product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
DLG-1 (Discs Large)
catalog :
DLG1
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DLG1
reactivity :
fruit fly, Caenorhabditis elegans
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 74
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly; 1:200; loading ...; fig 1c
Fujita N, Huang W, Lin T, Groulx J, Jean S, Nguyen J, et al. Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. elife. 2017;6: pubmed publisher
  • immunohistochemistry; fruit fly; 1:20; fig 2
Zwarts L, Goossens T, Clements J, Kang Y, Callaerts P. Axon Branch-Specific Semaphorin-1a Signaling in Drosophila Mushroom Body Development. Front Cell Neurosci. 2016;10:210 pubmed publisher
Hauswirth A, Ford K, Wang T, Fetter R, Tong A, Davis G. A postsynaptic PI3K-cII dependent signaling controller for presynaptic homeostatic plasticity. elife. 2018;7: pubmed publisher
Hart K, Tan J, Siemers K, Sim J, PRUITT B, Nelson W, et al. E-cadherin and LGN align epithelial cell divisions with tissue tension independently of cell shape. Proc Natl Acad Sci U S A. 2017;114:E5845-E5853 pubmed publisher
Tare M, Sarkar A, Bedi S, Kango Singh M, Singh A. Cullin-4 regulates Wingless and JNK signaling-mediated cell death in the Drosophila eye. Cell Death Dis. 2016;7:e2566 pubmed publisher
Chen X, Wang Y, Chen Y, Harry B, Nakagawa A, Lee E, et al. Regulation of CED-3 caspase localization and activation by C. elegans nuclear-membrane protein NPP-14. Nat Struct Mol Biol. 2016;23:958-964 pubmed publisher
Morelli E, Mastrodonato V, Beznoussenko G, Mironov A, Tognon E, Vaccari T. An essential step of kinetochore formation controlled by the SNARE protein Snap29. EMBO J. 2016;35:2223-2237 pubmed
Padash Barmchi M, GILBERT M, Thomas M, Banks L, Zhang B, Auld V. A Drosophila Model of HPV E6-Induced Malignancy Reveals Essential Roles for Magi and the Insulin Receptor. PLoS Pathog. 2016;12:e1005789 pubmed publisher
Rodrigues F, Shao W, Harris T. The Arf GAP Asap promotes Arf1 function at the Golgi for cleavage furrow biosynthesis in Drosophila. Mol Biol Cell. 2016;27:3143-3155 pubmed
Xu S, Panikker P, Iqbal S, Elefant F. Tip60 HAT Action Mediates Environmental Enrichment Induced Cognitive Restoration. PLoS ONE. 2016;11:e0159623 pubmed publisher
Meehan T, Joudi T, Timmons A, Taylor J, Habib C, Peterson J, et al. Components of the Engulfment Machinery Have Distinct Roles in Corpse Processing. PLoS ONE. 2016;11:e0158217 pubmed publisher
Hunter G, Hadjivasiliou Z, Bonin H, He L, Perrimon N, Charras G, et al. Coordinated control of Notch/Delta signalling and cell cycle progression drives lateral inhibition-mediated tissue patterning. Development. 2016;143:2305-10 pubmed publisher
Esmaeeli Nieh S, Fenckova M, Porter I, Motazacker M, Nijhof B, Castells Nobau A, et al. BOD1 Is Required for Cognitive Function in Humans and Drosophila. PLoS Genet. 2016;12:e1006022 pubmed publisher
Padash Barmchi M, Samarasekera G, GILBERT M, Auld V, Zhang B. Magi Is Associated with the Par Complex and Functions Antagonistically with Bazooka to Regulate the Apical Polarity Complex. PLoS ONE. 2016;11:e0153259 pubmed publisher
Lee D, Rodrigues F, Yu C, Swan M, Harris T. PH Domain-Arf G Protein Interactions Localize the Arf-GEF Steppke for Cleavage Furrow Regulation in Drosophila. PLoS ONE. 2015;10:e0142562 pubmed publisher
Meehan T, Kleinsorge S, Timmons A, Taylor J, McCall K. Polarization of the epithelial layer and apical localization of integrins are required for engulfment of apoptotic cells in the Drosophila ovary. Dis Model Mech. 2015;8:1603-14 pubmed publisher
Natarajan R, Barber K, Buckley A, Cho P, Egbejimi A, Wairkar Y. Tricornered Kinase Regulates Synapse Development by Regulating the Levels of Wiskott-Aldrich Syndrome Protein. PLoS ONE. 2015;10:e0138188 pubmed publisher
Hunter M, Lee D, Harris T, Fernandez Gonzalez R. Polarized E-cadherin endocytosis directs actomyosin remodeling during embryonic wound repair. J Cell Biol. 2015;210:801-16 pubmed publisher
Zhou B, Lindsay S, Wasserman S. Alternative NF-κB Isoforms in the Drosophila Neuromuscular Junction and Brain. PLoS ONE. 2015;10:e0132793 pubmed publisher
Niehues S, Bussmann J, Steffes G, Erdmann I, Köhrer C, Sun L, et al. Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. Nat Commun. 2015;6:7520 pubmed publisher
Antonello Z, Reiff T, Ballesta Illan E, Dominguez M. Robust intestinal homeostasis relies on cellular plasticity in enteroblasts mediated by miR-8-Escargot switch. EMBO J. 2015;34:2025-41 pubmed publisher
Lee D, Wilk R, Hu J, Krause H, Harris T. Germ Cell Segregation from the Drosophila Soma Is Controlled by an Inhibitory Threshold Set by the Arf-GEF Steppke. Genetics. 2015;200:863-72 pubmed publisher
Gnerer J, Venken K, Dierick H. Gene-specific cell labeling using MiMIC transposons. Nucleic Acids Res. 2015;43:e56 pubmed publisher
Liu J, Lee D, Yu C, Angers S, Harris T. Stepping stone: a cytohesin adaptor for membrane cytoskeleton restraint in the syncytial Drosophila embryo. Mol Biol Cell. 2015;26:711-25 pubmed publisher
Haralalka S, Shelton C, Cartwright H, Guo F, Trimble R, Kumar R, et al. Live imaging provides new insights on dynamic F-actin filopodia and differential endocytosis during myoblast fusion in Drosophila. PLoS ONE. 2014;9:e114126 pubmed publisher
Kato M, Chou T, Yu C, Demodena J, Sternberg P. LINKIN, a new transmembrane protein necessary for cell adhesion. elife. 2014;3:e04449 pubmed publisher
Ghosh R, Vegesna S, Safi R, Bao H, Zhang B, Marenda D, et al. Kismet positively regulates glutamate receptor localization and synaptic transmission at the Drosophila neuromuscular junction. PLoS ONE. 2014;9:e113494 pubmed publisher
Jumbo Lucioni P, Parkinson W, Broadie K. Overelaborated synaptic architecture and reduced synaptomatrix glycosylation in a Drosophila classic galactosemia disease model. Dis Model Mech. 2014;7:1365-78 pubmed publisher
Kang M, Hansen T, Mickiewicz M, Kaczynski T, Fye S, Gunawardena S. Disruption of axonal transport perturbs bone morphogenetic protein (BMP)--signaling and contributes to synaptic abnormalities in two neurodegenerative diseases. PLoS ONE. 2014;9:e104617 pubmed publisher
Morais de Sá E, Mukherjee A, Lowe N, St Johnston D. Slmb antagonises the aPKC/Par-6 complex to control oocyte and epithelial polarity. Development. 2014;141:2984-92 pubmed publisher
Huu N, Yoshida H, Umegawachi T, Miyata S, Yamaguchi M. Structural characterization and subcellular localization of Drosophila organic solute carrier partner 1. BMC Biochem. 2014;15:11 pubmed publisher
Masuda Nakagawa L, Ito K, Awasaki T, O Kane C. A single GABAergic neuron mediates feedback of odor-evoked signals in the mushroom body of larval Drosophila. Front Neural Circuits. 2014;8:35 pubmed publisher
Gamblin C, Hardy E, Chartier F, Bisson N, Laprise P. A bidirectional antagonism between aPKC and Yurt regulates epithelial cell polarity. J Cell Biol. 2014;204:487-95 pubmed publisher
Haack T, Bergstralh D, St Johnston D. Damage to the Drosophila follicle cell epithelium produces "false clones" with apparent polarity phenotypes. Biol Open. 2013;2:1313-20 pubmed publisher
Moran M, Tare M, Kango Singh M, Singh A. Homeotic Gene teashirt (tsh) has a neuroprotective function in amyloid-beta 42 mediated neurodegeneration. PLoS ONE. 2013;8:e80829 pubmed publisher
Steffensmeier A, Tare M, Puli O, Modi R, Nainaparampil J, Kango Singh M, et al. Novel neuroprotective function of apical-basal polarity gene crumbs in amyloid beta 42 (aβ42) mediated neurodegeneration. PLoS ONE. 2013;8:e78717 pubmed publisher
Oortveld M, Keerthikumar S, Oti M, Nijhof B, Fernandes A, Kochinke K, et al. Human intellectual disability genes form conserved functional modules in Drosophila. PLoS Genet. 2013;9:e1003911 pubmed publisher
Southall T, Gold K, Egger B, Davidson C, Caygill E, Marshall O, et al. Cell-type-specific profiling of gene expression and chromatin binding without cell isolation: assaying RNA Pol II occupancy in neural stem cells. Dev Cell. 2013;26:101-12 pubmed publisher
Enneking E, Kudumala S, Moreno E, Stephan R, Boerner J, Godenschwege T, et al. Transsynaptic coordination of synaptic growth, function, and stability by the L1-type CAM Neuroglian. PLoS Biol. 2013;11:e1001537 pubmed publisher
Chang L, Kreko T, Davison H, Cusmano T, Wu Y, Rothenfluh A, et al. Normal dynactin complex function during synapse growth in Drosophila requires membrane binding by Arfaptin. Mol Biol Cell. 2013;24:1749-64, S1-5 pubmed publisher
Tanji T, Nishikori K, Shiraishi H, Maeda M, Ohashi Kobayashi A. Co-operative function and mutual stabilization of the half ATP-binding cassette transporters HAF-4 and HAF-9 in Caenorhabditis elegans. Biochem J. 2013;452:467-75 pubmed publisher
Gómez J, Wang Y, Riechmann V. Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis. J Cell Biol. 2012;199:1131-43 pubmed publisher
Aranjuez G, Kudlaty E, Longworth M, McDonald J. On the role of PDZ domain-encoding genes in Drosophila border cell migration. G3 (Bethesda). 2012;2:1379-91 pubmed publisher
McKinley R, Harris T. Displacement of basolateral Bazooka/PAR-3 by regulated transport and dispersion during epithelial polarization in Drosophila. Mol Biol Cell. 2012;23:4465-71 pubmed publisher
Raghu S, Claussen J, Borst A. Neurons with GABAergic phenotype in the visual system of Drosophila. J Comp Neurol. 2013;521:252-65 pubmed publisher
Thomas A, Lee P, Dalton J, Nomie K, Stoica L, Costa Mattioli M, et al. A versatile method for cell-specific profiling of translated mRNAs in Drosophila. PLoS ONE. 2012;7:e40276 pubmed publisher
Zhang H, Kim A, Abraham N, Khan L, Hall D, Fleming J, et al. Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis. Development. 2012;139:2071-83 pubmed publisher
Mukherjee T, Choi I, Banerjee U. Genetic analysis of fibroblast growth factor signaling in the Drosophila eye. G3 (Bethesda). 2012;2:23-8 pubmed publisher
Marrone A, Kucherenko M, Wiek R, Gopfert M, Shcherbata H. Hyperthermic seizures and aberrant cellular homeostasis in Drosophila dystrophic muscles. Sci Rep. 2011;1:47 pubmed publisher
Mauser J, Prehoda K. Inscuteable regulates the Pins-Mud spindle orientation pathway. PLoS ONE. 2012;7:e29611 pubmed publisher
Wee B, Johnston C, Prehoda K, Doe C. Canoe binds RanGTP to promote Pins(TPR)/Mud-mediated spindle orientation. J Cell Biol. 2011;195:369-76 pubmed publisher
Bernadskaya Y, Patel F, Hsu H, Soto M. Arp2/3 promotes junction formation and maintenance in the Caenorhabditis elegans intestine by regulating membrane association of apical proteins. Mol Biol Cell. 2011;22:2886-99 pubmed publisher
Siller S, Broadie K. Neural circuit architecture defects in a Drosophila model of Fragile X syndrome are alleviated by minocycline treatment and genetic removal of matrix metalloproteinase. Dis Model Mech. 2011;4:673-85 pubmed publisher
Dimitriadi M, Sleigh J, Walker A, Chang H, Sen A, Kalloo G, et al. Conserved genes act as modifiers of invertebrate SMN loss of function defects. PLoS Genet. 2010;6:e1001172 pubmed publisher
Varija Raghu S, Reiff D, Borst A. Neurons with cholinergic phenotype in the visual system of Drosophila. J Comp Neurol. 2011;519:162-76 pubmed publisher
Bayraktar O, Boone J, Drummond M, Doe C. Drosophila type II neuroblast lineages keep Prospero levels low to generate large clones that contribute to the adult brain central complex. Neural Dev. 2010;5:26 pubmed publisher
Liebl F, McKeown C, Yao Y, HING H. Mutations in Wnt2 alter presynaptic motor neuron morphology and presynaptic protein localization at the Drosophila neuromuscular junction. PLoS ONE. 2010;5:e12778 pubmed publisher
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher
Campbell K, Casanova J, Skaer H. Mesenchymal-to-epithelial transition of intercalating cells in Drosophila renal tubules depends on polarity cues from epithelial neighbours. Mech Dev. 2010;127:345-57 pubmed publisher
Seabrooke S, Qiu X, Stewart B. Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophila neuromuscular junction. BMC Neurosci. 2010;11:37 pubmed publisher
Hamanaka Y, Meinertzhagen I. Immunocytochemical localization of synaptic proteins to photoreceptor synapses of Drosophila melanogaster. J Comp Neurol. 2010;518:1133-55 pubmed publisher
Roeth J, Sawyer J, Wilner D, Peifer M. Rab11 helps maintain apical crumbs and adherens junctions in the Drosophila embryonic ectoderm. PLoS ONE. 2009;4:e7634 pubmed publisher
Fuentes Medel Y, Logan M, Ashley J, Ataman B, Budnik V, Freeman M. Glia and muscle sculpt neuromuscular arbors by engulfing destabilized synaptic boutons and shed presynaptic debris. PLoS Biol. 2009;7:e1000184 pubmed publisher
Grusche F, Hidalgo C, Fletcher G, Sung H, Sahai E, Thompson B. Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]. BMC Dev Biol. 2009;9:14 pubmed publisher
Kapelnikov A, Rivlin P, Hoy R, Heifetz Y. Tissue remodeling: a mating-induced differentiation program for the Drosophila oviduct. BMC Dev Biol. 2008;8:114 pubmed publisher
Chang H, Dimlich D, Yokokura T, Mukherjee A, Kankel M, Sen A, et al. Modeling spinal muscular atrophy in Drosophila. PLoS ONE. 2008;3:e3209 pubmed publisher
Haussmann I, White K, Soller M. Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways. Genome Biol. 2008;9:R73 pubmed publisher
Ly C, Yao C, Verstreken P, Ohyama T, Bellen H. straightjacket is required for the synaptic stabilization of cacophony, a voltage-gated calcium channel alpha1 subunit. J Cell Biol. 2008;181:157-70 pubmed publisher
Besse F, Mertel S, Kittel R, Wichmann C, Rasse T, Sigrist S, et al. The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses. J Cell Biol. 2007;177:843-55 pubmed
Acharya U, Edwards M, Jorquera R, Silva H, Nagashima K, Labarca P, et al. Drosophila melanogaster Scramblases modulate synaptic transmission. J Cell Biol. 2006;173:69-82 pubmed
Harris T, Peifer M. The positioning and segregation of apical cues during epithelial polarity establishment in Drosophila. J Cell Biol. 2005;170:813-23 pubmed
Sherwood N, Sun Q, Xue M, Zhang B, Zinn K. Drosophila spastin regulates synaptic microtubule networks and is required for normal motor function. PLoS Biol. 2004;2:e429 pubmed
Harris T, Peifer M. Adherens junction-dependent and -independent steps in the establishment of epithelial cell polarity in Drosophila. J Cell Biol. 2004;167:135-47 pubmed
Wu V, Schulte J, Hirschi A, Tepass U, Beitel G. Sinuous is a Drosophila claudin required for septate junction organization and epithelial tube size control. J Cell Biol. 2004;164:313-23 pubmed
product information
Internal ID :
6445
Name :
DLG1
Depositor Name :
Nonet, M.L. / Hadwiger, G. / Dour, S.
Depositor Institution :
Washington University Medical School
Date Deposited :
2/16/10
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
DLG-1 (Discs Large)
Antigen Species :
C. elegans
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
C. elegans
Species Tested (legacy) :
C. elegans
Initial Publication Pubmed ID :
20405020
Depositor Notes (Special Instructions) :
Works well on C. elegans whole mounts.
Collections :
C. elegans
Search Keywords :
Nonet, M.L. / Hadwiger, G. / Dour, S., DLG-1 (Discs Large), C. elegans, MIgG1, C. elegans, dlg-1, , AB_2617529, monoclonal, C. elegans, Immunofluorescence/Western Blot
Antigen Molecular Weight :
Predicted: 107 kDa; Apparent: 107 kDa
Gene :
dlg-1
Uniprot ID :
G5ECY0
Antibody Registry ID :
AB_2617529
Synonyms (Alternate Clone Names) :
5E2
Immunogen :
Recombinant His6-tagged DLG-1 (aa 204-593) fusion protein
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Myeloma Strain :
P3X63Ag8.653
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 204-593, PDZ domains 1 to 3
Epitope Map PubMed ID :
20405020
Recommended Applications :
Immunofluorescence,Western Blot
Immunoblotting (legacy) :
good
Immunohistochemistry Pubmed IDs :
31353315 31412248
Immunofluorescence Pubmed IDs :
20405020 22535410
Western Blot Pubmed IDs :
20405020 27723735 21697505 23458156
Additional Information :
This antigen is the C. elegans homolog of the Drosophila membrane-associated guanylate kinase (MAGUK) family of scaffolding protein discs large protein, after which DLG-1 is named. In C. elegans, DLG-1 is primarily associated with the adherens junctions.
DSHB Growth Medium :
Iscove's
References (legacy) :
Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. (2010) A monoclonal antibody toolkit for C. elegans. PLoS One. 5(4):e10161-10115.; Cell 141, 509-523.; Dev. Biol. 343, 18-27
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US