troponin T (cardiac/slow) | Developmental Studies Hybridoma Bank CT3 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

troponin T (cardiac/slow)

catalog :

CT3

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CT3

reactivity :

human, mouse, brown rat, bantam, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:10; loading ...; fig 3b immunohistochemistry - frozen section; bantam; 1:10; loading ...; fig 1h	Tang W, Martik M, Li Y, Bronner M. Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish. elife. 2019;8: pubmed publisher
immunocytochemistry; mouse; fig 5b immunohistochemistry; brown rat; loading ...; fig 7c	Maltabe V, Barka E, Kontonika M, Florou D, Kouvara Pritsouli M, Roumpi M, et al. Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem Cells Int. 2016;2016:8305624 pubmed publisher
western blot; bantam; 1:100; fig 2d	Blech Hermoni Y, Sullivan C, Jenkins M, Wessely O, Ladd A. CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart. Dev Dyn. 2016;245:854-73 pubmed publisher
immunocytochemistry; human; 4 ug/ml; fig 1	Eng G, Lee B, Protas L, Gagliardi M, Brown K, Kass R, et al. Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes. Nat Commun. 2016;7:10312 pubmed publisher
immunohistochemistry - paraffin section; mouse	Geula S, Moshitch Moshkovitz S, Dominissini D, Mansour A, Kol N, Salmon Divon M, et al. Stem cells. m6A mRNA methylation facilitates resolution of naÃ¯ve pluripotency toward differentiation. Science. 2015;347:1002-6 pubmed publisher
western blot; mouse; 1:500	Yuan B, Wan P, Chu D, Nie J, Cao Y, Luo W, et al. A cardiomyocyte-specific Wdr1 knockout demonstrates essential functional roles for actin disassembly during myocardial growth and maintenance in mice. Am J Pathol. 2014;184:1967-80 pubmed publisher
	Liao S, Dong W, Lv L, Guo H, Yang J, Zhao H, et al. Heart regeneration in adult Xenopus tropicalis after apical resection. Cell Biosci. 2017;7:70 pubmed publisher
	Chan S, Chan H, Kyba M. Heterogeneity of Mesp1+ mesoderm revealed by single-cell RNA-seq. Biochem Biophys Res Commun. 2016;474:469-475 pubmed publisher
	Haremaki T, Sridharan J, Dvora S, Weinstein D. Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3. Dev Dyn. 2010;239:1977-87 pubmed publisher
	Jin J, Chong S. Localization of the two tropomyosin-binding sites of troponin T. Arch Biochem Biophys. 2010;500:144-50 pubmed publisher
	Drenckhahn J, Schwarz Q, Gray S, Laskowski A, Kiriazis H, Ming Z, et al. Compensatory growth of healthy cardiac cells in the presence of diseased cells restores tissue homeostasis during heart development. Dev Cell. 2008;15:521-33 pubmed publisher
	Mukherjee A, Wilson E, Rotwein P. Insulin-like growth factor (IGF) binding protein-5 blocks skeletal muscle differentiation by inhibiting IGF actions. Mol Endocrinol. 2008;22:206-15 pubmed
	Dagle J, Sabel J, Littig J, Sutherland L, Kolker S, Weeks D. Pitx2c attenuation results in cardiac defects and abnormalities of intestinal orientation in developing Xenopus laevis. Dev Biol. 2003;262:268-81 pubmed
	Jin J, Brotto M, Hossain M, Huang Q, Brotto L, Nosek T, et al. Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy. J Biol Chem. 2003;278:26159-65 pubmed
	Dube M, Huot M, Khandjian E. Muscle specific fragile X related protein 1 isoforms are sequestered in the nucleus of undifferentiated myoblast. BMC Genet. 2000;1:4 pubmed
	Jin J, Chen A, Ogut O, Huang Q. Conformational modulation of slow skeletal muscle troponin T by an NH(2)-terminal metal-binding extension. Am J Physiol Cell Physiol. 2000;279:C1067-77 pubmed
	Kolker S, Tajchman U, Weeks D. Confocal imaging of early heart development in Xenopus laevis. Dev Biol. 2000;218:64-73 pubmed
	Wang D, Reiter R, Lin J, Wang Q, Williams H, Krob S, et al. Requirement of a novel gene, Xin, in cardiac morphogenesis. Development. 1999;126:1281-94 pubmed
	Jin J. Alternative RNA splicing-generated cardiac troponin T isoform switching: a non-heart-restricted genetic programming synchronized in developing cardiac and skeletal muscles. Biochem Biophys Res Commun. 1996;225:883-9 pubmed
	Warren K, Lin J. Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells. J Muscle Res Cell Motil. 1993;14:619-32 pubmed
	Swiderski R, Solursh M. Precocious appearance of cardiac troponin T pre-mRNAs during early avian embryonic skeletal muscle development in ovo. Dev Biol. 1990;140:73-82 pubmed

product information

Internal ID :

2993

Name :

CT3

Depositor Name :

Lin, J.J.-C.

Depositor Institution :

University of Iowa

Date Deposited :

1/2/96

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

troponin T (cardiac/slow)

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Broad species,Chicken,Fish,Human,Mouse,Rabbit,Rat,Xenopus

Species Tested (legacy) :

broad-species specificity, Xenopus, rat, mouse, human

Initial Publication Pubmed ID :

2358124

Depositor Notes (Special Instructions) :

CT3 was initially published in: Jin, J., Lin, J.-C., and Lin, J.J.-C. (1990). Troponin T isoform switching during heart development. Ann. NY Acad. Sci. 588, 393-396.

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Muscular dystrophy,Skeletal muscle,Xenopus

Search Keywords :

Lin J.J.-C., troponin T (cardiac/slow), Bovine, MIgG2a, broad species/Xenopus/rat/mouse/human/chicken/rabbit/fish, TNNT2, , AB_528495, monoclonal, Cardiac development/Cytoskeletal elements/Muscular dystrophy/Xenopus/Cell markers/Skeletal muscle/Human, Immunohistochemistry/Immunofluorescence/Western Blot/FFPE

Antigen Molecular Weight :

Predicted (cTnT): 34 kDa; Apparent (cTnT): 43kDa

Gene :

TNNT2

Uniprot ID :

P13789

Antibody Registry ID :

AB_528495

Immunogen :

Purified cardiac troponin T

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 94-178

Epitope Map PubMed ID :

20529660

Recommended Applications :

FFPE,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Single band from cardiac muscle

Immunohistochemistry Pubmed IDs :

20549732 12732643 27144987 27144987

Immunofluorescence Pubmed IDs :

2358124 8126222 10644411 14550790 18854137 10021346 17885206 29255592

Western Blot Pubmed IDs :

8126222 11178106 8780706 12732643 11003587 17885206 20529660

FACS Pubmed IDs :

27131741

Additional Information :

CT3 recognizes the embryonic and adult cardiac isforms [PMID: 2358124]. CT3 cross-react with slow skeletal muscle TnT but doesn't recognize fast skeletal muscle TnT [PMID: 12732643].

DSHB Growth Medium :

RPMI

more info or order :

Developmental Studies Hybridoma Bank product webpage