product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Desmin, intermediate filament
catalog :
D3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
D3
reactivity :
mouse, rat, chicken
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 15
Reference |
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Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, et al. High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo. Am J Physiol Cell Physiol. 2010;298:C465-76 pubmed publisher
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Kouloumenta A, Mavroidis M, Capetanaki Y. Proper perinuclear localization of the TRIM-like protein myospryn requires its binding partner desmin. J Biol Chem. 2007;282:35211-21 pubmed
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Mousavi K, Jasmin B. BDNF is expressed in skeletal muscle satellite cells and inhibits myogenic differentiation. J Neurosci. 2006;26:5739-49 pubmed
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McElhinny A, Perry C, Witt C, Labeit S, Gregorio C. Muscle-specific RING finger-2 (MURF-2) is important for microtubule, intermediate filament and sarcomeric M-line maintenance in striated muscle development. J Cell Sci. 2004;117:3175-88 pubmed
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Yablonka Reuveni Z, Rudnicki M, Rivera A, Primig M, Anderson J, Natanson P. The transition from proliferation to differentiation is delayed in satellite cells from mice lacking MyoD. Dev Biol. 1999;210:440-55 pubmed
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Graves D, Huiatt T, Zhou H, Huang H, Sernett S, Robson R, et al. Regulatory role of arginine-specific mono(ADP-ribosyl)transferase in muscle cells. Adv Exp Med Biol. 1997;419:305-13 pubmed
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Press R, Reddy E, Ewert D. Overexpression of C-terminally but not N-terminally truncated Myb induces fibrosarcomas: a novel nonhematopoietic target cell for the myb oncogene. Mol Cell Biol. 1994;14:2278-90 pubmed
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Danto S, Fischman D. Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin. J Cell Biol. 1984;98:2179-91 pubmed
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Fischman D, Danto S. Monoclonal antibodies to desmin: evidence for stage-dependent intermediate filament immunoreactivity during cardiac and skeletal muscle development. Ann N Y Acad Sci. 1985;455:167-84 pubmed
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Ralphs J, Dhoot G, Tickle C. Differentiation of myogenic cells in micromass cultures of cells from chick facial primordia. Dev Biol. 1989;131:189-96 pubmed
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Yablonka Reuveni Z, Nameroff M. Temporal differences in desmin expression between myoblasts from embryonic and adult chicken skeletal muscle. Differentiation. 1990;45:21-8 pubmed
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Baldwin H, Jensen K, Solursh M. Myogenic cytodifferentiation of the precardiac mesoderm in the rat. Differentiation. 1991;47:163-72 pubmed
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product information
Internal ID :
3076
Name :
D3
Depositor Name :
Fischman, D.A.
Depositor Institution :
Cornell University Medical College
Date Deposited :
4/22/86
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Desmin, intermediate filament
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgA, kappa light chain
Isotype for catalog (legacy) :
IgA, kappa light chain
Positive Tested Species Reactivity :
Chicken,Hamster,Mouse,Rat
Species Tested (legacy) :
positive in stage 26 chicken wing bud, day 19 rat limb bud and day 12 rat myotome
Initial Publication Pubmed ID :
6373792
Depositor Notes (Special Instructions) :
The D3 antibody is positive in stage 26 chick limb bud, day 19 rat limb bud and day 12 rat myotome. D3 also stained primary cultures of embryonic cardiac myocytes and H.H. stage 17-18 embryonic chick hearts.
Collections :
Cardiac development,Cytoskeleton,Human,Muscular dystrophy,Neurodevelopment,Skeletal muscle
Search Keywords :
Donald A. Fischman, Desmin, intermediate filament, Chicken, MIgA, kappa light chain, Chicken/Rat/Hamster/Mouse, DES, , AB_528195, Monoclonal, Cytoskeletal elements/Cardiac development/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western blot/ELISA/Immunoprecipitation
Antigen Molecular Weight :
53.2 kDa
Gene :
DES
Uniprot ID :
P02542
Antibody Registry ID :
AB_528195
Immunogen :
Acetic acid solubilized demins from chicken gizzard
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
SP2
Epitope Mapped :
Yes
Epitope Location or Sequence :
N-terminal rod domain
Epitope Map PubMed ID :
3909880
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
No
Immunohistochemistry Pubmed IDs :
2642428 3909880 23079662 21865583 20007454 8139533
Immunofluorescence Pubmed IDs :
6373792 2642428 2292359 1959714 20209123 16723531 15199100
Western Blot Pubmed IDs :
6373792 3909880 10357902
Immunoprecipitation Pubmed IDs :
9193670 17872945
ELISA Pubmed IDs :
6373792 3909880
Additional Information :
RID:AB_528195
DSHB Growth Medium :
Iscove's
more info or order :
company information
Developmental Studies Hybridoma Bank
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