laminin, beta-2 | Developmental Studies Hybridoma Bank D7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

laminin, beta-2

catalog :

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

rat, dog, cow

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
CHEN Y, Kikkawa Y, Miner J. A missense LAMB2 mutation causes congenital nephrotic syndrome by impairing laminin secretion. J Am Soc Nephrol. 2011;22:849-58 pubmed publisher
Underwood P, Bean P, Whitelock J. Inhibition of endothelial cell adhesion and proliferation by extracellular matrix from vascular smooth muscle cells: role of type V collagen. Atherosclerosis. 1998;141:141-52 pubmed
Iglesias M, Soler R, Hunter D, Ribera J, Esquerda J, Comella J. S-laminin and N-acetylgalactosamine located at the synaptic basal lamina of skeletal muscle are involved in synaptic recognition by growing neurites. J Neurocytol. 1995;24:903-15 pubmed
Walker Caprioglio H, Hunter D, McGuire P, Little S, McGuffee L. Composition in situ and in vitro of vascular smooth muscle laminin in the rat. Cell Tissue Res. 1995;281:187-96 pubmed
Martin P, Ettinger A, Sanes J. A synaptic localization domain in the synaptic cleft protein laminin beta 2 (s-laminin). Science. 1995;269:413-6 pubmed
Sanes J, Chiu A. The basal lamina of the neuromuscular junction. Cold Spring Harb Symp Quant Biol. 1983;48 Pt 2:667-78 pubmed
Hunter D, Shah V, Merlie J, Sanes J. A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction. Nature. 1989;338:229-34 pubmed
Sanes J, Engvall E, Butkowski R, Hunter D. Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol. 1990;111:1685-99 pubmed
Green T, Hunter D, Chan W, Merlie J, Sanes J. Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells. J Biol Chem. 1992;267:2014-22 pubmed
Chiu A, Ugozolli M, Meiri K, Ko J. Purification and lectin-binding properties of s-laminin, a synaptic isoform of the laminin B1 chain. J Neurochem. 1992;59:10-7 pubmed
Hunter D, Llinas R, Ard M, Merlie J, Sanes J. Expression of s-laminin and laminin in the developing rat central nervous system. J Comp Neurol. 1992;323:238-51 pubmed

product information

Internal ID :

2030

Name :

Depositor Name :

Sanes, J.R.

Depositor Institution :

Harvard University

Date Deposited :

7/22/92

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

laminin, beta-2

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Canine,Guinea Pig,Rabbit,Rat

Species Tested (legacy) :

rat, rabbit, guinea pig, dog

Initial Publication Pubmed ID :

6586383

Collections :

Cell adhesion,Cell signaling,Extracellular matrix proteins,Neurodevelopment

Search Keywords :

Sanes, J.R., laminin, beta-2, Bovine, MIgG1, rat/rabbit/guinea pig/canine , LAMB2, LAMS, AB_528330, monoclonal, Cell adhesion/Cell signaling/Extracellular matrix proteins/Neurodevelopment, ELISA/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 192 kDa; Apparent: 190 kDa (reducing conditions)

Gene :

LAMB2

Alternate Gene Name(s) :

LAMS

Uniprot ID :

E1BDK6

Antibody Registry ID :

AB_528330

Immunogen :

Adult bovine anterior lens capsule

Immunogen Sequence :

full length protein

Clonality :

Monoclonal

Myeloma Strain :

fox NY

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal, domain I region

Epitope Map PubMed ID :

2211832 7618109

Recommended Applications :

ELISA,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

2922051 2211832 1401258 7618109 7621523 8719818 21511833

Western Blot Pubmed IDs :

7618109 7621523 1613491

Immunoprecipitation Pubmed IDs :

1730732

ELISA Pubmed IDs :

9863547

Additional Information :

The former name of laminin beta-2 was S-laminin. D7 doesn't cross-react with mouse antigen. D7 doesn't appear to affect laminin beta-2 function. C4, D5 and D7 recognize different epitopes.

DSHB Growth Medium :

Iscove's

References (legacy) :

Nature 338, 229-233.; J. Cell Biol. 111, 1685-1699.; J. Biol. Chem. 267, 2014-2022.

more info or order :

Developmental Studies Hybridoma Bank product webpage