ATPase, Ca(+2) fast twitch SR / SERCA1 | Developmental Studies Hybridoma Bank CaF2-5D2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

ATPase, Ca(+2) fast twitch SR / SERCA1

catalog :

CaF2-5D2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CaF2-5D2

reactivity :

human, mouse, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 6	Horvath D, Murphy R, Mollica J, Hayes A, Goodman C. The effect of taurine and Î²-alanine supplementation on taurine transporter protein and fatigue resistance in skeletal muscle from mdx mice. Amino Acids. 2016;48:2635-2645 pubmed
western blot; human; 1:1000; fig 1a	Lamboley C, Murphy R, McKenna M, Lamb G. Sarcoplasmic reticulum Ca2+ uptake and leak properties, and SERCA isoform expression, in type I and type II fibres of human skeletal muscle. J Physiol. 2014;592:1381-95 pubmed publisher
	Demonbreun A, Rossi A, Alvarez M, Swanson K, Deveaux H, Earley J, et al. Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity. Am J Pathol. 2014;184:248-59 pubmed publisher
	Murphy R, Vissing K, Latchman H, Lamboley C, McKenna M, Overgaard K, et al. Activation of skeletal muscle calpain-3 by eccentric exercise in humans does not result in its translocation to the nucleus or cytosol. J Appl Physiol (1985). 2011;111:1448-58 pubmed publisher
	Murphy R. Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1. J Appl Physiol (1985). 2011;110:820-5 pubmed publisher
	Summerfield N, Peters M, Hercock C, Mobasheri A, Young I. Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium. J Vet Cardiol. 2010;12:17-23 pubmed publisher
	Goodman C, Horvath D, Stathis C, Mori T, Croft K, Murphy R, et al. Taurine supplementation increases skeletal muscle force production and protects muscle function during and after high-frequency in vitro stimulation. J Appl Physiol (1985). 2009;107:144-54 pubmed publisher
	Murphy R, Lamb G. Endogenous calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is not dependent on stretch. J Biol Chem. 2009;284:7811-9 pubmed publisher
	Murphy R, Larkins N, Mollica J, Beard N, Lamb G. Calsequestrin content and SERCA determine normal and maximal Ca2+ storage levels in sarcoplasmic reticulum of fast- and slow-twitch fibres of rat. J Physiol. 2009;587:443-60 pubmed publisher
	Ramnanan C, Storey K. The regulation of thapsigargin-sensitive sarcoendoplasmic reticulum Ca(2+)-ATPase activity in estivation. J Comp Physiol B. 2008;178:33-45 pubmed
	de Groof A, Oerlemans F, Jost C, Wieringa B. Changes in glycolytic network and mitochondrial design in creatine kinase-deficient muscles. Muscle Nerve. 2001;24:1188-96 pubmed
	Takekura H, Franzini Armstrong C. Correct targeting of dihydropyridine receptors and triadin in dyspedic mouse skeletal muscle in vivo. Dev Dyn. 1999;214:372-80 pubmed
	Ishii T, Hata F, Lemas M, Fambrough D, Takeyasu K. Carboxy-terminal regions of the sarcoplasmic/endoplasmic reticulum Ca(2+)- and the Na+/K(+)-ATPases control their K+ sensitivity. Biochemistry. 1997;36:442-51 pubmed
	Ishii T, Takeyasu K. The amino-terminal 200 amino acids of the plasma membrane Na+,K+-ATPase alpha subunit confer ouabain sensitivity on the sarcoplasmic reticulum Ca(2+)-ATPase. Proc Natl Acad Sci U S A. 1993;90:8881-5 pubmed
	Airey J, Deerinck T, Ellisman M, Houenou L, Ivanenko A, Kenyon J, et al. Crooked neck dwarf (cn) mutant chicken skeletal muscle cells in low density primary cultures fail to express normal alpha ryanodine receptor and exhibit a partial mutant phenotype. Dev Dyn. 1993;197:189-202 pubmed
	Airey J, Baring M, Beck C, Chelliah Y, Deerinck T, Ellisman M, et al. Failure to make normal alpha ryanodine receptor is an early event associated with the crooked neck dwarf (cn) mutation in chicken. Dev Dyn. 1993;197:169-88 pubmed
	Benders A, Veerkamp J, Oosterhof A, Jongen P, Bindels R, Smit L, et al. Ca2+ homeostasis in Brody's disease. A study in skeletal muscle and cultured muscle cells and the effects of dantrolene an verapamil. J Clin Invest. 1994;94:741-8 pubmed
	Kaprielian Z, Fambrough D. Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle. Dev Biol. 1987;124:490-503 pubmed
	Karin N, Kaprielian Z, Fambrough D. Expression of avian Ca2+-ATPase in cultured mouse myogenic cells. Mol Cell Biol. 1989;9:1978-86 pubmed

product information

Internal ID :

2731

Name :

CaF2-5D2

Depositor Name :

Fambrough, D.M.

Depositor Institution :

Johns Hopkins School of Medicine

Depositor Website :

http://www.searlescholars.net/person/475

Date Deposited :

10/10/90

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

ATPase, Ca(+2) fast twitch SR / SERCA1

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Human,Mouse,Rat,Vertebrates

Species Tested (legacy) :

all vertebrates tested to date

Initial Publication Pubmed ID :

2960578

Depositor Notes (Special Instructions) :

Immunoblotting: very good. Immunohistochemistry: good on cryosections and methanol and formaldehyde fixed tissues.

Collections :

Cardiac development,Enzymes,Human,Neurodevelopment,Skeletal muscle

Search Keywords :

Fambrough, D. M., ATPase, Ca(+2) fast twitch SR / SERCA1, Chicken, MIgG1, kappa light chain, Vertebrates/Rat/Mouse/Human, ATP2A1, SERCA1, AB_531812, monoclonal, Cardiac development/Enzymes/Human/Neurodevelopment/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA

Antigen Molecular Weight :

110 kDa

Gene :

ATP2A1

Alternate Gene Name(s) :

SERCA1

Uniprot ID :

P13585

Antibody Registry ID :

AB_531812

Synonyms (Alternate Clone Names) :

CAF2; 5D2

Immunogen :

Sarcoplasmic reticulum membrane vesicles preparation from adult chicken pectoralis major muscle.

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0 Ag8

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

0.5 ug/ml very good

Immunohistochemistry Pubmed IDs :

8040329

Immunofluorescence Pubmed IDs :

24177035 8415625 10213392

Western Blot Pubmed IDs :

27444300 21088205 19029185 19144634 17690892 19423840 21836041 11494272 21088205

Immunoprecipitation Pubmed IDs :

8415625

ELISA Pubmed IDs :

8040329

Pubmed IDs :

8219359 8219360 2526293 20188644 9003197

DSHB Growth Medium :

Iscove's

References (legacy) :

Dev. Dyn. 197, 169-188.; Dev. Dyn. 197, 189-202.; J. Physiol. 587.2, 443-460.; J. Biol. Chem. 284(12), 7811-7819.

more info or order :

Developmental Studies Hybridoma Bank product webpage