product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
cadherin, DE- (extracellular domain)
catalog :
DCAD2
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
DCAD2
reactivity :
dogs, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - free floating section
citations: 149
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; fruit fly ; loading ...; fig 1a-1c
Chen Y, Kotian N, Aranjuez G, Chen L, Messer C, Burtscher A, et al. Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration. elife. 2020;9: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:500; loading ...; fig 2b
Smith S, Davidson L, Rebeiz M. Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure. elife. 2020;9: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:20; loading ...; fig 2c
Kondo T, Hayashi S. Two-step regulation of trachealess ensures tight coupling of cell fate with morphogenesis in the Drosophila trachea. elife. 2019;8: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; loading ...; fig 5a
Cosolo A, Jaiswal J, Csordás G, Grass I, Uhlirova M, Classen A. JNK-dependent cell cycle stalling in G2 promotes survival and senescence-like phenotypes in tissue stress. elife. 2019;8: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:20; loading ...; fig s3c
Hakes A, Otsuki L, Brand A. A newly discovered neural stem cell population is generated by the optic lobe neuroepithelium during embryogenesis in Drosophila melanogaster. Development. 2018;145: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:3; loading ...; fig 7c
Lai C, Lin K, Kao S, Chen Y, Huang F, Hsu H. Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion. J Cell Biol. 2017;216:1439-1453 pubmed publisher
  • immunocytochemistry; dogs; 1:200; loading ...; fig s3
Gloerich M, Bianchini J, Siemers K, Cohen D, Nelson W. Cell division orientation is coupled to cell-cell adhesion by the E-cadherin/LGN complex. Nat Commun. 2017;8:13996 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:50; loading ...; fig s3d
Monyak R, Emerson D, Schoenfeld B, Zheng X, Chambers D, Rosenfelt C, et al. Insulin signaling misregulation underlies circadian and cognitive deficits in a Drosophila fragile X model. Mol Psychiatry. 2017;22:1140-1148 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:333; loading ...; fig 2c
Ducuing A, Vincent S. The actin cable is dispensable in directing dorsal closure dynamics but neutralizes mechanical stress to prevent scarring in the Drosophila embryo. Nat Cell Biol. 2016;18:1149-1160 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:500; fig s6
Liu M, Li Y, Liu A, Li R, Su Y, Du J, et al. The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development. elife. 2016;5: pubmed publisher
  • immunohistochemistry - paraffin section; fruit fly ; 1:50; fig 6
Woichansky I, Beretta C, Berns N, Riechmann V. Three mechanisms control E-cadherin localization to the zonula adherens. Nat Commun. 2016;7:10834 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:20; loading ...; tbl 3
Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; fig 1
Sun S, Reddy B, Irvine K. Localization of Hippo signalling complexes and Warts activation in vivo. Nat Commun. 2015;6:8402 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; fig 5
Campbell K, Casanova J. A role for E-cadherin in ensuring cohesive migration of a heterogeneous population of non-epithelial cells. Nat Commun. 2015;6:7998 pubmed publisher
  • immunohistochemistry - free floating section; fruit fly ; 1:20; fig 1
Francis D, Ghabrial A. Compensatory branching morphogenesis of stalk cells in the Drosophila trachea. Development. 2015;142:2048-57 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:250
SHERRARD K, Fehon R. The transmembrane protein Crumbs displays complex dynamics during follicular morphogenesis and is regulated competitively by Moesin and aPKC. Development. 2015;142:1869-78 pubmed publisher
  • immunohistochemistry - paraffin section; fruit fly ; 1:50
Bras Pereira C, Casares F, Janody F. The retinal determination gene Dachshund restricts cell proliferation by limiting the activity of the Homothorax-Yorkie complex. Development. 2015;142:1470-9 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:20
Fairchild M, Smendziuk C, Tanentzapf G. A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis. Development. 2015;142:268-81 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; fig 3
Castanieto A, Johnston M, Nystul T. EGFR signaling promotes self-renewal through the establishment of cell polarity in Drosophila follicle stem cells. elife. 2014;3: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:25
Djiane A, Zaessinger S, Babaoglan A, Bray S. Notch inhibits Yorkie activity in Drosophila wing discs. PLoS ONE. 2014;9:e106211 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; fig 3
Bosch J, Sumabat T, Hafezi Y, Pellock B, Gandhi K, Hariharan I. The Drosophila F-box protein Fbxl7 binds to the protocadherin fat and regulates Dachs localization and Hippo signaling. elife. 2014;3:e03383 pubmed publisher
  • immunocytochemistry; fruit fly ; 1:100
Piñeiro C, Lopes C, Casares F. A conserved transcriptional network regulates lamina development in the Drosophila visual system. Development. 2014;141:2838-47 pubmed publisher
  • immunocytochemistry; fruit fly ; 1:100
Lee J, Fischer J. Drosophila Tel2 is expressed as a translational fusion with EpsinR and is a regulator of wingless signaling. PLoS ONE. 2012;7:e46357 pubmed publisher
Plazaola Sasieta H, Zhu Q, Gaitán Peñas H, Rios M, Estévez R, Morey M. Drosophila ClC-a is required in glia of the stem cell niche for proper neurogenesis and wiring of neural circuits. Glia. 2019;67:2374-2398 pubmed publisher
Bäumers M, Klose S, Brüser C, Haag C, Hänsch S, Pannen H, et al. The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms. Biol Open. 2019;8: pubmed publisher
Thuveson M, Gaengel K, Collu G, Chin M, Singh J, Mlodzik M. Integrins are required for synchronous ommatidial rotation in the Drosophila eye linking planar cell polarity signalling to the extracellular matrix. Open Biol. 2019;9:190148 pubmed publisher
Nunes de Almeida F, Walther R, Pressé M, Vlassaks E, Pichaud F. Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs. Development. 2019;146: pubmed publisher
Uechi H, Kuranaga E. The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension. Dev Cell. 2019;50:327-338.e5 pubmed publisher
Letizia A, He D, Astigarraga S, Colombelli J, Hatini V, Llimargas M, et al. Sidekick Is a Key Component of Tricellular Adherens Junctions that Acts to Resolve Cell Rearrangements. Dev Cell. 2019;50:313-326.e5 pubmed publisher
Sharma S, Ghosh S, Geetha A, Mandal S, Mandal L. Cell Adhesion-Mediated Actomyosin Assembly Regulates the Activity of Cubitus Interruptus for Hematopoietic Progenitor Maintenance in Drosophila. Genetics. 2019;: pubmed publisher
Pütz S. Mbt/PAK4 together with SRC modulates N-Cadherin adherens junctions in the developing Drosophila eye. Biol Open. 2019;8: pubmed publisher
Nakato E, Liu X, Eriksson I, Yamamoto M, Kinoshita Toyoda A, Toyoda H, et al. Establishment and characterization of Drosophila cell lines mutant for heparan sulfate modifying enzymes. Glycobiology. 2019;29:479-489 pubmed publisher
Bertran M, Mouilleron S, Zhou Y, Bajaj R, Uliana F, Kumar G, et al. ASPP proteins discriminate between PP1 catalytic subunits through their SH3 domain and the PP1 C-tail. Nat Commun. 2019;10:771 pubmed publisher
Moreno E, Valon L, Levillayer F, Levayer R. Competition for Space Induces Cell Elimination through Compaction-Driven ERK Downregulation. Curr Biol. 2019;29:23-34.e8 pubmed publisher
Vuong L, Iomini C, Balmer S, Esposito D, Aaronson S, Mlodzik M. Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling. Nat Commun. 2018;9:5304 pubmed publisher
Sui L, Alt S, Weigert M, Dye N, Eaton S, Jug F, et al. Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms. Nat Commun. 2018;9:4620 pubmed publisher
White K, Grillo Hill B, Esquivel M, Peralta J, Bui V, Chire I, et al. β-Catenin is a pH sensor with decreased stability at higher intracellular pH. J Cell Biol. 2018;217:3965-3976 pubmed publisher
Ansar M, Chung H, Taylor R, Nazir A, Imtiaz S, Sarwar M, et al. Bi-allelic Loss-of-Function Variants in DNMBP Cause Infantile Cataracts. Am J Hum Genet. 2018;103:568-578 pubmed publisher
Courgeon M, He D, Liu H, Legent K, Treisman J. The Drosophila Epidermal Growth Factor Receptor does not act in the nucleus. J Cell Sci. 2018;131: pubmed publisher
Sawant K, Chen Y, Kotian N, Preuss K, McDonald J. Rap1 GTPase promotes coordinated collective cell migration in vivo. Mol Biol Cell. 2018;29:2656-2673 pubmed publisher
Öztürk Çolak A, Stephan Otto Attolini C, Casanova J, Araújo S. Blimp-1 Mediates Tracheal Lumen Maturation in Drosophila melanogaster. Genetics. 2018;210:653-663 pubmed publisher
Weaver L, Drummond Barbosa D. Maintenance of Proper Germline Stem Cell Number Requires Adipocyte Collagen in Adult Drosophila Females. Genetics. 2018;209:1155-1166 pubmed publisher
Fisher K, Fragiadaki M, Pugazhendhi D, Bausek N, Arredondo M, Thomas S, et al. A genome-wide RNAi screen identifies MASK as a positive regulator of cytokine receptor stability. J Cell Sci. 2018;131: pubmed publisher
Kline A, Curry T, Lewellyn L. The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber. Dev Biol. 2018;440:99-112 pubmed publisher
Ratheesh A, Biebl J, Veselá J, Smutny M, Papusheva E, Krens S, et al. Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration. Dev Cell. 2018;45:331-346.e7 pubmed publisher
Allbee A, Rincon Limas D, Biteau B. Lmx1a is required for the development of the ovarian stem cell niche in Drosophila. Development. 2018;145: pubmed publisher
Wang X, Page McCaw A. Wnt6 maintains anterior escort cells as an integral component of the germline stem cell niche. Development. 2018;145: pubmed publisher
Beati H, Peek I, Hordowska P, Honemann Capito M, Glashauser J, Renschler F, et al. The adherens junction-associated LIM domain protein Smallish regulates epithelial morphogenesis. J Cell Biol. 2018;217:1079-1095 pubmed publisher
Tsoumpekos G, Nemetschke L, Knust E. Drosophila Big bang regulates the apical cytocortex and wing growth through junctional tension. J Cell Biol. 2018;217:1033-1045 pubmed publisher
Forest E, Logeay R, Geminard C, Kantar D, Frayssinoux F, Heron Milhavet L, et al. The apical scaffold big bang binds to spectrins and regulates the growth of Drosophila melanogaster wing discs. J Cell Biol. 2018;217:1047-1062 pubmed publisher
Morrison C, Chen H, Cook T, Brown S, Treisman J. Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye. PLoS Genet. 2018;14:e1007173 pubmed publisher
Samarasekera G, Auld V. C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src. Mol Biol Cell. 2018;29:123-136 pubmed publisher
Rothenbusch Fender S, Fritzen K, Bischoff M, Buttgereit D, Oenel S, Renkawitz Pohl R. Myotube migration to cover and shape the testis of Drosophila depends on Heartless, Cadherin/Catenin, and myosin II. Biol Open. 2017;6:1876-1888 pubmed publisher
Dai W, Peterson A, Kenney T, Burrous H, Montell D. Quantitative microscopy of the Drosophila ovary shows multiple niche signals specify progenitor cell fate. Nat Commun. 2017;8:1244 pubmed publisher
O Farrell F, Lobert V, Sneeggen M, Jain A, Katheder N, Wenzel E, et al. Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation. Nat Cell Biol. 2017;19:1412-1423 pubmed publisher
Guichard A, Jain P, Moayeri M, Schwartz R, Chin S, Zhu L, et al. Anthrax edema toxin disrupts distinct steps in Rab11-dependent junctional transport. PLoS Pathog. 2017;13:e1006603 pubmed publisher
Ochoa Espinosa A, Harmansa S, Caussinus E, Affolter M. Myosin II is not required for Drosophila tracheal branch elongation and cell intercalation. Development. 2017;144:2961-2968 pubmed publisher
Muzzopappa M, Murcia L, Milan M. Feedback amplification loop drives malignant growth in epithelial tissues. Proc Natl Acad Sci U S A. 2017;114:E7291-E7300 pubmed publisher
LeBlanc M, Lehmann R. Domain-specific control of germ cell polarity and migration by multifunction Tre1 GPCR. J Cell Biol. 2017;216:2945-2958 pubmed publisher
Dahal G, Pradhan S, Bates E. Inwardly rectifying potassium channels influence Drosophila wing morphogenesis by regulating Dpp release. Development. 2017;144:2771-2783 pubmed publisher
Cook M, Cazin C, Amoyel M, Yamamoto S, Bach E, Nystul T. Neutral Competition for Drosophila Follicle and Cyst Stem Cell Niches Requires Vesicle Trafficking Genes. Genetics. 2017;206:1417-1428 pubmed publisher
Du L, Zhou A, Patel A, Rao M, Anderson K, Roy S. Unique patterns of organization and migration of FGF-expressing cells during Drosophila morphogenesis. Dev Biol. 2017;427:35-48 pubmed publisher
Perez Mockus G, Roca V, Mazouni K, Schweisguth F. Neuralized regulates Crumbs endocytosis and epithelium morphogenesis via specific Stardust isoforms. J Cell Biol. 2017;216:1405-1420 pubmed publisher
Poulton J, Cuningham J, Peifer M. Centrosome and spindle assembly checkpoint loss leads to neural apoptosis and reduced brain size. J Cell Biol. 2017;216:1255-1265 pubmed publisher
Doyle S, Pahl M, Siller K, Ardiff L, Siegrist S. Neuroblast niche position is controlled by Phosphoinositide 3-kinase-dependent DE-Cadherin adhesion. Development. 2017;144:820-829 pubmed publisher
Gómez J, Chumakova L, Bulgakova N, Brown N. Microtubule organization is determined by the shape of epithelial cells. Nat Commun. 2016;7:13172 pubmed publisher
Ma X, Han Y, Song X, Do T, Yang Z, Ni J, et al. DNA damage-induced Lok/CHK2 activation compromises germline stem cell self-renewal and lineage differentiation. Development. 2016;143:4312-4323 pubmed
Zhou Q, Neal S, Pignoni F. Mutant analysis by rescue gene excision: New tools for mosaic studies in Drosophila. Genesis. 2016;54:589-592 pubmed publisher
Tsao C, Ku H, Lee Y, Huang Y, Sun Y. Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs. PLoS ONE. 2016;11:e0163744 pubmed publisher
Tamori Y, Suzuki E, Deng W. Epithelial Tumors Originate in Tumor Hotspots, a Tissue-Intrinsic Microenvironment. PLoS Biol. 2016;14:e1002537 pubmed publisher
Michel M, Aliee M, Rudolf K, Bialas L, Jülicher F, Dahmann C. The Selector Gene apterous and Notch Are Required to Locally Increase Mechanical Cell Bond Tension at the Drosophila Dorsoventral Compartment Boundary. PLoS ONE. 2016;11:e0161668 pubmed publisher
Mason F, Xie S, Vasquez C, Tworoger M, Martin A. RhoA GTPase inhibition organizes contraction during epithelial morphogenesis. J Cell Biol. 2016;214:603-17 pubmed publisher
Padash Barmchi M, GILBERT M, Thomas M, Banks L, Zhang B, Auld V. A Drosophila Model of HPV E6-Induced Malignancy Reveals Essential Roles for Magi and the Insulin Receptor. PLoS Pathog. 2016;12:e1005789 pubmed publisher
Xie S, Mason F, Martin A. Loss of G?12/13 exacerbates apical area dependence of actomyosin contractility. Mol Biol Cell. 2016;27:3526-3536 pubmed
Bras Pereira C, Potier D, Jacobs J, Aerts S, Casares F, Janody F. dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. PLoS Genet. 2016;12:e1006204 pubmed publisher
Jodoin J, Martin A. Abl suppresses cell extrusion and intercalation during epithelium folding. Mol Biol Cell. 2016;27:2822-32 pubmed publisher
Ameku T, Niwa R. Mating-Induced Increase in Germline Stem Cells via the Neuroendocrine System in Female Drosophila. PLoS Genet. 2016;12:e1006123 pubmed publisher
He B, Martin A, Wieschaus E. Flow-dependent myosin recruitment during Drosophila cellularization requires zygotic dunk activity. Development. 2016;143:2417-30 pubmed publisher
Padash Barmchi M, Samarasekera G, GILBERT M, Auld V, Zhang B. Magi Is Associated with the Par Complex and Functions Antagonistically with Bazooka to Regulate the Apical Polarity Complex. PLoS ONE. 2016;11:e0153259 pubmed publisher
Walther R, Nunes de Almeida F, Vlassaks E, Burden J, Pichaud F. Pak4 Is Required during Epithelial Polarity Remodeling through Regulating AJ Stability and Bazooka Retention at the ZA. Cell Rep. 2016;15:45-53 pubmed publisher
Upadhyay M, Martino Cortez Y, Wong Deyrup S, Tavares L, Schowalter S, Flora P, et al. Transposon Dysregulation Modulates dWnt4 Signaling to Control Germline Stem Cell Differentiation in Drosophila. PLoS Genet. 2016;12:e1005918 pubmed publisher
Ng B, Selvaraj G, Santa Cruz Mateos C, Grosheva I, Alvarez Garcia I, Martín Bermudo M, et al. α-Spectrin and integrins act together to regulate actomyosin and columnarization, and to maintain a monolayered follicular epithelium. Development. 2016;143:1388-99 pubmed publisher
Hunter M, Lee D, Harris T, Fernandez Gonzalez R. Polarized E-cadherin endocytosis directs actomyosin remodeling during embryonic wound repair. J Cell Biol. 2015;210:801-16 pubmed publisher
Meserve J, Duronio R. Scalloped and Yorkie are required for cell cycle re-entry of quiescent cells after tissue damage. Development. 2015;142:2740-51 pubmed publisher
Wingert L, Dinardo S. Traffic jam functions in a branched pathway from Notch activation to niche cell fate. Development. 2015;142:2268-77 pubmed publisher
Manning L, Weideman A, Peercy B, Starz Gaiano M. Tissue landscape alters adjacent cell fates during Drosophila egg development. Nat Commun. 2015;6:7356 pubmed publisher
Grillo Hill B, Choi C, Jimenez Vidal M, Barber D. Increased H⁺ efflux is sufficient to induce dysplasia and necessary for viability with oncogene expression. elife. 2015;4: pubmed publisher
Shahab J, Tiwari M, Honemann Capito M, Krahn M, Wodarz A. Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells. Biol Open. 2015;4:528-41 pubmed publisher
Verboon J, Rahe T, Rodriguez Mesa E, Parkhurst S. Wash functions downstream of Rho1 GTPase in a subset of Drosophila immune cell developmental migrations. Mol Biol Cell. 2015;26:1665-74 pubmed publisher
Pert M, Gan M, Saint R, Murray M. Netrins and Frazzled/DCC promote the migration and mesenchymal to epithelial transition of Drosophila midgut cells. Biol Open. 2015;4:233-43 pubmed publisher
Jia Q, Liu Y, Liu H, Li S. Mmp1 and Mmp2 cooperatively induce Drosophila fat body cell dissociation with distinct roles. Sci Rep. 2014;4:7535 pubmed publisher
Goldenberg G, Harris T. Adherens junction distribution mechanisms during cell-cell contact elongation in Drosophila. PLoS ONE. 2013;8:e79613 pubmed publisher
Huelsmann S, Ylanne J, Brown N. Filopodia-like actin cables position nuclei in association with perinuclear actin in Drosophila nurse cells. Dev Cell. 2013;26:604-15 pubmed publisher
Giuliani F, Giuliani G, Bauer R, Rabouille C. Innexin 3, a new gene required for dorsal closure in Drosophila embryo. PLoS ONE. 2013;8:e69212 pubmed publisher
Bulgakova N, Grigoriev I, Yap A, Akhmanova A, Brown N. Dynamic microtubules produce an asymmetric E-cadherin-Bazooka complex to maintain segment boundaries. J Cell Biol. 2013;201:887-901 pubmed publisher
Lucas E, Khanal I, Gaspar P, Fletcher G, Polesello C, Tapon N, et al. The Hippo pathway polarizes the actin cytoskeleton during collective migration of Drosophila border cells. J Cell Biol. 2013;201:875-85 pubmed publisher
Talamillo A, Herboso L, Pirone L, P rez C, Gonz lez M, S nchez J, et al. Scavenger receptors mediate the role of SUMO and Ftz-f1 in Drosophila steroidogenesis. PLoS Genet. 2013;9:e1003473 pubmed publisher
Neisch A, Formstecher E, Fehon R. Conundrum, an ARHGAP18 orthologue, regulates RhoA and proliferation through interactions with Moesin. Mol Biol Cell. 2013;24:1420-33 pubmed publisher
Choi W, Harris N, Sumigray K, Peifer M. Rap1 and Canoe/afadin are essential for establishment of apical-basal polarity in the Drosophila embryo. Mol Biol Cell. 2013;24:945-63 pubmed publisher
Gómez J, Wang Y, Riechmann V. Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis. J Cell Biol. 2012;199:1131-43 pubmed publisher
Jeon M, Scott M, Zinn K. Interactions between Type III receptor tyrosine phosphatases and growth factor receptor tyrosine kinases regulate tracheal tube formation in Drosophila. Biol Open. 2012;1:548-58 pubmed publisher
Aranjuez G, Kudlaty E, Longworth M, McDonald J. On the role of PDZ domain-encoding genes in Drosophila border cell migration. G3 (Bethesda). 2012;2:1379-91 pubmed publisher
Sen A, Nagy Zsvér Vadas Z, Krahn M. Drosophila PATJ supports adherens junction stability by modulating Myosin light chain activity. J Cell Biol. 2012;199:685-98 pubmed publisher
Lim J, Fuller M. Somatic cell lineage is required for differentiation and not maintenance of germline stem cells in Drosophila testes. Proc Natl Acad Sci U S A. 2012;109:18477-81 pubmed publisher
Peng Y, Han C, Axelrod J. Planar polarized protrusions break the symmetry of EGFR signaling during Drosophila bract cell fate induction. Dev Cell. 2012;23:507-18 pubmed publisher
Reid D, Muyskens J, Neal J, Gaddini G, Cho L, Wandler A, et al. Identification of genetic modifiers of CagA-induced epithelial disruption in Drosophila. Front Cell Infect Microbiol. 2012;2:24 pubmed publisher
Chen D, Li M, Wu S, Lin Y, Tsai S, Lai P, et al. The Bro1-domain-containing protein Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration. J Cell Sci. 2012;125:4841-52 pubmed publisher
Yang N, Inaki M, Cliffe A, Rørth P. Microtubules and Lis-1/NudE/dynein regulate invasive cell-on-cell migration in Drosophila. PLoS ONE. 2012;7:e40632 pubmed publisher
Lin C, Lin P, Li Y, Hsu J. Capulet and Slingshot share overlapping functions during Drosophila eye morphogenesis. J Biomed Sci. 2012;19:46 pubmed publisher
Li Y, Padgett R. bantam is required for optic lobe development and glial cell proliferation. PLoS ONE. 2012;7:e32910 pubmed publisher
Daskalaki A, Shalaby N, Kux K, Tsoumpekos G, Tsibidis G, Muskavitch M, et al. Distinct intracellular motifs of Delta mediate its ubiquitylation and activation by Mindbomb1 and Neuralized. J Cell Biol. 2011;195:1017-31 pubmed publisher
Richter C, Oktaba K, Steinmann J, Muller J, Knoblich J. The tumour suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements. Nat Cell Biol. 2011;13:1029-39 pubmed publisher
VanZomeren Dohm A, Sarro J, Flannery E, Duman Scheel M. The Drosophila Netrin receptor frazzled/DCC functions as an invasive tumor suppressor. BMC Dev Biol. 2011;11:41 pubmed publisher
Mateus A, Gorfinkiel N, Schamberg S, Martinez Arias A. Endocytic and recycling endosomes modulate cell shape changes and tissue behaviour during morphogenesis in Drosophila. PLoS ONE. 2011;6:e18729 pubmed publisher
Abreu Blanco M, Verboon J, Parkhurst S. Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling. J Cell Biol. 2011;193:455-64 pubmed publisher
Djiane A, Shimizu H, Wilkin M, Mazleyrat S, Jennings M, Avis J, et al. Su(dx) E3 ubiquitin ligase-dependent and -independent functions of polychaetoid, the Drosophila ZO-1 homologue. J Cell Biol. 2011;192:189-200 pubmed publisher
Kim M, McGinnis W. Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier. Proc Natl Acad Sci U S A. 2011;108:650-5 pubmed publisher
Sun G, Irvine K. Regulation of Hippo signaling by Jun kinase signaling during compensatory cell proliferation and regeneration, and in neoplastic tumors. Dev Biol. 2011;350:139-51 pubmed publisher
Gandille P, Narbonne Reveau K, Boissonneau E, Randsholt N, Busson D, Pret A. Mutations in the polycomb group gene polyhomeotic lead to epithelial instability in both the ovary and wing imaginal disc in Drosophila. PLoS ONE. 2010;5:e13946 pubmed publisher
Chen S, Kaneko S, Ma X, Chen X, Ip Y, Xu L, et al. Lissencephaly-1 controls germline stem cell self-renewal through modulating bone morphogenetic protein signaling and niche adhesion. Proc Natl Acad Sci U S A. 2010;107:19939-44 pubmed publisher
Krahn M, Bückers J, Kastrup L, Wodarz A. Formation of a Bazooka-Stardust complex is essential for plasma membrane polarity in epithelia. J Cell Biol. 2010;190:751-60 pubmed publisher
Ueyama M, Akimoto Y, Ichimiya T, Ueda R, Kawakami H, Aigaki T, et al. Increased apoptosis of myoblasts in Drosophila model for the Walker-Warburg syndrome. PLoS ONE. 2010;5:e11557 pubmed publisher
Hilgers V, Bushati N, Cohen S. Drosophila microRNAs 263a/b confer robustness during development by protecting nascent sense organs from apoptosis. PLoS Biol. 2010;8:e1000396 pubmed publisher
Siekhaus D, Haesemeyer M, Moffitt O, Lehmann R. RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila. Nat Cell Biol. 2010;12:605-10 pubmed publisher
Morais de Sá E, Mirouse V, St Johnston D. aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells. Cell. 2010;141:509-23 pubmed publisher
Benhra N, Vignaux F, Dussert A, Schweisguth F, Le Borgne R. Neuralized promotes basal to apical transcytosis of delta in epithelial cells. Mol Biol Cell. 2010;21:2078-86 pubmed publisher
Neisch A, Speck O, Stronach B, Fehon R. Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. J Cell Biol. 2010;189:311-23 pubmed publisher
Maurel Zaffran C, Pradel J, Graba Y. Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis. Dev Biol. 2010;343:18-27 pubmed publisher
Campbell K, Casanova J, Skaer H. Mesenchymal-to-epithelial transition of intercalating cells in Drosophila renal tubules depends on polarity cues from epithelial neighbours. Mech Dev. 2010;127:345-57 pubmed publisher
Martin A, Gelbart M, Fernandez Gonzalez R, Kaschube M, Wieschaus E. Integration of contractile forces during tissue invagination. J Cell Biol. 2010;188:735-49 pubmed publisher
Pepper A, Beerman R, Bhogal B, Jongens T. Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS ONE. 2009;4:e7618 pubmed publisher
Kaido M, Wada H, Shindo M, Hayashi S. Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila. Genes Cells. 2009;14:1067-77 pubmed publisher
Demontis F, Dahmann C. Characterization of the Drosophila ortholog of the human Usher Syndrome type 1G protein sans. PLoS ONE. 2009;4:e4753 pubmed publisher
Rusan N, Akong K, Peifer M. Putting the model to the test: are APC proteins essential for neuronal polarity, axon outgrowth, and axon targeting?. J Cell Biol. 2008;183:203-12 pubmed publisher
Kunwar P, Sano H, Renault A, Barbosa V, Fuse N, Lehmann R. Tre1 GPCR initiates germ cell transepithelial migration by regulating Drosophila melanogaster E-cadherin. J Cell Biol. 2008;183:157-68 pubmed publisher
Somorjai I, Martinez Arias A. Wingless signalling alters the levels, subcellular distribution and dynamics of Armadillo and E-cadherin in third instar larval wing imaginal discs. PLoS ONE. 2008;3:e2893 pubmed publisher
Todi S, Sivan Loukianova E, Jacobs J, Kiehart D, Eberl D. Myosin VIIA, important for human auditory function, is necessary for Drosophila auditory organ development. PLoS ONE. 2008;3:e2115 pubmed publisher
Villa Cuesta E, González Pérez E, Modolell J. Apposition of iroquois expressing and non-expressing cells leads to cell sorting and fold formation in the Drosophila imaginal wing disc. BMC Dev Biol. 2007;7:106 pubmed
Clark I, Jarman A, Finnegan D. Live imaging of Drosophila gonad formation reveals roles for Six4 in regulating germline and somatic cell migration. BMC Dev Biol. 2007;7:52 pubmed
Saito K, Nishida K, Mori T, Kawamura Y, Miyoshi K, Nagami T, et al. Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome. Genes Dev. 2006;20:2214-22 pubmed
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product information
Internal ID :
880
Name :
DCAD2
Depositor Name :
Uemura, T.
Depositor Institution :
Kyoto Univ. Graduate Sch.of Biostudies
Date Deposited :
1/7/04
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
cadherin, DE- (extracellular domain)
Antigen Species :
Drosophila
Host Species :
rat
Isotype :
RIgG2a, kappa light chain
Isotype for catalog (legacy) :
IgG2a, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
7958432
Depositor Notes (Special Instructions) :
Suitable for use in immunostaining and immunoprecipitation. In western blot analysis, DCAD1, another rat monoclonal antibody to DE-cadherin, is more sensitive than DCAD2, although DCAD1 is not appropriate for staining. About requests of DCAD1, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). Conditions for immunostaining are described in our papers (Developmental Biology; Genes & Development 10, 659- 671); in addition please note the following comments. 1/ Fixatives (3.7% formaldehyde in PB or 4% paraformaldehyde in CMF & GBS containing 1mM CaCl2). Either fixation protocol works to stain embryos and discs. To stain cultured cells, only the 4% paraformaldehyde condition worked in our hands. We do not prefer any fix solutions including EDTA or EGTA, because cadherin is easily degraded under Ca++- free conditions. 2/ We suspect that the epitope may be sensitive to long exposure to alcohol. So, embryos stored in alcohol may not be stained. 3/ Photos should be taken under confocal microscope. 4/ HRP/DAB reaction has never given clear-cut signals for DE-cadherin at cell-cell contact sites.
Collections :
Cell adhesion,Cell markers,Drosophila antigens,Neurodevelopment
Search Keywords :
Uemura, T., cadherin, DE- (extracellular domain), Drosophila, RIgG2a, kappa light chain, Drosophila, shg, CG3722, E-cadh, AB_528120, monoclonal, Drosophila antigens/Cell adhesion/Neurodevelopment/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: 170 kDa; Apparent: 230 kDa [precursor] and 150 kDa [mature form]
Gene :
shg
Alternate Gene Name(s) :
CG3722, E-cadh
Uniprot ID :
Q24298
Antibody Registry ID :
AB_528120
Immunogen :
Proteins co-immunoprecipitated with an anti-alpha-catenin mAb, DCAT-1.
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
P3-X63-Ag8-U1
Epitope Mapped :
Yes
Epitope Location or Sequence :
Between cadherin repeats 0 and 1
Epitope Map PubMed ID :
10588889
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunohistochemistry Pubmed IDs :
25793441 31353316 31353315 31409231
Immunofluorescence Pubmed IDs :
20495554 20434988 20410139 20403348 21145886 21187384 22921201 8598294 16224820 10588889 10079365 19682089 21041636 12551956 22825871 16882972 27442438 27685172 27696669 29093440 28808034 28811312 29167383 29324767 29753016 29738712 30397306 30869121
Western Blot Pubmed IDs :
7958432 10588889 10409711 19682089 31353316
Immunoprecipitation Pubmed IDs :
7958432 9247265 10079365 12551956
Additional Information :
For experiments involving live cells and tissue, DCAD2 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.
DSHB Growth Medium :
Iscove's
References (legacy) :
Dev. Biol. 165, 716-726.; Nat. Cell Biol. 12, 605-610.; Cell 141, 509-523.; Dev. Biol. 343, 18-27.; Mole. Biol. Cell 21, 2078-2086.; Dev. Biol. 350, 139-151.; PNAS 108(2), 650-655.; Dev. Cell 23, 507-518.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US