product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
ATPase, Ca(+2) slow twitch/cardiac SR (avian), SERCA2
catalog :
CaS/C1 or 3H2
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CaS/C1 or 3H2
reactivity :
chicken
application :
western blot, immunocytochemistry
citations: 15
Reference
Imamura M, Nakamura A, Mannen H, Takeda S. Characterization of WWP1 protein expression in skeletal muscle of muscular dystrophy chickens. J Biochem. 2016;159:171-9 pubmed publisher
Yamazaki H, Chan J, Ikura M, Michikawa T, Mikoshiba K. Tyr-167/Trp-168 in type 1/3 inositol 1,4,5-trisphosphate receptor mediates functional coupling between ligand binding and channel opening. J Biol Chem. 2010;285:36081-91 pubmed publisher
Seth M, Sumbilla C, Mullen S, Lewis D, Klein M, Hussain A, et al. Sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) gene silencing and remodeling of the Ca2+ signaling mechanism in cardiac myocytes. Proc Natl Acad Sci U S A. 2004;101:16683-8 pubmed
Ma H, Sumbilla C, Farrance I, Klein M, Inesi G. Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes. Am J Physiol Cell Physiol. 2004;286:C556-64 pubmed
Sumbilla C, Ma H, Seth M, Inesi G. Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes. Arch Biochem Biophys. 2003;415:178-83 pubmed
Cavagna M, O Donnell J, Sumbilla C, Inesi G, Klein M. Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes. J Physiol. 2000;528 Pt 1:53-63 pubmed
Inesi G, Lewis D, Sumbilla C, Nandi A, Strock C, Huff K, et al. Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes. Am J Physiol. 1998;274:C645-53 pubmed publisher
Sumbilla C, Lu L, Lewis D, Inesi G, Ishii T, Takeyasu K, et al. Ca(2+)-dependent and thapsigargin-inhibited phosphorylation of Na+,K(+)-ATPase catalytic domain following chimeric recombination with Ca(2+)-ATPase. J Biol Chem. 1993;268:21185-92 pubmed
Airey J, Deerinck T, Ellisman M, Houenou L, Ivanenko A, Kenyon J, et al. Crooked neck dwarf (cn) mutant chicken skeletal muscle cells in low density primary cultures fail to express normal alpha ryanodine receptor and exhibit a partial mutant phenotype. Dev Dyn. 1993;197:189-202 pubmed
Airey J, Baring M, Beck C, Chelliah Y, Deerinck T, Ellisman M, et al. Failure to make normal alpha ryanodine receptor is an early event associated with the crooked neck dwarf (cn) mutation in chicken. Dev Dyn. 1993;197:169-88 pubmed
Dumonteil E, Barre H, Meissner G. Expression of sarcoplasmic reticulum Ca2+ transport proteins in cold-acclimating ducklings. Am J Physiol. 1995;269:C955-60 pubmed
Kaprielian Z, Fambrough D. Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle. Dev Biol. 1987;124:490-503 pubmed
Karin N, Kaprielian Z, Fambrough D. Expression of avian Ca2+-ATPase in cultured mouse myogenic cells. Mol Cell Biol. 1989;9:1978-86 pubmed
Campbell A, Kessler P, Sagara Y, Inesi G, Fambrough D. Nucleotide sequences of avian cardiac and brain SR/ER Ca(2+)-ATPases and functional comparisons with fast twitch Ca(2+)-ATPase. Calcium affinities and inhibitor effects. J Biol Chem. 1991;266:16050-5 pubmed
Campbell A, Kessler P, Fambrough D. The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane. J Biol Chem. 1992;267:9321-5 pubmed
product information
Internal ID :
2743
Name :
CaS/C1 or 3H2
Depositor Name :
Fambrough, D.M.
Depositor Institution :
Johns Hopkins School of Medicine
Depositor Website :
http://www.searlescholars.net/person/480
Date Deposited :
10/10/90
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
ATPase, Ca(+2) slow twitch/cardiac SR (avian), SERCA2
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Chicken,Duck
Species Tested (legacy) :
chicken specific
Initial Publication Pubmed ID :
2960578
Depositor Notes (Special Instructions) :
Immunoblotting: very good. Immunohistochemistry: good on cryosections and formaldehyde fixed tissues. The epitope is destroyed with methanol and glutaraldehyde.
Collections :
Cardiac development,Enzymes,Neurodevelopment,Skeletal muscle
Search Keywords :
Fambrough, D. M., ATPase, Ca(+2) slow twitch/cardiac SR (avian), SERCA2, Chicken, MIgG1, kappa light chain, Chicken/Duck, ATP2A2, SERCA2, AB_531764, monoclonal, Cardiac development/Enzymes/Neurodevelopment/Skeletal muscle, Immunofluorescence/Western Blot
Antigen Molecular Weight :
115 kDa
Gene :
ATP2A2
Alternate Gene Name(s) :
SERCA2
Uniprot ID :
Q03669
Antibody Registry ID :
AB_531764
Synonyms (Alternate Clone Names) :
Cas-3H2; 3H2
Immunogen :
Sarcoplasmic reticulum membrane vesicles preparation from adult chicken hearts.
Immunogen Sequence :
Total protein
Clonality :
Monoclonal
Myeloma Strain :
SP2/0 Ag8
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Western Blot
Immunoblotting (legacy) :
5 ug/ml very good
Immunofluorescence Pubmed IDs :
2960578 26314333 15546997 14592812 11018105 1831452
Western Blot Pubmed IDs :
2960578 9530095 8407954 11018105 12831840 1533629 7485465 1831452
Pubmed IDs :
8219359 20813840 8219360 2526293
DSHB Growth Medium :
Iscove's
References (legacy) :
Dev. Biol. 124, 490-503.; Dev. Dyn. 197, 169-188.; J. Biol. Chem. 285(46), 36081-36091.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US