laminin, gamma-1 | Developmental Studies Hybridoma Bank D18 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

laminin, gamma-1

catalog :

D18

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D18

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:400; loading ...; fig 3c	Lo Nigro A, de Jaime Soguero A, Khoueiry R, Cho D, Ferlazzo G, Perini I, et al. PDGFR?+ Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors. Stem Cell Reports. 2017;8:318-333 pubmed publisher
	Seeger T, Hart M, Patarroyo M, Rolauffs B, Aicher W, Klein G. Mesenchymal Stromal Cells for Sphincter Regeneration: Role of Laminin Isoforms upon Myogenic Differentiation. PLoS ONE. 2015;10:e0137419 pubmed publisher
	Sokoloff A, Li H, Burkholder T. Limited expression of slow tonic myosin heavy chain in human cranial muscles. Muscle Nerve. 2007;36:183-9 pubmed
	Gilchrist C, Chen J, Richardson W, Loeser R, Setton L. Functional integrin subunits regulating cell-matrix interactions in the intervertebral disc. J Orthop Res. 2007;25:829-40 pubmed
	McMillan J, Akiyama M, Nakamura H, Shimizu H. Colocalization of multiple laminin isoforms predominantly beneath hemidesmosomes in the upper lamina densa of the epidermal basement membrane. J Histochem Cytochem. 2006;54:109-18 pubmed
	Dedkov E, Borisov A, Wernig A, Carlson B. Aging of skeletal muscle does not affect the response of satellite cells to denervation. J Histochem Cytochem. 2003;51:853-63 pubmed
	Dedkov E, Kostrominova T, Borisov A, Carlson B. MyoD and myogenin protein expression in skeletal muscles of senile rats. Cell Tissue Res. 2003;311:401-16 pubmed
	Dedkov E, Kostrominova T, Borisov A, Carlson B. Survival of Schwann cells in chronically denervated skeletal muscles. Acta Neuropathol. 2002;103:565-74 pubmed
	Kutlesa S, Siler U, Speiser A, Wessels J, Virtanen I, Rousselle P, et al. Developmentally regulated interactions of human thymocytes with different laminin isoforms. Immunology. 2002;105:407-18 pubmed
	Martin S, Levine A, Chen Z, Ughrin Y, Levine J. Deposition of the NG2 proteoglycan at nodes of Ranvier in the peripheral nervous system. J Neurosci. 2001;21:8119-28 pubmed
	Dedkov E, Kostrominova T, Borisov A, Carlson B. Reparative myogenesis in long-term denervated skeletal muscles of adult rats results in a reduction of the satellite cell population. Anat Rec. 2001;263:139-54 pubmed
	Church H, Aplin J. BeWo choriocarcinoma cells produce laminin 10. Biochem J. 1998;332 ( Pt 2):491-8 pubmed
	Raabe E, Yoshida K, Schwarting G. Differential laminin isoform expression in the developing rat olfactory system. Brain Res Dev Brain Res. 1997;101:187-96 pubmed
	Miner J, Sanes J. Collagen IV alpha 3, alpha 4, and alpha 5 chains in rodent basal laminae: sequence, distribution, association with laminins, and developmental switches. J Cell Biol. 1994;127:879-91 pubmed
	Sanes J, Engvall E, Butkowski R, Hunter D. Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol. 1990;111:1685-99 pubmed
	Green T, Hunter D, Chan W, Merlie J, Sanes J. Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells. J Biol Chem. 1992;267:2014-22 pubmed

product information

Internal ID :

2018

Name :

D18

Depositor Name :

Sanes, J.R.

Depositor Institution :

Harvard University

Date Deposited :

7/22/92

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

laminin, gamma-1

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a, kappa light chain

Positive Tested Species Reactivity :

Guinea Pig,Human,Rat

Species Tested (legacy) :

rat, guinea pig, human

Initial Publication Pubmed ID :

2211832

Collections :

Cell adhesion,Cell signaling,Extracellular matrix proteins,Human,Neurodevelopment

Search Keywords :

Sanes, J.R., laminin, gamma-1, Rat, MIgG2a, kappa light chain, rat/guinea pig/human, Lamc1, B2e, AB_2281095, monoclonal, Cell adhesion/Cell signaling/Extracellular matrix proteins/Neurodevelopment/Human, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 174 kDa; Apparent: 200 kDa

Gene :

Lamc1

Alternate Gene Name(s) :

B2e

Uniprot ID :

F1MAA7

Antibody Registry ID :

AB_2281095

Immunogen :

Adult rat glomerular basement membrane.

Immunogen Sequence :

full length protein

Clonality :

Monoclonal

Myeloma Strain :

fox NY

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

17486578

Immunofluorescence Pubmed IDs :

2211832 12012088 12810836 16174790 26406476 9601079 7962065 11985661 11588184 17318895 9263592

Western Blot Pubmed IDs :

2211832

Immunoprecipitation Pubmed IDs :

1730732

Pubmed IDs :

11360231 12658448

Additional Information :

The former name of laminin gamma-1 was laminin B2.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 111, 1685-1699.; J. Biol. Chem. 267, 2014-2022.; Anat. Rec. 263, 139-154.; Acta. Neuropathol. 103, 565-574.; J. Histochem. Cytochem. 51(7), 853-863.; Cell Tissue Res. 311, 401-416.; J. Histochem. Cytochem. 54(1), 109-118.; Muscle Nerve 36, 183-189.

more info or order :

Developmental Studies Hybridoma Bank product webpage