Desmin, intermediate filament | Developmental Studies Hybridoma Bank D76 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Desmin, intermediate filament

catalog :

D76

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D76

reactivity :

mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse	Can T, Faas L, Ashford D, Dowle A, Thomas J, O Toole P, et al. Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections. Proteome Sci. 2014;12:25 pubmed publisher

product information

Internal ID :

3082

Name :

D76

Depositor Name :

Fischman, D.A.

Depositor Institution :

Cornell University Medical College

Date Deposited :

4/22/86

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Desmin, intermediate filament

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chicken,Fish,Lamb,Mouse,Rat

Initial Publication Pubmed ID :

6373792

Depositor Notes (Special Instructions) :

The D76 monoclonal antibodies bind to desmin but recognize different epitopes in all types of muscle including vascular smooth muscle but is unreactive with embryonic cardiac myocytes. D76 also labels electric organs in fish and myoid cells in chick and fish thymus.

Collections :

Cytoskeleton,Human,Muscular dystrophy,Neurodevelopment,Skeletal muscle

Search Keywords :

Fischman, D.A., Desmin, intermediate filament, Chicken, MIgG1, Chicken/Mouse/Lamb/Fish/Rat, DES, , AB_528196, Monoclonal , cytoskeleton/Skeletal muscle, Western blot/Immunofluorescence/Immunohistochemistry/Immunoprecipitation

Antigen Molecular Weight :

53.2 kDa

Gene :

DES

Uniprot ID :

P02542

Antibody Registry ID :

AB_528196

Immunogen :

Acetic acid-solubilized desmin from gizzard

Immunogen Sequence :

Full length sequence

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

a.a. 97-255, probably near the N-terminus of Helix 1A

Epitope Map PubMed ID :

2450097

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Blots nicely

Immunohistochemistry Pubmed IDs :

6373792 25381569 19166477 8799862

Immunofluorescence Pubmed IDs :

25071420 17077280 9682974 9251254 8775982 2292359 6373792 2323957 29505887 12601693 25071420

Western Blot Pubmed IDs :

6373792 26702144 8655433 29726007 17077280 8799862

Immunoprecipitation Pubmed IDs :

27733623

Depositor Growth Medium :

DME supplemented only with 10 or 20% (vol/vol) fetal bovine or gamma globulin-free horse serum

Additional Information :

Isopentane-cooled frozen sections or Triton X-100 extracted cultured cells are recommended for immunostaining. RRID: AB_528196

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 98, 2179-2191.; Histochem 93, 423-428.; Differentiation 45, 21-28.; J. Tiss. Cult. Meth. 14, 31-36.; Cell. Mol. Biol. Res. 41(4), 241-249.; J. Comp. Physiol. A. 181, 111-119.; Anat. Embryol. 197, 421-437.

more info or order :

Developmental Studies Hybridoma Bank product webpage