ecdysone receptor (EcR common) | Developmental Studies Hybridoma Bank DDA2.7(EcR common) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

ecdysone receptor (EcR common)

catalog :

DDA2.7(EcR common)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DDA2.7(EcR common)

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 22

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:10; tbl 1	Alvarez Rivero J, Moris Sanz M, Estacio Gómez A, Montoliu Nerin M, Diaz Benjumea F, Herrero P. Variability in the number of abdominal leucokinergic neurons in adult Drosophila melanogaster. J Comp Neurol. 2017;525:639-660 pubmed publisher
immunocytochemistry; fruit fly; fig 5 western blot; fruit fly; 1:250; fig 6	Xie X, Hsu F, Gao X, Xu W, Ni J, Xing Y, et al. CDK8-Cyclin C Mediates Nutritional Regulation of Developmental Transitions through the Ecdysone Receptor in Drosophila. PLoS Biol. 2015;13:e1002207 pubmed publisher
	Sap K, Bezstarosti K, Dekkers D, van den Hout M, van Ijcken W, Rijkers E, et al. Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster. Proteomics. 2015;15:725-38 pubmed publisher
	Li Y, Ma Q, Cherry C, MATUNIS E. Steroid signaling promotes stem cell maintenance in the Drosophila testis. Dev Biol. 2014;394:129-41 pubmed publisher
	Schwedes C, Tulsiani S, Carney G. Ecdysone receptor expression and activity in adult Drosophila melanogaster. J Insect Physiol. 2011;57:899-907 pubmed publisher
	Francis V, Zorzano A, Teleman A. dDOR is an EcR coactivator that forms a feed-forward loop connecting insulin and ecdysone signaling. Curr Biol. 2010;20:1799-808 pubmed publisher
	van der Knaap J, Kozhevnikova E, Langenberg K, Moshkin Y, Verrijzer C. Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes. Mol Cell Biol. 2010;30:736-44 pubmed publisher
	Braun S, Azoitei A, Spindler Barth M. DNA-binding properties of Drosophila ecdysone receptor isoforms and their modification by the heterodimerization partner ultraspiracle. Arch Insect Biochem Physiol. 2009;72:172-91 pubmed publisher
	Beatty J, Fauth T, Callender J, Spindler Barth M, Henrich V. Analysis of transcriptional activity mediated by Drosophila melanogaster ecdysone receptor isoforms in a heterologous cell culture system. Insect Mol Biol. 2006;15:785-95 pubmed
	Sawatsubashi S, Maki A, Ito S, Shirode Y, Suzuki E, Zhao Y, et al. Ecdysone receptor-dependent gene regulation mediates histone poly(ADP-ribosyl)ation. Biochem Biophys Res Commun. 2004;320:268-72 pubmed
	Sedkov Y, Cho E, Petruk S, Cherbas L, Smith S, Jones R, et al. Methylation at lysine 4 of histone H3 in ecdysone-dependent development of Drosophila. Nature. 2003;426:78-83 pubmed
	Schubiger M, Tomita S, Sung C, Robinow S, Truman J. Isoform specific control of gene activity in vivo by the Drosophila ecdysone receptor. Mech Dev. 2003;120:909-18 pubmed
	Mouillet J, Henrich V, Lezzi M, Vogtli M. Differential control of gene activity by isoforms A, B1 and B2 of the Drosophila ecdysone receptor. Eur J Biochem. 2001;268:1811-9 pubmed
	Li T, Bender M. A conditional rescue system reveals essential functions for the ecdysone receptor (EcR) gene during molting and metamorphosis in Drosophila. Development. 2000;127:2897-905 pubmed
	Buszczak M, Freeman M, Carlson J, Bender M, Cooley L, Segraves W. Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila. Development. 1999;126:4581-9 pubmed
	Hodin J, Riddiford L. The ecdysone receptor and ultraspiracle regulate the timing and progression of ovarian morphogenesis during Drosophila metamorphosis. Dev Genes Evol. 1998;208:304-17 pubmed
	Bender M, Imam F, Talbot W, Ganetzky B, Hogness D. Drosophila ecdysone receptor mutations reveal functional differences among receptor isoforms. Cell. 1997;91:777-88 pubmed
	Antoniewski C, O Grady M, Edmondson R, Lassieur S, Benes H. Characterization of an EcR/USP heterodimer target site that mediates ecdysone responsiveness of the Drosophila Lsp-2 gene. Mol Gen Genet. 1995;249:545-56 pubmed
	Talbot W, Swyryd E, Hogness D. Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms. Cell. 1993;73:1323-37 pubmed
	Robinow S, Talbot W, Hogness D, Truman J. Programmed cell death in the Drosophila CNS is ecdysone-regulated and coupled with a specific ecdysone receptor isoform. Development. 1993;119:1251-9 pubmed
	Truman J, Talbot W, Fahrbach S, Hogness D. Ecdysone receptor expression in the CNS correlates with stage-specific responses to ecdysteroids during Drosophila and Manduca development. Development. 1994;120:219-34 pubmed
	Antoniewski C, Laval M, Dahan A, Lepesant J. The ecdysone response enhancer of the Fbp1 gene of Drosophila melanogaster is a direct target for the EcR/USP nuclear receptor. Mol Cell Biol. 1994;14:4465-74 pubmed

product information

Internal ID :

832

Name :

DDA2.7(EcR common)

Depositor Name :

Thummel, C. / Hogness, D.

Depositor Institution :

University of Utah

Depositor Website :

http://thummel.genetics.utah.edu/

Date Deposited :

11/1/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

ecdysone receptor (EcR common)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Amblyomma hebraeum,Drosophila,Lobster

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8324824

Collections :

Cell signaling,Drosophila antigens,Neurodevelopment,Transcription factors

Search Keywords :

Thummel, C. / Hogness, D., ecdysone receptor (EcR common), Drosophila, MIgG1, kappa light chain, Drosophila/Amblyomma hebraeum/Lobster, EcR, NR1H1, AB_10683834, monoclonal, Cell signaling/Drosophila antigens/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/Chromatin Immunoprecipitation/Gel Supershift

Antigen Molecular Weight :

Predicted: 91 kDa (EcR-A), 94 kDa (EcR-B1), 73 kDa (EcR-B2); Apparent; 105 kDa (EcR-A and EcR-B1) and 80 kDa (EcR-B2)

Gene :

EcR

Alternate Gene Name(s) :

NR1H1

Uniprot ID :

P34021

Antibody Registry ID :

AB_10683834

Immunogen :

TrpE-EcR-B1 fusion protein containing both isoform-specific and common segments of EcR-Bl (residues 68-878).

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 335-393

Epitope Map PubMed ID :

8324824

Recommended Applications :

Chromatin Immunoprecipitation,Gel Supershift,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8324824 21507325 8119129

Immunofluorescence Pubmed IDs :

8324824 10498692 19750549 25093968 9413987 8119129 9716721 12963111

Western Blot Pubmed IDs :

8324824 19995917 10851134 14603321 11248701 9716721 17201771 20888228

Immunoprecipitation Pubmed IDs :

25403936 14603321

Chromatin Immunoprecipitation Pubmed IDs :

15207731

Gel Supershift Pubmed IDs :

8007953 8544820

Pubmed IDs :

8306887

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 73, 1323-1337.

more info or order :

Developmental Studies Hybridoma Bank product webpage