't work, use fixative 2. 3/ Staining of pupal or adult heads for light microscope; find details in our MCN paper and references therein such as Uchida et al. (4% paraformaldehyde-based fixative on ice for 1-2 hr) and Hoshino et al. (PLP-based fixative on ice for 1-2 hr). For better fixation, we cut pupal or adult heads into halves. 4? ImmunoEM of adult heads; find details in our MCN paper. We do not add EDTA or EGTA in any fixatives, because cadherins are easily degraded under Ca++- free conditions.
Collections :
Cell adhesion,Cell markers,Drosophila antigens,Neurodevelopment
Search Keywords :
Uemura, T., cadherin, DN- (extracellular domain), Drosophila, RIgG2a, Drosophila, CadN, CG7100, N-cadh, AB_528121, monoclonal, Drosophila antigens/Cell adhesion/Neurodevelopment/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot
Antigen Molecular Weight :
Predicted: 347 kDa; Apparent: 300 and 200 kDa
Gene :
CadN
Alternate Gene Name(s) :
CG7100, N-cadh
Uniprot ID :
O15943
Antibody Registry ID :
AB_528121
Immunogen :
Recombinant fusion protein containing the extracellular region of N-cadherin from cadherin repeats 2 to 8.
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Myeloma Strain :
P3-X63-Ag8-U1
Epitope Mapped :
Yes
Epitope Location or Sequence :
extracellular region including CR2-CR8
Epitope Map PubMed ID :
9247265
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
9247265 11906210 21985007 31353315
Immunofluorescence Pubmed IDs :
9247265 20434990 19711412 23880429 25779704 26986723 23240029 21246549 21985007 20187144 17029252 18445681 21524279 22595515 28874527 28875566 26878258 29324767 29634916 29738712
Western Blot Pubmed IDs :
9247265
Additional Information :
Marker of neuropil
DSHB Growth Medium :
Iscove's
References (legacy) :
Neuron 19, 77-89.; Mol. Cell. Neurosci. 19, 375-388.; Cell 141(3), 536-548.
more info or order :
Developmental Studies Hybridoma Bank product webpage
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US