SAX-7 | Developmental Studies Hybridoma Bank CeSAX7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

SAX-7

catalog :

CeSAX7

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CeSAX7

reactivity :

Caenorhabditis elegans

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 3

Reference
Klompstra D, Anderson D, Yeh J, Zilberman Y, Nance J. An instructive role for C. elegans E-cadherin in translating cell contact cues into cortical polarity. Nat Cell Biol. 2015;17:726-35 pubmed publisher
Cheng L, Li B, Wang Z. Level-Set Minimization of Potential Controlled Hadwiger Valuations for Molecular Solvation. J Comput Phys. 2010;229:8497-8510 pubmed
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher

product information

Internal ID :

6523

Name :

CeSAX7

Depositor Name :

Nonet, M.L. / Hadwiger, G. / Dour, S.

Depositor Institution :

Washington University Medical School

Date Deposited :

2/16/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

SAX-7

Antigen Species :

C. elegans

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

C. elegans

Species Tested (legacy) :

C. elegans

Initial Publication Pubmed ID :

20405020

Depositor Notes (Special Instructions) :

Works on C. elegans whole mounts. Stains the plasma membrane at cell-cell contacts. Works poorly in detecting SAX-7 on immunoblots. By western blot, CeSAX7 identifies a band of 55 kDa which may represent a post-translational cleaved product [PMID: 20405020].

Collections :

C. elegans,Cell adhesion,Neurodevelopment

Search Keywords :

Nonet, M.L. / Hadwiger, G. / Dour, S., SAX-7, C. elegans, MIgG1, C. elegans, sax-7, , AB_10567266, monoclonal, C. elegans/cell adhesion/Neurodevelopment, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 124 (shortform) and 145 (longform) kDa; Apparent: multiple bands between 45 and 200 kDa

Gene :

sax-7

Uniprot ID :

Q53U86 Q53U87

Antibody Registry ID :

AB_10567266

Synonyms (Alternate Clone Names) :

SAX7, 11.2.1

Immunogen :

Recombinant His6-tagged SAX-7 (C-terminal cytoplasmic domain 94 aa) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal cytoplasmic domain 94 aa

Epitope Map PubMed ID :

20405020

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

poor

Immunofluorescence Pubmed IDs :

20405020 25938815

Western Blot Pubmed IDs :

20405020

Additional Information :

SAX-7 is a homolog of the cell adhesion molecule L1/CAM which localizes most prominently to sites of cell-cell contact in the nervous system.

DSHB Growth Medium :

Iscove's

References (legacy) :

Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. (2010) A monoclonal antibody toolkit for C. elegans. PLoS One. 5(4):e10161-10115.

more info or order :

Developmental Studies Hybridoma Bank product webpage