product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
TGFbeta3, functional, active domain
catalog :
D-b3(act)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
D-B3(act)
reactivity :
human, chicken
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 4
Reference
Delorme S, Lungu I, Vickaryous M. Scar-free wound healing and regeneration following tail loss in the leopard gecko, Eublepharis macularius. Anat Rec (Hoboken). 2012;295:1575-95 pubmed publisher
Nagchowdhuri P, Andrews K, Robart S, Capehart A. Versican knockdown reduces interzone area during early stages of chick synovial joint development. Anat Rec (Hoboken). 2012;295:397-409 pubmed publisher
Robb S, SANCHEZ ALVARADO A. Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy. Genesis. 2002;32:293-8 pubmed
Boyer A, Ayerinskas I, Vincent E, McKinney L, Weeks D, Runyan R. TGFbeta2 and TGFbeta3 have separate and sequential activities during epithelial-mesenchymal cell transformation in the embryonic heart. Dev Biol. 1999;208:530-45 pubmed
product information
Internal ID :
1945
Name :
D-B3(act)
Depositor Name :
Runyan, R.
Depositor Institution :
Department of Cell Biology and Anatomy, University of Arizona
Date Deposited :
2/18/99
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
TGFbeta3, functional, active domain
Antigen Species :
chicken
Host Species :
mouse
Isotype :
MIgM
Isotype for catalog (legacy) :
IgM
Positive Tested Species Reactivity :
Chicken,Lizard,Mammal,Planaria
Species Tested (legacy) :
chick, should recognize mammalian TGFß3 (not tested), planaria
Initial Publication Pubmed ID :
10191064
Depositor Notes (Special Instructions) :
Stains chick embryo heart.
Collections :
Cardiac development,Cell signaling
Search Keywords :
Runyan, R., TGFbeta3, functional, active domain, chicken, MIgM, chicken/planaria/mammal/lizard, TGFB3, TGF-beta3, AB_531801, monoclonal, Cell signaling/Cardiac development, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA/Function Blocking/FFPE
Antigen Molecular Weight :
Predicted: 45 kDa, 32 kDa (latent peptide), 13 kDa (TGFbate3 active peptide)
Gene :
TGFB3
Alternate Gene Name(s) :
TGF-beta3
Uniprot ID :
P16047
Antibody Registry ID :
AB_531801
Synonyms (Alternate Clone Names) :
D-B3
Immunogen :
Recombinant GST/TGFbeta3 fusion protein (one of three boosts with active domain of TGFbeta3)
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS-1
Epitope Mapped :
Yes
Epitope Location or Sequence :
TGFbeta3 N-terminus (ALDTNY)
Epitope Map PubMed ID :
10191064
Recommended Applications :
ELISA,FFPE,Function Blocking,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
22933425
Immunofluorescence Pubmed IDs :
10191064 11948917 22190409
Western Blot Pubmed IDs :
10191064
ELISA Pubmed IDs :
10191064
FFPE Pubmed IDs :
10191064
Function Blocking Pubmed IDs :
10191064
DSHB Growth Medium :
RPMI
References (legacy) :
Dev. Biol. 208(2), 530-545.; Genesis 32, 293-298.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US