cysteine string protein (CSP), Drosophila C-terminal | Developmental Studies Hybridoma Bank DCSP-3 (1G12) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

cysteine string protein (CSP), Drosophila C-terminal

catalog :

DCSP-3 (1G12)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCSP-3 (1G12)

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Reference
Koch N, Kobler O, Thomas U, Qualmann B, Kessels M. Terminal axonal arborization and synaptic bouton formation critically rely on abp1 and the arp2/3 complex. PLoS ONE. 2014;9:e97692 pubmed publisher
Lee M, Jang S, Nahm M, Yoon J, Lee S. Tbc1d15-17 regulates synaptic development at the Drosophila neuromuscular junction. Mol Cells. 2013;36:163-8 pubmed publisher
Dimitroff B, Howe K, Watson A, Campion B, Lee H, Zhao N, et al. Diet and energy-sensing inputs affect TorC1-mediated axon misrouting but not TorC2-directed synapse growth in a Drosophila model of tuberous sclerosis. PLoS ONE. 2012;7:e30722 pubmed publisher
Sarthi J, Elefant F. dTip60 HAT activity controls synaptic bouton expansion at the Drosophila neuromuscular junction. PLoS ONE. 2011;6:e26202 pubmed publisher
Fei H, Chow D, Chen A, Romero Calderon R, Ong W, Ackerson L, et al. Mutation of the Drosophila vesicular GABA transporter disrupts visual figure detection. J Exp Biol. 2010;213:1717-30 pubmed publisher
Johnson E, Fetter R, Davis G. Negative regulation of active zone assembly by a newly identified SR protein kinase. PLoS Biol. 2009;7:e1000193 pubmed publisher
Lee M, Paik S, Lee M, Kim Y, Kim S, Nahm M, et al. Drosophila Atlastin regulates the stability of muscle microtubules and is required for synapse development. Dev Biol. 2009;330:250-62 pubmed publisher
Arnold C, Reisch N, Leibold C, Becker S, Prüfert K, Sautter K, et al. Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus. J Exp Biol. 2004;207:1323-34 pubmed
Eberle K, Zinsmaier K, Buchner S, Gruhn M, Jenni M, Arnold C, et al. Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis. Cell Tissue Res. 1998;294:203-17 pubmed
Zinsmaier K, Eberle K, Buchner E, Walter N, Benzer S. Paralysis and early death in cysteine string protein mutants of Drosophila. Science. 1994;263:977-80 pubmed

product information

Internal ID :

915

Name :

DCSP-3 (1G12)

Depositor Name :

Benzer, S.

Depositor Institution :

California Institute of Technology

Date Deposited :

4/27/05

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

cysteine string protein (CSP), Drosophila C-terminal

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8310297

Collections :

Cell markers,Drosophila antigens,Neurodevelopment

Search Keywords :

Benzer, S., cysteine string protein (CSP), Drosophila C-terminal, Drosophila, MIgG2a, Drosophila, Csp, CG6395, AB_528184, monoclonal, Drosophila antigens/Neurodevelopment/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-3: 33 and 36 kDa.

Gene :

Csp

Alternate Gene Name(s) :

CG6395

Uniprot ID :

Q03751

Antibody Registry ID :

AB_528184

Immunogen :

Recombinant CSP/GST fusion protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

YTPDMVNQKY, aa 239-249 of isoform 1 (Uniprot ID: Q03751-1).

Epitope Map PubMed ID :

15010483

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8310297 9799436

Immunofluorescence Pubmed IDs :

23812537 22319582 22046262 19771148 19341724 20435823 24841972

Western Blot Pubmed IDs :

8310297 9799436 20435823

Additional Information :

This antibody will not recognize some isoforms that show a variant C-terminal like splicing isoform 3 (Uniprot ID: Q03751-3), and preferentially labels synapses of photoreceptors. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. CSP is also known as DnaJ homolog subfamily C member 5 homolog.

DSHB Growth Medium :

HL-1

References (legacy) :

Science 263, 977-980.

more info or order :

Developmental Studies Hybridoma Bank product webpage