product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
ATPase, Ca(+2) fast twitch SR (avian) / SERCA1
catalog :
CaF1-5C3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CaF1-5C3
reactivity :
chicken
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
citations: 18
Reference |
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Inesi G, Ma H, Lewis D, Xu C. Ca2+ occlusion and gating function of Glu309 in the ADP-fluoroaluminate analog of the Ca2+-ATPase phosphoenzyme intermediate. J Biol Chem. 2004;279:31629-37 pubmed
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Biehn S, Czymmek K, Leavens K, Karin N. Expression of the sarco/endoplasmic Ca(2+)-ATPase, SERCA1a, in fibroblasts induces the formation of organelle membrane arrays. Exp Cell Res. 2004;292:78-88 pubmed
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Ma H, Sumbilla C, Farrance I, Klein M, Inesi G. Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes. Am J Physiol Cell Physiol. 2004;286:C556-64 pubmed
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Sumbilla C, Ma H, Seth M, Inesi G. Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes. Arch Biochem Biophys. 2003;415:178-83 pubmed
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Ma H, Inesi G, Toyoshima C. Substrate-induced conformational fit and headpiece closure in the Ca2+ATPase (SERCA). J Biol Chem. 2003;278:28938-43 pubmed
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Cavagna M, O Donnell J, Sumbilla C, Inesi G, Klein M. Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes. J Physiol. 2000;528 Pt 1:53-63 pubmed
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Zhang Z, Lewis D, Strock C, Inesi G, Nakasako M, Nomura H, et al. Detailed characterization of the cooperative mechanism of Ca(2+) binding and catalytic activation in the Ca(2+) transport (SERCA) ATPase. Biochemistry. 2000;39:8758-67 pubmed
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Sumbilla C, Lu L, Lewis D, Inesi G, Ishii T, Takeyasu K, et al. Ca(2+)-dependent and thapsigargin-inhibited phosphorylation of Na+,K(+)-ATPase catalytic domain following chimeric recombination with Ca(2+)-ATPase. J Biol Chem. 1993;268:21185-92 pubmed
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Airey J, Deerinck T, Ellisman M, Houenou L, Ivanenko A, Kenyon J, et al. Crooked neck dwarf (cn) mutant chicken skeletal muscle cells in low density primary cultures fail to express normal alpha ryanodine receptor and exhibit a partial mutant phenotype. Dev Dyn. 1993;197:189-202 pubmed
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Airey J, Baring M, Beck C, Chelliah Y, Deerinck T, Ellisman M, et al. Failure to make normal alpha ryanodine receptor is an early event associated with the crooked neck dwarf (cn) mutation in chicken. Dev Dyn. 1993;197:169-88 pubmed
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Lemas M, Hamrick M, Takeyasu K, Fambrough D. 26 amino acids of an extracellular domain of the Na,K-ATPase alpha-subunit are sufficient for assembly with the Na,K-ATPase beta-subunit. J Biol Chem. 1994;269:8255-9 pubmed
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Hussain A, Garnett C, Klein M, Tsai Wu J, Schneider M, Inesi G. Direct involvement of intracellular Ca2+ transport ATPase in the development of thapsigargin resistance by Chinese hamster lung fibroblasts. J Biol Chem. 1995;270:12140-6 pubmed
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Dumonteil E, Barre H, Meissner G. Expression of sarcoplasmic reticulum Ca2+ transport proteins in cold-acclimating ducklings. Am J Physiol. 1995;269:C955-60 pubmed
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Kaprielian Z, Fambrough D. Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle. Dev Biol. 1987;124:490-503 pubmed
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Karin N, Kaprielian Z, Fambrough D. Expression of avian Ca2+-ATPase in cultured mouse myogenic cells. Mol Cell Biol. 1989;9:1978-86 pubmed
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Campbell A, Kessler P, Sagara Y, Inesi G, Fambrough D. Nucleotide sequences of avian cardiac and brain SR/ER Ca(2+)-ATPases and functional comparisons with fast twitch Ca(2+)-ATPase. Calcium affinities and inhibitor effects. J Biol Chem. 1991;266:16050-5 pubmed
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product information
Internal ID :
2719
Name :
CaF1-5C3
Depositor Name :
Fambrough, D.M.
Depositor Institution :
Johns Hopkins School of Medicine
Depositor Website :
http://www.searlescholars.net/person/470
Date Deposited :
10/10/90
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
ATPase, Ca(+2) fast twitch SR (avian) / SERCA1
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Chicken,Duck
Species Tested (legacy) :
chicken specific
Initial Publication Pubmed ID :
2960578
Depositor Notes (Special Instructions) :
Immunoblotting: very good. Immunohistochemistry: good on cryosections and methanol and formaldehyde fixed tissues.
Collections :
Cardiac development,Enzymes,Neurodevelopment,Skeletal muscle
Search Keywords :
Fambrough, D. M., ATPase, Ca(+2) fast twitch SR (avian) / SERCA1, Chicken, MIgG1, kappa light chain, Chicken/Duck, ATP2A1, SERCA1, AB_531813, monoclonal, Cardiac development/Enzymes/Neurodevelopment/Skeletal muscle, Immunofluorescence/Western Blot/ Immunoprecipitation
Antigen Molecular Weight :
110 kDa
Gene :
ATP2A1
Alternate Gene Name(s) :
SERCA1
Uniprot ID :
P13585
Antibody Registry ID :
AB_531813
Synonyms (Alternate Clone Names) :
CAF3-5C3; 5C3; CAF3
Immunogen :
Sarcoplasmic reticulum membrane vesicles preparation from adult chicken pectoralis major muscle.
Immunogen Sequence :
Total protein
Clonality :
Monoclonal
Myeloma Strain :
SP2/0 Ag8
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
5 ug/ml very good
Immunofluorescence Pubmed IDs :
2960578 14720508 26314333 9530095 2526293 7744863 14592812 11018105 1831452
Western Blot Pubmed IDs :
2960578 14720508 9530095 2526293 10913287 12750373 8407954 7744863 14592812 11018105 12831840 7485465 1831452
Immunoprecipitation Pubmed IDs :
7907590
Pubmed IDs :
8219359 8219360 15150270
DSHB Growth Medium :
Iscove's
References (legacy) :
Dev. Biol. 124, 490-503.; Dev. Dyn. 197, 169-188.; Dev. Dyn. 197, 189-202.
more info or order :
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Developmental Studies Hybridoma Bank
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