DAO-5 | Developmental Studies Hybridoma Bank DAO5 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

DAO-5

catalog :

DAO5

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DAO5

reactivity :

Caenorhabditis elegans

application :

western blot, immunocytochemistry, chromatin immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 7

Reference
Narbonne P, Maddox P, Labbé J. DAF-18/PTEN locally antagonizes insulin signalling to couple germline stem cell proliferation to oocyte needs in C. elegans. Development. 2015;142:4230-41 pubmed publisher
Lee C, Tsai Y, Kao C, Lee L, Lai H, Ma T, et al. Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans. Cell Death Dis. 2014;5:e1158 pubmed publisher
Kershner A, Shin H, Hansen T, Kimble J. Discovery of two GLP-1/Notch target genes that account for the role of GLP-1/Notch signaling in stem cell maintenance. Proc Natl Acad Sci U S A. 2014;111:3739-44 pubmed publisher
Grün D, Kirchner M, Thierfelder N, Stoeckius M, Selbach M, Rajewsky N. Conservation of mRNA and protein expression during development of C. elegans. Cell Rep. 2014;6:565-77 pubmed publisher
Korčeková D, Gombitová A, Raska I, Cmarko D, Lanctôt C. Nucleologenesis in the Caenorhabditis elegans embryo. PLoS ONE. 2012;7:e40290 pubmed publisher
Cheng L, Li B, Wang Z. Level-Set Minimization of Potential Controlled Hadwiger Valuations for Molecular Solvation. J Comput Phys. 2010;229:8497-8510 pubmed
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher

product information

Internal ID :

6461

Name :

DAO5

Depositor Name :

Nonet, M.L. / Hadwiger, G. / Dour, S.

Depositor Institution :

Washington University Medical School

Date Deposited :

2/16/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

DAO-5

Antigen Species :

C. elegans

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b

Positive Tested Species Reactivity :

C. elegans

Species Tested (legacy) :

C. elegans

Initial Publication Pubmed ID :

20405020

Depositor Notes (Special Instructions) :

Works on C. elegans whole mounts. Stains the nucleosomes.

Collections :

C. elegans

Search Keywords :

Nonet, M.L. / Hadwiger, G. / Dour, S., DAO-5, C. elegans, MIgG2b, C. elegans, dao-5, , AB_10573805, monoclonal, C. elegans, Immunofluorescence/Western Blot/Chromatin Immunoprecipitation

Antigen Molecular Weight :

Predicted: 73 and 101 kDa; multiple bands from 10 to 150 kDa.

Gene :

dao-5

Uniprot ID :

Q9XVS4 Q564W7

Antibody Registry ID :

AB_10573805

Synonyms (Alternate Clone Names) :

5E9

Immunogen :

Recombinant His6-tagged DAO-5 (C-terminal 221 aa) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal 221 aa

Epitope Map PubMed ID :

20405020

Recommended Applications :

Chromatin Immunoprecipitation,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

20405020 22768349 24722283 24567412 26552888

Western Blot Pubmed IDs :

20405020 24462290 24722283

Chromatin Immunoprecipitation Pubmed IDs :

24722283

Additional Information :

DAO-5 is a homolog of the nucleolar phosphoprotein Nopp140. This protein has been reported to give multiple bands on western due to post-translational modification, proteolysis, dimerization, trimerization and alternative splicing.

DSHB Growth Medium :

Iscove's

References (legacy) :

Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. (2010) A monoclonal antibody toolkit for C. elegans. PLoS One. 5(4):e10161-10115.

more info or order :

Developmental Studies Hybridoma Bank product webpage