cysteine string protein (CSP), Drosophila common | Developmental Studies Hybridoma Bank DCSP-1 (ab49) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

cysteine string protein (CSP), Drosophila common

catalog :

DCSP-1 (ab49)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCSP-1 (ab49)

reactivity :

Drosophila melanogaster

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; Drosophila melanogaster; 1:20; loading ...; fig 1b, 1c western blot; Drosophila melanogaster; 1:250; loading ...; fig 1s1c	Imler E, Pyon J, Kindelay S, Torvund M, Zhang Y, Chandra S, et al. A Drosophila model of neuronal ceroid lipofuscinosis CLN4 reveals a hypermorphic gain of function mechanism. elife. 2019;8: pubmed publisher
	Kleber J, Chen Y, Michels B, Saumweber T, Schleyer M, KÃ¤hne T, et al. Synapsin is required to "boost" memory strength for highly salient events. Learn Mem. 2016;23:9-20 pubmed publisher
	Dutta S, Rieche F, Eckl N, Duch C, Kretzschmar D. Glial expression of Swiss cheese (SWS), the Drosophila orthologue of neuropathy target esterase (NTE), is required for neuronal ensheathment and function. Dis Model Mech. 2016;9:283-94 pubmed publisher
	Beck K, Ehmann N, Andlauer T, Ljaschenko D, Strecker K, Fischer M, et al. Loss of the Coffin-Lowry syndrome-associated gene RSK2 alters ERK activity, synaptic function and axonal transport in Drosophila motoneurons. Dis Model Mech. 2015;8:1389-400 pubmed publisher
	Binotti B, Pavlos N, Riedel D, Wenzel D, VorbrÃ¼ggen G, Schalk A, et al. The GTPase Rab26 links synaptic vesicles to the autophagy pathway. elife. 2015;4:e05597 pubmed publisher
	Depner H, LÃ¼tzkendorf J, Babkir H, Sigrist S, Holt M. Differential centrifugation-based biochemical fractionation of the Drosophila adult CNS. Nat Protoc. 2014;9:2796-808 pubmed publisher
	Vanlandingham P, Barmchi M, ROYER S, Green R, Bao H, REIST N, et al. AP180 couples protein retrieval to clathrin-mediated endocytosis of synaptic vesicles. Traffic. 2014;15:433-50 pubmed publisher
	Kim M, Park H, Park H, Ro S, Nam S, Reed J, et al. Drosophila Fip200 is an essential regulator of autophagy that attenuates both growth and aging. Autophagy. 2013;9:1201-13 pubmed publisher
	Hofbauer A, Ebel T, Waltenspiel B, Oswald P, Chen Y, Halder P, et al. The Wuerzburg hybridoma library against Drosophila brain. J Neurogenet. 2009;23:78-91 pubmed publisher
	Bao H, Berlanga M, Xue M, Hapip S, Daniels R, Mendenhall J, et al. The atypical cadherin flamingo regulates synaptogenesis and helps prevent axonal and synaptic degeneration in Drosophila. Mol Cell Neurosci. 2007;34:662-78 pubmed
	Wagner S, Heseding C, Szlachta K, True J, Prinz H, Hovemann B. Drosophila photoreceptors express cysteine peptidase tan. J Comp Neurol. 2007;500:601-11 pubmed
	Rister J, Heisenberg M. Distinct functions of neuronal synaptobrevin in developing and mature fly photoreceptors. J Neurobiol. 2006;66:1271-84 pubmed
	Daniels R, Collins C, Gelfand M, Dant J, Brooks E, Krantz D, et al. Increased expression of the Drosophila vesicular glutamate transporter leads to excess glutamate release and a compensatory decrease in quantal content. J Neurosci. 2004;24:10466-74 pubmed
	Arnold C, Reisch N, Leibold C, Becker S, Prüfert K, Sautter K, et al. Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus. J Exp Biol. 2004;207:1323-34 pubmed
	Botella J, Kretzschmar D, Kiermayer C, Feldmann P, Hughes D, Schneuwly S. Deregulation of the Egfr/Ras signaling pathway induces age-related brain degeneration in the Drosophila mutant vap. Mol Biol Cell. 2003;14:241-50 pubmed
	Bronk P, Wenniger J, Dawson Scully K, Guo X, Hong S, Atwood H, et al. Drosophila Hsc70-4 is critical for neurotransmitter exocytosis in vivo. Neuron. 2001;30:475-88 pubmed
	Savvateeva E, Popov A, Kamyshev N, Bragina J, Heisenberg M, Senitz D, et al. Age-dependent memory loss, synaptic pathology and altered brain plasticity in the Drosophila mutant cardinal accumulating 3-hydroxykynurenine. J Neural Transm (Vienna). 2000;107:581-601 pubmed
	Eberle K, Zinsmaier K, Buchner S, Gruhn M, Jenni M, Arnold C, et al. Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis. Cell Tissue Res. 1998;294:203-17 pubmed
	Zinsmaier K, Eberle K, Buchner E, Walter N, Benzer S. Paralysis and early death in cysteine string protein mutants of Drosophila. Science. 1994;263:977-80 pubmed
	Zinsmaier K, Hofbauer A, Heimbeck G, Pflugfelder G, Buchner S, Buchner E. A cysteine-string protein is expressed in retina and brain of Drosophila. J Neurogenet. 1990;7:15-29 pubmed

product information

Internal ID :

7284

Name :

DCSP-1 (ab49)

Depositor Name :

Buchner, E. / Hofbauer, A.

Depositor Institution :

University of Wuerzburg

Date Deposited :

5/2/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

cysteine string protein (CSP), Drosophila common

Antigen Species :

Drosophila melanogaster

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

2129171

Depositor Notes (Special Instructions) :

Stains all synaptic neuropils and synaptic butons.

Collections :

Cell markers,Drosophila antigens,Neurodevelopment

Search Keywords :

Buchner, E. / Hofbauer, A., cysteine string protein (CSP), Drosophila common, Drosophila melanogaster, MIgG1, Drosophila, Csp, CG6395, AB_2307345, monoclonal, Drosophila antigens/Neurodevelopment/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-1: 32, 33, 34, and 36 kDa.

Gene :

Csp

Alternate Gene Name(s) :

CG6395

Uniprot ID :

Q03751

Antibody Registry ID :

AB_2307345

Immunogen :

Brain homogenate

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3X.63-Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

FTGA, aa 196-203 of isoform 1 (Uniprot ID: Q03751-1).

Epitope Map PubMed ID :

15010483

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

2129171 9799436 15010483 19132598 12529440 11072753 16967508 8310297

Immunofluorescence Pubmed IDs :

26398944 25643395 24456281 23819996 15548661 17321750 11395008 16967508

Western Blot Pubmed IDs :

2129171 9799436 15010483 26634819 26670182 24456281 25393777 12529440 17154266 11395008

Immunoprecipitation Pubmed IDs :

11395008

Additional Information :

This antibody recognizes all known CSP isoforms. CSP is associated with the cytoplasmic surface of synaptic vesicles and is often used as a synaptic vesicle marker. It labels all synapses. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. CSP is also known as DnaJ homolog subfamily C member 5 homolog.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Neurogenet. 7(1), 15-29.; Science 263, 981-982.; J. Exp. Biol. 207, 1323-1334.; J. Neurogenet. 23(1-2), 78-91.

more info or order :

Developmental Studies Hybridoma Bank product webpage