product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
cysteine string protein (CSP), Drosophila common
catalog :
DCSP-2 (6D6)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DCSP-2 (6D6)
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 20
Published Application/Species/Sample/Dilution | Reference |
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Huang Y, Huang S, Lam S, Liu Z, Shui G, Zhang Y. Acsl, the Drosophila ortholog of intellectual-disability-related ACSL4, inhibits synaptic growth by altered lipids. J Cell Sci. 2016;129:4034-4045 pubmed
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Stowers R, Isacoff E. Drosophila huntingtin-interacting protein 14 is a presynaptic protein required for photoreceptor synaptic transmission and expression of the palmitoylated proteins synaptosome-associated protein 25 and cysteine string protein. J Neurosci. 2007;27:12874-83 pubmed
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Zeng X, Sun M, Liu L, Chen F, Wei L, Xie W. Neurexin-1 is required for synapse formation and larvae associative learning in Drosophila. FEBS Lett. 2007;581:2509-16 pubmed
| |
Serpe M, O Connor M. The metalloprotease tolloid-related and its TGF-beta-like substrate Dawdle regulate Drosophila motoneuron axon guidance. Development. 2006;133:4969-79 pubmed
| |
Arnold C, Reisch N, Leibold C, Becker S, Prüfert K, Sautter K, et al. Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus. J Exp Biol. 2004;207:1323-34 pubmed
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Eberle K, Zinsmaier K, Buchner S, Gruhn M, Jenni M, Arnold C, et al. Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis. Cell Tissue Res. 1998;294:203-17 pubmed
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Zinsmaier K, Eberle K, Buchner E, Walter N, Benzer S. Paralysis and early death in cysteine string protein mutants of Drosophila. Science. 1994;263:977-80 pubmed
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product information
Internal ID :
903
Name :
DCSP-2 (6D6)
Depositor Name :
Benzer, S.
Depositor Institution :
California Institute of Technology
Date Deposited :
4/27/05
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
cysteine string protein (CSP), Drosophila common
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
8310297
Collections :
Cell markers,Drosophila antigens,Neurodevelopment
Search Keywords :
Benzer, S., cysteine string protein (CSP), Drosophila common, Drosophila, MIgG2b, Drosophila, Csp, CG6395, AB_528183, monoclonal, Drosophila antigens/Neurodevelopment/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-2: 32, 33, 34, and 36 kDa.
Gene :
Csp
Alternate Gene Name(s) :
CG6395
Uniprot ID :
Q03751
Antibody Registry ID :
AB_528183
Immunogen :
Recombinant CSP/GST fusion protein
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Epitope Mapped :
Yes
Epitope Location or Sequence :
REQAGGQP, aa 218-222 of isoform 1 (Uniprot ID: Q03751-1).
Epitope Map PubMed ID :
15010483
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
8310297 9799436 21791202
Immunofluorescence Pubmed IDs :
24297851 17119021 18032660 24553921 22037499 20368622 22543973 21881207 20935173 27512046 27232889 27656110 22593074 23840848 25855186 30610177
Western Blot Pubmed IDs :
8310297 9799436 15010483 17498701 24553921
Additional Information :
This antibody recognizes all known CSP isoforms and labels all synapses. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. DCSP-2 is often used as a synaptic vesicle marker. CSP is also known as DnaJ homolog subfamily C member 5 homolog.
DSHB Growth Medium :
HL-1
References (legacy) :
Science 263, 977-980.; J. Neurosci. 32(20), 7058-7073.; PLoS ONE 8(6), e68324. Doi:10.1371/journal.pone.0068324.
more info or order :
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