choline acetyltransferase | Developmental Studies Hybridoma Bank ChAT4B1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

choline acetyltransferase

catalog :

ChAT4B1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ChAT4B1

reactivity :

fruit fly

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; loading ...; fig 3a	Schlegel P, Texada M, Miroschnikow A, Schoofs A, Hückesfeld S, Peters M, et al. Synaptic transmission parallels neuromodulation in a central food-intake circuit. elife. 2016;5: pubmed publisher
immunohistochemistry; fruit fly; 1:500; loading ...; fig 4c	Sizemore T, Dacks A. Serotonergic Modulation Differentially Targets Distinct Network Elements within the Antennal Lobe of Drosophila melanogaster. Sci Rep. 2016;6:37119 pubmed publisher
immunohistochemistry; fruit fly; 1:500	Berck M, Khandelwal A, Claus L, Hernandez Nunez L, Si G, Tabone C, et al. The wiring diagram of a glomerular olfactory system. elife. 2016;5: pubmed publisher
immunocytochemistry; fruit fly; 1:100	Rohwedder A, Selcho M, Chassot B, Thum A. Neuropeptide F neurons modulate sugar reward during associative olfactory learning of Drosophila larvae. J Comp Neurol. 2015;523:2637-64 pubmed publisher
immunohistochemistry; fruit fly; 1:100	Selcho M, Pauls D, Huser A, Stocker R, Thum A. Characterization of the octopaminergic and tyraminergic neurons in the central brain of Drosophila larvae. J Comp Neurol. 2014;522:3485-500 pubmed publisher
immunohistochemistry; fruit fly; 1:100	Apostolopoulou A, Mazija L, Wüst A, Thum A. The neuronal and molecular basis of quinine-dependent bitter taste signaling in Drosophila larvae. Front Behav Neurosci. 2014;8:6 pubmed publisher
	Lacin H, Chen H, Long X, Singer R, Lee T, Truman J. Neurotransmitter identity is acquired in a lineage-restricted manner in the Drosophila CNS. elife. 2019;8: pubmed publisher
	Augustin H, McGourty K, Allen M, Madem S, Adcott J, Kerr F, et al. Reduced insulin signaling maintains electrical transmission in a neural circuit in aging flies. PLoS Biol. 2017;15:e2001655 pubmed publisher
	Eichler K, Li F, Litwin Kumar A, Park Y, Andrade I, Schneider Mizell C, et al. The complete connectome of a learning and memory centre in an insect brain. Nature. 2017;548:175-182 pubmed publisher
	Pankova K, Borst A. RNA-Seq Transcriptome Analysis of Direction-Selective T4/T5 Neurons in Drosophila. PLoS ONE. 2016;11:e0163986 pubmed publisher
	Kidd S, Lieber T. Mechanism of Notch Pathway Activation and Its Role in the Regulation of Olfactory Plasticity in Drosophila melanogaster. PLoS ONE. 2016;11:e0151279 pubmed publisher
	Ichinose T, Aso Y, Yamagata N, Abe A, Rubin G, Tanimoto H. Reward signal in a recurrent circuit drives appetitive long-term memory formation. elife. 2015;4:e10719 pubmed publisher
	Fusca D, Schachtner J, Kloppenburg P. Colocalization of allatotropin and tachykinin-related peptides with classical transmitters in physiologically distinct subtypes of olfactory local interneurons in the cockroach (Periplaneta americana). J Comp Neurol. 2015;523:1569-86 pubmed publisher
	Aso Y, Hattori D, Yu Y, Johnston R, Iyer N, Ngo T, et al. The neuronal architecture of the mushroom body provides a logic for associative learning. elife. 2014;3:e04577 pubmed publisher
	Iacobucci G, Rahman N, Valtueña A, Nayak T, Gunawardena S. Spatial and temporal characteristics of normal and perturbed vesicle transport. PLoS ONE. 2014;9:e97237 pubmed publisher
	Haddadi M, Jahromi S, Sagar B, Patil R, Shivanandappa T, Ramesh S. Brain aging, memory impairment and oxidative stress: a study in Drosophila melanogaster. Behav Brain Res. 2014;259:60-9 pubmed publisher
	Karsai G, Pollak E, Wacker M, Vömel M, Selcho M, Berta G, et al. Diverse in- and output polarities and high complexity of local synaptic and non-synaptic signaling within a chemically defined class of peptidergic Drosophila neurons. Front Neural Circuits. 2013;7:127 pubmed publisher
	Levy P, Larsen C. Odd-skipped labels a group of distinct neurons associated with the mushroom body and optic lobe in the adult Drosophila brain. J Comp Neurol. 2013;521:3716-40 pubmed publisher
	Das A, Gupta T, Davla S, Prieto Godino L, Diegelmann S, Reddy O, et al. Neuroblast lineage-specific origin of the neurons of the Drosophila larval olfactory system. Dev Biol. 2013;373:322-37 pubmed publisher
	Selcho M, Pauls D, El Jundi B, Stocker R, Thum A. The role of octopamine and tyramine in Drosophila larval locomotion. J Comp Neurol. 2012;520:3764-85 pubmed publisher
	Butcher N, Friedrich A, Lu Z, Tanimoto H, Meinertzhagen I. Different classes of input and output neurons reveal new features in microglomeruli of the adult Drosophila mushroom body calyx. J Comp Neurol. 2012;520:2185-201 pubmed publisher
	Thum A, Leisibach B, Gendre N, Selcho M, Stocker R. Diversity, variability, and suboesophageal connectivity of antennal lobe neurons in D. melanogaster larvae. J Comp Neurol. 2011;519:3415-32 pubmed publisher
	Varija Raghu S, Reiff D, Borst A. Neurons with cholinergic phenotype in the visual system of Drosophila. J Comp Neurol. 2011;519:162-76 pubmed publisher
	LaJeunesse D, Johnson B, Presnell J, Catignas K, Zapotoczny G. Peristalsis in the junction region of the Drosophila larval midgut is modulated by DH31 expressing enteroendocrine cells. BMC Physiol. 2010;10:14 pubmed publisher
	Seki Y, Rybak J, Wicher D, Sachse S, Hansson B. Physiological and morphological characterization of local interneurons in the Drosophila antennal lobe. J Neurophysiol. 2010;104:1007-19 pubmed publisher
	Leiss F, Groh C, Butcher N, Meinertzhagen I, Tavosanis G. Synaptic organization in the adult Drosophila mushroom body calyx. J Comp Neurol. 2009;517:808-24 pubmed publisher
	Kolodziejczyk A, Sun X, Meinertzhagen I, Nassel D. Glutamate, GABA and acetylcholine signaling components in the lamina of the Drosophila visual system. PLoS ONE. 2008;3:e2110 pubmed publisher
	Vömel M, Wegener C. Neurotransmitter-induced changes in the intracellular calcium concentration suggest a differential central modulation of CCAP neuron subsets in Drosophila. Dev Neurobiol. 2007;67:792-808 pubmed
	Bader R, Colomb J, Pankratz B, Schröck A, Stocker R, Pankratz M. Genetic dissection of neural circuit anatomy underlying feeding behavior in Drosophila: distinct classes of hugin-expressing neurons. J Comp Neurol. 2007;502:848-56 pubmed
	Colomb J, Grillenzoni N, Ramaekers A, Stocker R. Architecture of the primary taste center of Drosophila melanogaster larvae. J Comp Neurol. 2007;502:834-47 pubmed
	Shang Y, Claridge Chang A, Sjulson L, Pypaert M, Miesenbock G. Excitatory local circuits and their implications for olfactory processing in the fly antennal lobe. Cell. 2007;128:601-12 pubmed
	Clark J, Meisner S, Torkkeli P. Immunocytochemical localization of choline acetyltransferase and muscarinic ACh receptors in the antenna during development of the sphinx moth Manduca sexta. Cell Tissue Res. 2005;320:163-73 pubmed
	Torkkeli P, Widmer A, Meisner S. Expression of muscarinic acetylcholine receptors and choline acetyltransferase enzyme in cultured antennal sensory neurons and non-neural cells of the developing moth Manduca sexta. J Neurobiol. 2005;62:316-29 pubmed
	Python F, Stocker R. Immunoreactivity against choline acetyltransferase, gamma-aminobutyric acid, histamine, octopamine, and serotonin in the larval chemosensory system of Dosophila melanogaster. J Comp Neurol. 2002;453:157-67 pubmed
	Lee M, Salvaterra P. Abnormal chemosensory jump 6 is a positive transcriptional regulator of the cholinergic gene locus in Drosophila olfactory neurons. J Neurosci. 2002;22:5291-9 pubmed
	Yasuyama K, Meinertzhagen I, Schürmann F. Synaptic organization of the mushroom body calyx in Drosophila melanogaster. J Comp Neurol. 2002;445:211-26 pubmed
	Bowman A, Patel King R, Benashski S, McCaffery J, Goldstein L, King S. Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis. J Cell Biol. 1999;146:165-80 pubmed
	Takagawa K, Salvaterra P. Analysis of choline acetyltransferase protein in temperature sensitive mutant flies using newly generated monoclonal antibody. Neurosci Res. 1996;24:237-43 pubmed
	Yasuyama K, Kitamoto T, Salvaterra P. Localization of choline acetyltransferase-expressing neurons in the larval visual system of Drosophila melanogaster. Cell Tissue Res. 1995;282:193-202 pubmed

product information

Internal ID :

1990

Name :

ChAT4B1

Depositor Name :

Salvaterra, P.M.

Depositor Institution :

Beckman Research Institute, City of Hope

Date Deposited :

9/6/02

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

choline acetyltransferase

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila,Manduca sexta

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8815444

Collections :

Cell signaling,Drosophila antigens,Enzymes,Neurodevelopment

Search Keywords :

Salvaterra, P.M., choline acetyltransferase, Drosophila, MIgG1, Drosophila/Manduca sexta, cha, CG12345, ChAT, AB_528122, Monoclonal, Cell signaling/Drosophila antigens/Neurodevelopment/Enzymes, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA/FFPE

Antigen Molecular Weight :

81 kDa; cleaved into three chains of 13, 54, and 67 kDa

Gene :

cha

Alternate Gene Name(s) :

CG12345, ChAT

Uniprot ID :

P07668

Antibody Registry ID :

AB_528122

Synonyms (Alternate Clone Names) :

4B1

Immunogen :

Recombinant fusion protein

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0

Epitope Mapped :

Recommended Applications :

ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8565051 15719247 11920702 27684367

Immunofluorescence Pubmed IDs :

17443825 20505124 10402468 17289577 21120933 23149077 15514997 18464935 22237598 24183945 12373781 24752702 25678036 26234537 31391301

Western Blot Pubmed IDs :

8815444 15514997 12097480 24752702

Immunoprecipitation Pubmed IDs :

8815444

ELISA Pubmed IDs :

8815444

FFPE Pubmed IDs :

11920702

Additional Information :

Choline O-acetyltransferase catalyzes the reversible synthesis of acetylcholine from acetyl CoA and choline at cholinergic synapses.

DSHB Growth Medium :

RPMI

References (legacy) :

Neurosci. Res. 24, 237-243.; Dev. Neurobiol. 67(6), 792-808.; J. Neurophysiol. 104, 1007-1019.

more info or order :

Developmental Studies Hybridoma Bank product webpage