product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Estrogen receptor beta (ER 2)
catalog :
CWK-F12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CWK-F12
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, western blot knockout validation
citations: 19
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; loading ...; fig 1c
  • immunoprecipitation; mouse; loading ...; fig 3b
Yuan B, Yang J, Dubeau L, Hu Y, Li R. A Phosphotyrosine Switch in Estrogen Receptor β Is Required for Mouse Ovarian Function. Front Cell Dev Biol. 2021;9:649087 pubmed publisher
Nelson A, Groen A, Miller J, Warren A, Holmes K, Tarulli G, et al. Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity. Mol Cell Endocrinol. 2017;440:138-150 pubmed publisher
Monsivais D, Dyson M, Yin P, Navarro A, Coon J, Pavone M, et al. Estrogen receptor β regulates endometriotic cell survival through serum and glucocorticoid-regulated kinase activation. Fertil Steril. 2016;105:1266-1273 pubmed publisher
Monsivais D, Dyson M, Yin P, Coon J, Navarro A, Feng G, et al. ER?- and prostaglandin E2-regulated pathways integrate cell proliferation via Ras-like and estrogen-regulated growth inhibitor in endometriosis. Mol Endocrinol. 2014;28:1304-15 pubmed publisher
Jiang Y, Gong P, Madak Erdogan Z, Martin T, Jeyakumar M, Carlson K, et al. Mechanisms enforcing the estrogen receptor ? selectivity of botanical estrogens. FASEB J. 2013;27:4406-18 pubmed publisher
Chang E, Frasor J, Komm B, Katzenellenbogen B. Impact of estrogen receptor beta on gene networks regulated by estrogen receptor alpha in breast cancer cells. Endocrinology. 2006;147:4831-42 pubmed
Duong V, Licznar A, Margueron R, Boulle N, Busson M, Lacroix M, et al. ERalpha and ERbeta expression and transcriptional activity are differentially regulated by HDAC inhibitors. Oncogene. 2006;25:1799-806 pubmed
Aghmesheh M, Edwards L, Clarke C, Byth K, Katzenellenbogen B, Russell P, et al. Expression of steroid hormone receptors in BRCA1-associated ovarian carcinomas. Gynecol Oncol. 2005;97:16-25 pubmed
Chen J, Delannoy M, Cooke C, Yager J. Mitochondrial localization of ERalpha and ERbeta in human MCF7 cells. Am J Physiol Endocrinol Metab. 2004;286:E1011-22 pubmed
Tonetti D, Rubenstein R, DeLeon M, Zhao H, Pappas S, Bentrem D, et al. Stable transfection of an estrogen receptor beta cDNA isoform into MDA-MB-231 breast cancer cells. J Steroid Biochem Mol Biol. 2003;87:47-55 pubmed
Watanabe T, Akishita M, He H, Miyahara Y, Nagano K, Nakaoka T, et al. 17 beta-estradiol inhibits cardiac fibroblast growth through both subtypes of estrogen receptor. Biochem Biophys Res Commun. 2003;311:454-9 pubmed
Watanabe T, Akishita M, Nakaoka T, Kozaki K, Miyahara Y, He H, et al. Estrogen receptor beta mediates the inhibitory effect of estradiol on vascular smooth muscle cell proliferation. Cardiovasc Res. 2003;59:734-44 pubmed
Bukovsky A, Caudle M, Cekanova M, Fernando R, Wimalasena J, Foster J, et al. Placental expression of estrogen receptor beta and its hormone binding variant--comparison with estrogen receptor alpha and a role for estrogen receptors in asymmetric division and differentiation of estrogen-dependent cells. Reprod Biol Endocrinol. 2003;1:36 pubmed
Kulakosky P, Klinge C. Maximizing production of estrogen receptor beta with the baculovirus expression system. Biotechniques. 2003;34:334-8, 340-3 pubmed
Kowalski A, Graddy L, Vale Cruz D, Choi I, Katzenellenbogen B, Simmen F, et al. Molecular cloning of porcine estrogen receptor-beta complementary DNAs and developmental expression in periimplantation embryos. Biol Reprod. 2002;66:760-9 pubmed
Loven M, Likhite V, Choi I, Nardulli A. Estrogen response elements alter coactivator recruitment through allosteric modulation of estrogen receptor beta conformation. J Biol Chem. 2001;276:45282-8 pubmed
Lazennec G, Bresson D, Lucas A, Chauveau C, Vignon F. ER beta inhibits proliferation and invasion of breast cancer cells. Endocrinology. 2001;142:4120-30 pubmed
Loven M, Wood J, Nardulli A. Interaction of estrogen receptors alpha and beta with estrogen response elements. Mol Cell Endocrinol. 2001;181:151-63 pubmed
Choi I, Ko C, Park Sarge O, Nie R, Hess R, Graves C, et al. Human estrogen receptor beta-specific monoclonal antibodies: characterization and use in studies of estrogen receptor beta protein expression in reproductive tissues. Mol Cell Endocrinol. 2001;181:139-50 pubmed
product information
Internal ID :
27204
Name :
CWK-F12
Depositor Name :
Katzenellenbogen, B.S.
Depositor Institution :
University of Illinois
Date Deposited :
3/30/17
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
Antigen :
Estrogen receptor beta (ER 2)
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG2b
Positive Tested Species Reactivity :
Human,Mouse,Porcine,Rat
Initial Publication Pubmed ID :
11476948 (Choi et al 2001)
Depositor Notes (Special Instructions) :
This monoclonal antibody has recently be shown to be specific for ER beta unlike other commonly used anti-ER beta antibodies (see Nelson et al 2017 PMID 27889472).
Collections :
Human,Nucleus,Transcription factors
Search Keywords :
Katzenellenbogen, B.S., Estrogen receptor beta (ER 2), Human, MIgG2b, Human/Rat/Mouse/Porcine, ESR2, Erb, NR3A2, , Monoclonal, Human/Nucleus/Transcription factors, Western blot/ELISA/Immunoprecipitation/Immunohistochemistry/FFPE/Function blocking/Chromatin immunoprecipitation/Immunofluorescence, AB_2722105
Antigen Molecular Weight :
59 kDa
Gene :
ESR2
Alternate Gene Name(s) :
Erb, NR3A2
Uniprot ID :
Q92731
Antibody Registry ID :
AB_2722105
Immunogen :
E2 ligand-binding domain
Immunogen Sequence :
a.a. 256-505
Clonality :
Monoclonal
Myeloma Strain :
SP 2/0
Epitope Mapped :
No
Epitope Map PubMed ID :
11476948
Recommended Applications :
Chromatin Immunoprecipitation,ELISA,FFPE,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunohistochemistry Pubmed IDs :
11476948 27889472 11517191 11870084 12740031 15790432
Immunofluorescence Pubmed IDs :
14736707
Western Blot Pubmed IDs :
11476948 27889472 11517191 11476949 11870084 14736707 23882126 14630090 16158045 12613256 16809442 12740031 14592435 14499875 24992181
Immunoprecipitation Pubmed IDs :
11476948
Chromatin Immunoprecipitation Pubmed IDs :
23882126 26827666 24992181
ELISA Pubmed IDs :
11476948
FFPE Pubmed IDs :
11476948 27889472 11870084 15790432
Function Blocking Pubmed IDs :
11517191 11574541
Additional Information :
RRID: AB_2722105
DSHB Growth Medium :
Iscove's
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US