Crumbs Drosophila protein (extracellular domain) | Developmental Studies Hybridoma Bank Cq4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Crumbs Drosophila protein (extracellular domain)

catalog :

Cq4

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Cq4

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:300; loading ...; tbl 3	Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
immunohistochemistry; fruit fly; 1:250	SHERRARD K, Fehon R. The transmembrane protein Crumbs displays complex dynamics during follicular morphogenesis and is regulated competitively by Moesin and aPKC. Development. 2015;142:1869-78 pubmed publisher
	Zhu Y, Li D, Wang Y, Pei C, Liu S, Zhang L, et al. Brahma regulates the Hippo pathway activity through forming complex with Yki-Sd and regulating the transcription of Crumbs. Cell Signal. 2015;27:606-13 pubmed publisher
	Ribeiro P, Holder M, Frith D, Snijders A, Tapon N. Crumbs promotes expanded recognition and degradation by the SCF(Slimb/β-TrCP) ubiquitin ligase. Proc Natl Acad Sci U S A. 2014;111:E1980-9 pubmed publisher
	Jeon M, Scott M, Zinn K. Interactions between Type III receptor tyrosine phosphatases and growth factor receptor tyrosine kinases regulate tracheal tube formation in Drosophila. Biol Open. 2012;1:548-58 pubmed publisher
	Sen A, Nagy Zsvér Vadas Z, Krahn M. Drosophila PATJ supports adherens junction stability by modulating Myosin light chain activity. J Cell Biol. 2012;199:685-98 pubmed publisher
	Zhou B, Wu Y, Lin X. Retromer regulates apical-basal polarity through recycling Crumbs. Dev Biol. 2011;360:87-95 pubmed publisher
	Galy A, Schenck A, Sahin H, Qurashi A, Sahel J, Diebold C, et al. CYFIP dependent actin remodeling controls specific aspects of Drosophila eye morphogenesis. Dev Biol. 2011;359:37-46 pubmed publisher
	Morais de Sá E, Mirouse V, St Johnston D. aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells. Cell. 2010;141:509-23 pubmed publisher
	Maurel Zaffran C, Pradel J, Graba Y. Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis. Dev Biol. 2010;343:18-27 pubmed publisher
	Chanana B, Steigemann P, Jackle H, Vorbrüggen G. Reception of Slit requires only the chondroitin-sulphate-modified extracellular domain of Syndecan at the target cell surface. Proc Natl Acad Sci U S A. 2009;106:11984-8 pubmed publisher
	Bulgakova N, Kempkens O, Knust E. Multiple domains of Stardust differentially mediate localisation of the Crumbs-Stardust complex during photoreceptor development in Drosophila. J Cell Sci. 2008;121:2018-26 pubmed publisher
	Li Z, Wang L, Hays T, Cai Y. Dynein-mediated apical localization of crumbs transcripts is required for Crumbs activity in epithelial polarity. J Cell Biol. 2008;180:31-8 pubmed publisher
	Kempkens O, Médina E, Fernandez Ballester G, Ozüyaman S, Le Bivic A, Serrano L, et al. Computer modelling in combination with in vitro studies reveals similar binding affinities of Drosophila Crumbs for the PDZ domains of Stardust and DmPar-6. Eur J Cell Biol. 2006;85:753-67 pubmed
	Sotillos S, Diaz Meco M, Caminero E, Moscat J, Campuzano S. DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila. J Cell Biol. 2004;166:549-57 pubmed
	Deng W, Schneider M, Frock R, Castillejo Lopez C, Gaman E, Baumgartner S, et al. Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila. Development. 2003;130:173-84 pubmed
	Bachmann A, Schneider M, Theilenberg E, Grawe F, Knust E. Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity. Nature. 2001;414:638-43 pubmed
	Baumann O. Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia. Exp Cell Res. 2001;270:176-87 pubmed
	Klebes A, Knust E. A conserved motif in Crumbs is required for E-cadherin localisation and zonula adherens formation in Drosophila. Curr Biol. 2000;10:76-85 pubmed
	Tepass U. Crumbs, a component of the apical membrane, is required for zonula adherens formation in primary epithelia of Drosophila. Dev Biol. 1996;177:217-25 pubmed
	Tepass U, Gruszynski deFeo E, Haag T, Omatyar L, Torok T, Hartenstein V. shotgun encodes Drosophila E-cadherin and is preferentially required during cell rearrangement in the neurectoderm and other morphogenetically active epithelia. Genes Dev. 1996;10:672-85 pubmed
	Tepass U, Knust E. Crumbs and stardust act in a genetic pathway that controls the organization of epithelia in Drosophila melanogaster. Dev Biol. 1993;159:311-26 pubmed
	Hartenstein A, Rugendorff A, Tepass U, Hartenstein V. The function of the neurogenic genes during epithelial development in the Drosophila embryo. Development. 1992;116:1203-20 pubmed

product information

Internal ID :

2899

Name :

Cq4

Depositor Name :

Knust, E.

Depositor Institution :

Max Planck Institute of Molecular Cell Biology and Genetics

Date Deposited :

9/27/00

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Crumbs Drosophila protein (extracellular domain)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila melanogaster,Drosophila virilis

Species Tested (legacy) :

Drosophila, not mouse or C. elegans

Initial Publication Pubmed ID :

8365569

Depositor Notes (Special Instructions) :

crossreacts with Drosophila virilis, not with mouse or C. elegans. Crumbs protein is expressed in all epithelia of ectodermal origin in the Drosophila embryo from gastrulation onwards and in all imaginal discs and the follicle epithelium. It is restricted to a region apical to the zonula adherens. Good apical marker for epithelial cells.

Collections :

Cell markers,Cell signaling,Drosophila antigens

Search Keywords :

Knust, E., Crumbs Drosophila protein (extracellular domain), Drosophila, MIgG1, kappa light chain, Drosophila melanogaster/Drosophila virilis, crb, CG6383, AB_528181, monoclonal, Drosophila antigens/Cell signaling/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 234 kDa

Gene :

crb

Alternate Gene Name(s) :

CG6383

Uniprot ID :

P10040

Antibody Registry ID :

AB_528181

Immunogen :

Recombinant fusion protein between beta-galactosidase and aa 737-1703 of Drosophila Crumbs protein.

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 737-1703

Epitope Map PubMed ID :

8365569

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

8365569 1295737 11740560 12441301 8598295 31405903

Immunofluorescence Pubmed IDs :

11640882 20434988 20403348 21884694 10662667 18195099 25496831 16697075 11740560 18495840 19574454 12441301 24778256

Western Blot Pubmed IDs :

18195099 25496831 16697075 18495840 21958744 24778256

Pubmed IDs :

8660889

Additional Information :

For experiments involving live cells and tissue, Cq4 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 61, 787-799.; Roux’s Arch. Dev. Biol. 199, 189-206.; Dev. Biol. 159, 311-326.; Exp. Cell Res. 270, 176-187.; Dev. Biol. 343, 18-27.; Cell 141, 509-523.; Dev. Biol. 359, 37-46.

more info or order :

Developmental Studies Hybridoma Bank product webpage